Thermal tuning of protein hydration in a hyperthermophilic enzyme

Autor: Giuliana Fusco, Carmen Biancaniello, Michail D. Vrettas, Alfonso De Simone

Publikováno v: Frontiers in Molecular Biosciences, Vol 9 (2022)

Water at the protein surface is an active biological molecule that plays a critical role in many functional processes. Using NMR-restrained MD simulations, we here addressed how protein hydration is tuned at high biological temperatures by analysing

Externí odkaz: https://doaj.org/article/bfb9d980bcfb438eb3f1c1b9107ab2b9

Differential Effects of Hydrophobic Core Packing Residues for Thermodynamic and Mechanical Stability of a Hyperthermophilic Protein

Autor: Michael C. Wilson, Emanuele Paci, Toni Hoffmann, Lorna Dougan, Katarzyna M. Tych, Megan L. Hughes, Matthew Batchelor, David J. Brockwell

Proteins from organisms that have adapted to environmental extremes provide attractive systems to explore and determine the origins of protein stability. Improved hydrophobic core packing and decreased loop-length flexibility can increase the thermod

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9024b30f76f2a29575fc148175736109
http://hdl.handle.net/11585/885043

Comprehensive analysis of surface charged residues involved in thermal stability in Alicyclobacillus acidocaldarius esterase 2

Autor: Giuseppe Manco, Luigi Mandrich, Margherita Pezzullo, Mosè Rossi, Roberto Nucci, Pompea Del Vecchio

Publikováno v: 11th International Meeting on Cholinesterases, pp. 47–58, Kazan, Russia, 4-9 Giugno 2012
info:cnr-pdr/source/autori:PEZZULLO M., DEL VECCHIO P., MANDRICH L., NCCI R., ROSSI M., MANCO G.,/congresso_nome:11th International Meeting on Cholinesterases/congresso_luogo:Kazan, Russia/congresso_data:4-9 Giugno 2012/anno:2012/pagina_da:47/pagina_a:58/intervallo_pagine:47–58

Here we report a comprehensive analysis through alanine-scanning mutagenesis of the contribution of surface ion pairs to the thermal stability of Alicyclobacillus acidocaldarius esterase 2 (EST2). We produced 16 single mutants, 4 double mutants corre

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::da8006629b6c291eed45b8d16c032ab2
https://publications.cnr.it/doc/209567

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