Unraveling productivity-enhancing genes in Chinese hamster ovary cells via CRISPR activation screening using recombinase-mediated cassette exchange system

Autor: Baek, Minhye, Kim, Che Lin, Kim, Su Hyun, la Cour Karottki, Karen Julie, Hefzi, Hooman, Grav, Lise Marie, Pedersen, Lasse Ebdrup, Lewis, Nathan E., Lee, Jae Seong, Lee, Gyun Min

Publikováno v: In Metabolic Engineering January 2025 87:11-20

Unraveling productivity-enhancing genes in Chinese hamster ovary cells via CRISPR activation screening using recombinase-mediated cassette exchange system.

Autor: Baek M; Department of Biological Sciences, KAIST, Daejeon, 34141, Republic of Korea., Kim CL; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Lyngby, Denmark., Kim SH; Department of Biological Sciences, KAIST, Daejeon, 34141, Republic of Korea., la Cour Karottki KJ; Departments of Pediatrics and Bioengineering, University of California, San Diego, USA., Hefzi H; Departments of Pediatrics and Bioengineering, University of California, San Diego, USA., Grav LM; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Lyngby, Denmark., Pedersen LE; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Lyngby, Denmark., Lewis NE; Departments of Pediatrics and Bioengineering, University of California, San Diego, USA., Lee JS; Department of Molecular Science and Technology, Ajou University, Suwon, 16499, Republic of Korea. Electronic address: jaeseonglee@ajou.ac.kr., Lee GM; Department of Biological Sciences, KAIST, Daejeon, 34141, Republic of Korea. Electronic address: gyunminlee@kaist.ac.kr.

Publikováno v: Metabolic engineering [Metab Eng] 2024 Nov 19; Vol. 87, pp. 11-20. Date of Electronic Publication: 2024 Nov 19.

Reduced apoptosis in Chinese hamster ovary cells via optimized CRISPR interference.

Autor: Xiong K; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Denmark., Marquart KF; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Denmark., la Cour Karottki KJ; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Denmark., Li S; Department of Pediatrics, University of California, San Diego, California.; Department of Bioengineering, University of California, San Diego, California.; The Novo Nordisk Foundation Center for Biosustainability, University of California, San Diego, California., Shamie I; Department of Pediatrics, University of California, San Diego, California.; The Novo Nordisk Foundation Center for Biosustainability, University of California, San Diego, California., Lee JS; Department of Molecular Science and Technology, Ajou University, Suwon, Republic of Korea., Gerling S; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Denmark., Yeo NC; Department of Pharmacology and Toxicology, Precision Medicine Institute, University of Alabama, Birmingham, Alabama., Chavez A; Department of Pathology and Cell Biology, Columbia University College of Physicians and Surgeons, New York, New York., Lee GM; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Denmark.; Department of Biological Sciences, KAIST, Daejeon, Republic of Korea., Lewis NE; Department of Pediatrics, University of California, San Diego, California.; Department of Bioengineering, University of California, San Diego, California.; The Novo Nordisk Foundation Center for Biosustainability, University of California, San Diego, California., Kildegaard HF; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Denmark.

Publikováno v: Biotechnology and bioengineering [Biotechnol Bioeng] 2019 Jul; Vol. 116 (7), pp. 1813-1819. Date of Electronic Publication: 2019 Apr 02.