Ovine placental lactogen-induced heterodimerization of ovine growth hormone and prolactin receptors in living cells is demonstrated by fluorescence resonance energy transfer microscopy and leads to prolonged phosphorylation of signal transducer and activator of transcription (STAT)1 and STAT3

Autor: Brian Herman, Stuart J. Frank, Jean Djiane, Arieh Gertler, Eva Biener, Victoria E. Centonze, Cyril Martin, Nathalie Daniel

Publikováno v: Endocrinology
Endocrinology, Endocrine Society, 2003, 144 (8), pp.3532-3540. ⟨10.1210/en.2003-0096#sthash.x2nvVx34.dpuf⟩
Endocrinology 8 (144), 3532-3540. (2003)

HEK-293T cells transiently transfected with ovine (o) GH receptor (GHR) and prolactin receptor (PRLR) constructs respectively tagged downstream with cyan or yellow fluorescent proteins were used to study ovine placental lactogen (oPL)-stimulated hete

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f3fe077af19ddb8f6ed1a4aee248619f
https://pubmed.ncbi.nlm.nih.gov/12865335

Selective modification of recombinant bovine placental lactogen by site-directed mutagenesis at its C terminus

Autor: Gwen G. Krivi, Edna Sakal, N.R. Staten, Arieh Gertler, Jean Djiane, Russell E. McKinnie, Dorit Vashdi-Elberg

Publikováno v: Journal of Biological Chemistry
Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 1996, 271 (10), pp.5558-5564. ⟨10.1074/jbc.271.10.5558⟩
Journal of Biological Chemistry 10 (271), 5558-5564. (1996)

Five recombinant analogues of bovine placental lactogen (bPL) ((bPL(S184H), bPL(S187A), bPL(S187F), bPL(T188F), bPL(T188F,I190F)) were prepared, expressed in Escherichia coli, and purified to homogeneity. Circular dichroism analysis revealed no or mi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::be53e930dae3b51d98a9411dd5133b78
https://hal.inrae.fr/hal-02687643