Production of AFB1 High-Specificity Monoclonal Antibody by Three-Stage Screening Combined with the De-Homologation of Antibodies and the Development of High-Throughput icELISA

Autor: Chengchen Pang, Qiang Liu, Lin Chen, Bei Yuan, Chuanyun Zha, Kunying Nie, Haitao Xu, Keyun Ren, Chunlei Yu, Yemin Guo, Qingqing Yang

Publikováno v: Toxins, Vol 16, Iss 1, p 11 (2023)

To achieve accurate detection of AFB1 toxin content in agricultural products and avoid false-positive rates in the assays, the specificity of mAbs is critical. We improved the specificity of the prepared monoclonal antibodies by modifying the traditi

Externí odkaz: https://doaj.org/article/ae461ce865084f64935c7d4ea9437394

Production of AFB1 High-Specificity Monoclonal Antibody by Three-Stage Screening Combined with the De-Homologation of Antibodies and the Development of High-Throughput icELISA.

Autor: Pang C; School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun West Road, Zibo 255049, China.; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun West Road, Zibo 255049, China.; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun West Road, Zibo 255049, China., Liu Q; School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun West Road, Zibo 255049, China.; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun West Road, Zibo 255049, China.; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun West Road, Zibo 255049, China., Chen L; Hubei Provincial Institute for Food Supervision and Test, No. 8 Yaojian 2th Road, Wuhan East Lake High-Tech Development Zone, Wuhan 430075, China., Yuan B; School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun West Road, Zibo 255049, China.; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun West Road, Zibo 255049, China.; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun West Road, Zibo 255049, China., Zha C; School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun West Road, Zibo 255049, China.; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun West Road, Zibo 255049, China.; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun West Road, Zibo 255049, China., Nie K; School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun West Road, Zibo 255049, China.; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun West Road, Zibo 255049, China.; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun West Road, Zibo 255049, China., Xu H; School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun West Road, Zibo 255049, China.; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun West Road, Zibo 255049, China.; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun West Road, Zibo 255049, China., Ren K; School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun West Road, Zibo 255049, China.; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun West Road, Zibo 255049, China.; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun West Road, Zibo 255049, China., Yu C; School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun West Road, Zibo 255049, China.; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun West Road, Zibo 255049, China.; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun West Road, Zibo 255049, China., Guo Y; School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun West Road, Zibo 255049, China.; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun West Road, Zibo 255049, China.; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun West Road, Zibo 255049, China., Yang Q; School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun West Road, Zibo 255049, China.; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun West Road, Zibo 255049, China.; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun West Road, Zibo 255049, China.

Publikováno v: Toxins [Toxins (Basel)] 2023 Dec 25; Vol. 16 (1). Date of Electronic Publication: 2023 Dec 25.