Harnessing the power of an X-ray laser for serial crystallography of membrane proteins crystallized in lipidic cubic phase

Autor: Ming-Yue Lee, James Geiger, Andrii Ishchenko, Gye Won Han, Anton Barty, Thomas A. White, Cornelius Gati, Alexander Batyuk, Mark S. Hunter, Andrew Aquila, Sébastien Boutet, Uwe Weierstall, Vadim Cherezov, Wei Liu

Publikováno v: IUCrJ, Vol 7, Iss 6, Pp 976-984 (2020)

Serial femtosecond crystallography (SFX) with X-ray free-electron lasers (XFELs) has proven highly successful for structure determination of challenging membrane proteins crystallized in lipidic cubic phase; however, like most techniques, it has limi

Externí odkaz: https://doaj.org/article/0f99c930cfd741b495646d084e600fcc

A solution NMR view of lipidic cubic phases: Structure, dynamics, and beyond

Autor: Thomas G Meikle, David W Keizer, Frances Separovic, Shenggen Yao

Publikováno v: BBA Advances, Vol 2, Iss , Pp 100062- (2022)

Nuclear magnetic resonance (NMR) spectroscopy is well-established nowadays for the elucidation of the 3D structures of proteins and protein complexes, the evaluation of biomolecular dynamics with atomistic resolution across a range of time scales, th

Externí odkaz: https://doaj.org/article/ad2283cb7a8b4c88af5e59aa83b5c5d1

Flavin‐Helicene Amphiphilic Hybrids: Synthesis, Characterization, and Preparation of Surface‐Supported Films

Autor: Adrianna Cytryniak, Renata Bilewicz, Jan Storch, Jeanne Crassous, Radek Cibulka, Ivana Císařová, Martin Jakubec, Jan Vacek, Jan Hrbac, David Novak, Ludovic Favereau, Martina Zatloukalová, Jaroslav Žádný

Publikováno v: ChemPlusChem
ChemPlusChem, 2021, 86 (7), pp.982-990. ⟨10.1002/cplu.202100092⟩
ChemPlusChem, Wiley, 2021, ⟨10.1002/cplu.202100092⟩

International audience; This work reports on the preparation and structural characterization of flavo[7]helicene 1 (flavin-[7]helicene conjugate), which was subsequently characterized at the molecular level in either an aqueous environment or an orga

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4401114fec388229b677795a44cd7448
https://doi.org/10.1002/cplu.202100092

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Lipidic cubic phase serial millisecond crystallography using synchrotron radiation

Autor: Markus Metz, Vadim Cherezov, Peter Berntsen, Dingjie Wang, Richard Neutze, Wenting Wu, Gebhard F. X. Schertler, Linda C. Johansson, Isabel Moraes, Dominik Oberthuer, Thomas A. White, Henry N. Chapman, Valerie Panneels, Kathrin Jaeger, Petra Båth, Daniel James, Anastasya Shilova, Michael Heymann, Manfred Burghammer, Cecilia Wickstrand, Garrett Nelson, Uwe Weierstall, Joerg Standfuss, Nadia A. Zatsepin, Przemyslaw Nogly, Haiguang Liu, John David Spence

Publikováno v: International Union of Crystallography journal
International Union of Crystallography journal, International Union of Crystallography 2015, 2, pp.168-176. ⟨10.1107/S2052252514026487⟩
IUCrJ
IUCrJ, Vol 2, Iss 2, Pp 168-176 (2015)
IUCrJ, 2 (2)
IUCrJ 2(2), 1-9 (2015). doi:10.1107/S2052252514026487
'IUCrJ ', vol: 2, pages: 168-176 (2015)

Lipidic cubic phases (LCPs) have emerged as successful matrixes for the crystallization of membrane proteins. Moreover, the viscous LCP also provides a highly effective delivery medium for serial femtosecond crystallography (SFX) at X-ray free-electr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::743e33033daab3499b01edff84f669c3
https://doi.org/10.1107/s2052252514026487