DNA nuclear targeting sequences for enhanced non-viral gene transfer: An in vitro and in vivo study

Autor: Yann T. Le Guen, Chantal Pichon, Philippe Guégan, Kévin Pluchon, Tanguy Haute, Sandrine Quemener, Juliette Ropars, Patrick Midoux, Tony Le Gall, Tristan Montier

Publikováno v: Molecular Therapy: Nucleic Acids, Vol 24, Iss , Pp 477-486 (2021)

An important bottleneck for non-viral gene transfer commonly relates to translocation of nucleic acids into the nuclear compartment of target cells. So-called 3NFs are optimized short nucleotide sequences able to interact with the transcription facto

Externí odkaz: https://doaj.org/article/cbbf416e03b24cf5ad0af59a2047b63f

DNA nuclear targeting sequences for enhanced non-viral gene transfer: An in vitro and in vivo study

Autor: Tristan Montier, Philippe Guégan, Sandrine Quemener, Kévin Pluchon, Juliette Ropars, Chantal Pichon, Tanguy Haute, Yann Thierry Le Guen, Tony Le Gall, Patrick Midoux

Publikováno v: Molecular Therapy: Nucleic Acids, Vol 24, Iss, Pp 477-486 (2021)
Molecular Therapy-Nucleic Acids
Molecular Therapy-Nucleic Acids, 2021, 24, pp.477-486. ⟨10.1016/j.omtn.2021.03.012⟩
Molecular Therapy-Nucleic Acids, Elsevier, 2021, 24, pp.477-486. ⟨10.1016/j.omtn.2021.03.012⟩

International audience; An important bottleneck for non-viral gene transfer commonly relates to translocation of nucleic acids into the nuclear compartment of target cells. So-called 3NFs are optimized short nucleotide sequences able to interact with

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9c4a5b1f27897bb492e888401d28fe8f
http://www.sciencedirect.com/science/article/pii/S2162253121000834

Non-viral gene transfer to skeletal muscles using hydrodynamic limb vein injection : optimization strategies and application in the mouse

Autor: Le Guen, Yann

Publikováno v: Médecine humaine et pathologie. Université de Bretagne occidentale-Brest, 2019. Français. ⟨NNT : 2019BRES0108⟩

Hydrodynamic Limb Vein injection (HLV) is a physical loco-regional gene delivery method. In fact, it allows to treat the whole musculature of a given limb isolated by a tourniquet thanks to the delivery of a large volume containing nucleic acid const

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od______1398::7f0ee12feb7d34c499bee2dc31a6fb80
https://tel.archives-ouvertes.fr/tel-03621300/file/These-2019-BS-Technologies_biomedicales-LE_GUEN_Yann.pdf

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Localization of exogenous DNA to mitochondria in skeletal muscle following hydrodynamic limb vein injection

Autor: Tsutomu Kanefuji, Yuma Yamada, Yukari Yasuzaki, Hideyoshi Harashima

Publikováno v: Journal of controlled release : official journal of the Controlled Release Society. 172(3)

Mitochondrial genetic disorders are a major cause of mitochondrial diseases. It is therefore likely that mitochondrial gene therapy will be useful for the treatment of such diseases. Here, we report on the possibility of mitochondrial gene delivery i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::efd13c8e9f733e21d0683944aeee3363
https://pubmed.ncbi.nlm.nih.gov/24100263