Zobrazeno 1 - 10
of 2 739
pro vyhledávání: '"homology directed repair"'
Publikováno v:
Fungal Biology and Biotechnology, Vol 11, Iss 1, Pp 1-9 (2024)
Abstract Non-homologous end joining (NHEJ) and homology-directed repair (HDR) are two mechanisms in filamentous fungi to repair DNA damages. NHEJ is the dominant response pathway to rapidly join DNA double-strand breaks, but often leads to insertions
Externí odkaz:
https://doaj.org/article/52347a8ee045481ba65569f2ffe7bf83
Autor:
Hui Shi, Lei Li, Shuangshuang Mu, Shixue Gou, Xiaoyi Liu, Fangbing Chen, Menglong Chen, Qin Jin, Liangxue Lai, Kepin Wang
Publikováno v:
BMC Biology, Vol 22, Iss 1, Pp 1-16 (2024)
Abstract Background Many efforts have been made to improve the precision of Cas9-mediated gene editing through increasing knock-in efficiency and decreasing byproducts, which proved to be challenging. Results Here, we have developed a human exonuclea
Externí odkaz:
https://doaj.org/article/63f8d4db211341e188077f960aa406ff
Autor:
Baolei Yuan, Chongwei Bi, Yeteng Tian, Jincheng Wang, Yiqing Jin, Khaled Alsayegh, Muhammad Tehseen, Gang Yi, Xuan Zhou, Yanjiao Shao, Fernanda Vargas Romero, Wolfgang Fischle, Juan Carlos Izpisua Belmonte, Samir Hamdan, Yanyi Huang, Mo Li
Publikováno v:
BMC Biology, Vol 22, Iss 1, Pp 1-15 (2024)
Abstract Background CRISPR-Cas9 genome editing often induces unintended, large genomic rearrangements, posing potential safety risks. However, there are no methods for mitigating these risks. Results Using long-read individual-molecule sequencing (ID
Externí odkaz:
https://doaj.org/article/d1681b87d8e041b8a7a543e11f0f1746
Autor:
Shiqian Li, Yafei Wang, Miesje van der Stoel, Xin Zhou, Shrinidhi Madhusudan, Kristiina Kanerva, Van Dien Nguyen, Nazli Eskici, Vesa M. Olkkonen, You Zhou, Taneli Raivio, Elina Ikonen
Publikováno v:
Genome Biology, Vol 25, Iss 1, Pp 1-26 (2024)
Abstract Recent developments in auxin-inducible degron (AID) technology have increased its popularity for chemogenetic control of proteolysis. However, generation of human AID cell lines is challenging, especially in human embryonic stem cells (hESCs
Externí odkaz:
https://doaj.org/article/b386292dc07c45e88b124344bcaa5ff8
Autor:
Yao Yuan, Lingqi Yu, Xudong Zhuang, Dongjing Wen, Jin He, Jingmei Hong, Jiayu Xie, Shengan Ling, Xiaoyue Du, Wenfeng Chen, Xinrui Wang
Publikováno v:
Neural Regeneration Research, Vol 20, Iss 1, Pp 265-276 (2023)
Certain amino acids changes in the human Na+/K+-ATPase pump, ATPase Na+/K+ transporting subunit alpha 1 (ATP1A1), cause Charcot-Marie-Tooth disease type 2 (CMT2) disease and refractory seizures. To develop in vivo models to study the role of Na+/K+-A
Externí odkaz:
https://doaj.org/article/686df9f3d48f4a749e2a26378cf2680c
Publikováno v:
Cell & Bioscience, Vol 13, Iss 1, Pp 1-13 (2023)
Abstract Background Gene knock‐in (KI) in animal cells via homology‐directed repair (HDR) is an inefficient process, requiring a laborious work for screening from few modified cells. HDR tends to occur in the S and G2/M phases of cell cycle; ther
Externí odkaz:
https://doaj.org/article/7f6c95039fc244f7889e6d7d1689790e
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 35, Iss 1, Pp 102124- (2024)
In genome editing, it is important to avoid off-target mutations so as to reduce unexpected side effects, especially for therapeutic applications. Recently, several high-fidelity versions of SpCas9 have been developed to reduce off-target mutations.
Externí odkaz:
https://doaj.org/article/51d5c0e3ef8a44fca6e7053d0086f249
Autor:
Sathya Neelature Sriramareddy, Majeed Jamakhani, Léa Vilanova, Hélène Brossel, Bernard Staumont, Malik Hamaidia
Publikováno v:
Frontiers in Oncology, Vol 14 (2024)
BackgroundAlthough the incidence of anaplastic thyroid carcinoma (ATC) is low (2.5% of thyroid cancer cases), this cancer has a very poor prognosis (survival rates < 5 months) and accounts for 14–39% of deaths. Conventional therapies based on surge
Externí odkaz:
https://doaj.org/article/14576f84cc1a4985b4ffe48956317892
Autor:
Kathryn O. Farley, Catherine A. Forbes, Nicole C. Shaw, Emma Kuzminski, Michelle Ward, Gareth Baynam, Timo Lassmann, Vanessa S. Fear
Publikováno v:
HGG Advances, Vol 5, Iss 1, Pp 100257- (2024)
Summary: An estimated 3.5%–5.9% of the global population live with rare diseases, and approximately 80% of these diseases have a genetic cause. Rare genetic diseases are difficult to diagnose, with some affected individuals experiencing diagnostic
Externí odkaz:
https://doaj.org/article/05ebced0f70c428089b7189aa4addd42
Publikováno v:
Current Issues in Molecular Biology, Vol 45, Iss 10, Pp 7705-7720 (2023)
CAR-T cell therapy involves genetically engineering T cells to recognize and attack tumour cells by adding a chimeric antigen receptor (CAR) to their surface. In this study, we have used dual transduction with AAV serotype 6 (AAV6) to integrate an an
Externí odkaz:
https://doaj.org/article/f4da53d5c7cf42108b22e8ca2b4715fd