Výsledky vyhledávání - "integrative vector"

Akademický článek

Development of Integrated Vectors with Strong Constitutive Promoters for High-Yield Antibiotic Production in Mangrove-Derived Streptomyces

Autor: Mingxia Zhao, Zhiqiang Yang, Xinyue Li, Yaqi Liu, Yingying Zhang, Mengqian Zhang, Yangli Li, Xincheng Wang, Zixin Deng, Kui Hong, Dongqing Zhu

Publikováno v: Marine Drugs, Vol 22, Iss 2, p 94 (2024)

It is important to improve the production of bioactive secondary products for drug development. The Escherichia coli—Streptomyces shuttle vector pSET152 and its derived vector pIB139 containing a strong constitutive promoter ermEp* are commonly use

Externí odkaz: https://doaj.org/article/f95eb8a1857b4457ab643026a1186346

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Akademický článek

Engineering integrative vectors based on phage site-specific recombination mechanism for Lactococcus lactis

Autor: Innanurdiani Koko, Adelene Ai-Lian Song, Mas Jaffri Masarudin, Raha Abdul Rahim

Publikováno v: BMC Biotechnology, Vol 19, Iss 1, Pp 1-12 (2019)

Abstract Background Site-specific integration system allows foreign DNA to be integrated into the specific site of the host genome, enabling stable expression of heterologous protein. In this study, integrative vectors for secretion and surface displ

Externí odkaz: https://doaj.org/article/ac19a90c5b9a455184e2aecad87e8675

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Akademický článek

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Akademický článek

Construction of a New Phage Integration Vector pFIV-Val for Use in Different Francisella Species

Autor: Hana Tlapák, Kristin Köppen, Kerstin Rydzewski, Roland Grunow, Klaus Heuner

Publikováno v: Frontiers in Cellular and Infection Microbiology, Vol 8 (2018)

We recently identified and described a putative prophage on the genomic island FhaGI-1 located within the genome of Francisella hispaniensis AS02-814 (F. tularensis subsp. novicida-like 3523). In this study, we constructed two variants of a Francisel

Externí odkaz: https://doaj.org/article/3c1117ae7b334dd4b41693cd8a3a0aba

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Akademický článek

A set of novel CRISPR-based integrative vectors for Saccharomyces cerevisiae.

Autor: Daniels, P.W., Mukherjee, A., Goldman, Alastair S.H., Hu, B.

Yes
Integrating a desired DNA sequence into the yeast genomes is a widely-used genetic manipulation in the budding yeast Saccharomyces cerevisiae. The conventional integration method is to use an integrative plasmid such as pRS or YIplac series

Externí odkaz: http://hdl.handle.net/10454/17333

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Engineering integrative vectors based on phage site-specific recombination mechanism for Lactococcus lactis

Autor: Adelene Ai-Lian Song, Mas Jaffri Masarudin, Raha Abdul Rahim, Innanurdiani Koko

Publikováno v: BMC Biotechnology, Vol 19, Iss 1, Pp 1-12 (2019)
BMC Biotechnology

Background Site-specific integration system allows foreign DNA to be integrated into the specific site of the host genome, enabling stable expression of heterologous protein. In this study, integrative vectors for secretion and surface display of pro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::da4de7783cbf72736a628baca08b1c66
http://link.springer.com/article/10.1186/s12896-019-0575-x

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Induced pluripotent stem cell line (INSAi001-A) from a Gaucher disease type 3 patient compound heterozygote for mutations in the GBA1 gene

Autor: Ana Joana Duarte, Olga Amaral, Diogo Ribeiro, José Bragança, Luciana Moreira, Renato Santos

Publikováno v: Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP
Stem Cell Research, Vol 41, Iss, Pp-(2019)

The authors thank Hildeberto Correia (Department of Human Genetics in Lisbon), for the karyotype analysis; José Ferrão (Department of Human Genetics in Lisbon), for the STR analysis; the collaboration of the central core services of Department of H

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::416335e9fae9dd34ba8491464ece58a0
https://hdl.handle.net/10400.18/6527

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Construction of a New Phage Integration Vector pFIV-Val for Use in Different Francisella Species

Autor: Kristin Köppen, Kerstin Rydzewski, Klaus Heuner, Hana Tlapák, Roland Grunow

Publikováno v: Frontiers in Cellular and Infection Microbiology, Vol 8 (2018)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0add112379e753ba5bec016ce292387b
http://journal.frontiersin.org/article/10.3389/fcimb.2018.00075/full

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Construction of a New Phage Integration Vector pFIV-Val for Use in Different

Autor: Hana, Tlapák, Kristin, Köppen, Kerstin, Rydzewski, Roland, Grunow, Klaus, Heuner

Publikováno v: Frontiers in Cellular and Infection Microbiology

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::7e76d9a7e251a8f6ece5ddb940ee1287
https://pubmed.ncbi.nlm.nih.gov/29594068

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A new tool for cloning and gene expression in Streptococcus pneumoniae

Autor: Andreia Aires, Susana Domingues, Paloma López, Mari Luz Mohedano, Cecília M. Arraiano

Publikováno v: Digital.CSIC. Repositorio Institucional del CSIC
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18 p.-4 fig.-1 tab.
A new replicon suitable for cloning and gene expression was successfully introduced into Streptococcus pneumoniae. The non-integrative lactococcal vectors pIL253 (higher-copy) and pIL252 (lower-copy), which are based on the p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::223ac679d241306f564eae52b7729eaf
https://doi.org/10.1016/j.plasmid.2013.05.005

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