Determination of Binding Affinity of Antibodies to HIV-1 Recombinant Envelope Glycoproteins, Pseudoviruses, Infectious Molecular Clones, and Cell-Expressed Trimeric gp160 Using Microscale Thermophoresis.

Autor: Basu, Shraddha^1,2 (AUTHOR) sbasu@hivresearch.org, Gohain, Neelakshi^1,2 (AUTHOR) ngohain@gmail.com, Kim, Jiae^1,2 (AUTHOR) jakim@hivresearch.org, Trinh, Hung V.^1,2 (AUTHOR) htrinh@hivresearch.org, Choe, Misook^1,3 (AUTHOR) misook.choe@nih.gov, Joyce, M. Gordon^1,3 (AUTHOR) gjoyce@eidresearch.org, Rao, Mangala² (AUTHOR) mrao@hivresearch.org

Publikováno v: Cells (2073-4409). Jan2024, Vol. 13 Issue 1, p33. 21p.

Determination of Binding Affinity of Antibodies to HIV-1 Recombinant Envelope Glycoproteins, Pseudoviruses, Infectious Molecular Clones, and Cell-Expressed Trimeric gp160 Using Microscale Thermophoresis.

Autor: Basu S; Henry M Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA.; US Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA., Gohain N; Henry M Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA.; US Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA., Kim J; Henry M Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA.; US Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA., Trinh HV; Henry M Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA.; US Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA., Choe M; Henry M Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA.; Emerging Infectious Disease Branch, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA., Joyce MG; Henry M Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA.; Emerging Infectious Disease Branch, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA., Rao M; US Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA.

Publikováno v: Cells [Cells] 2023 Dec 22; Vol. 13 (1). Date of Electronic Publication: 2023 Dec 22.

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Determination of Binding Affinity of Antibodies to HIV-1 Recombinant Envelope Glycoproteins, Pseudoviruses, Infectious Molecular Clones, and Cell-Expressed Trimeric gp160 Using Microscale Thermophoresis

Autor: Shraddha Basu, Neelakshi Gohain, Jiae Kim, Hung V. Trinh, Misook Choe, M. Gordon Joyce, Mangala Rao

Publikováno v: Cells, Vol 13, Iss 1, p 33 (2023)

Developing a preventative vaccine for HIV-1 has been a global priority. The elicitation of broadly neutralizing antibodies (bNAbs) against a broad range of HIV-1 envelopes (Envs) from various strains appears to be a critical requirement for an effica

Externí odkaz: https://doaj.org/article/94b557cde4d44f99a6f099511f0e68ac

Dendronized Gold Nanoparticles as Carriers for gp160 (HIV-1) Peptides: Biophysical Insight into Complex Formation.

Autor: Garaiova Z; Department of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University, Bratislava 842 48, Slovakia., Melikishvili S; Department of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University, Bratislava 842 48, Slovakia., Michlewska S; Laboratory of Microscopic Imaging and Specialized Biological Techniques, Faculty of Biology and Environmental Protection, University of Lodz, Lodz 90-237, Poland.; Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz 90-237, Poland., Ionov M; Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz 90-237, Poland., Pedziwiatr-Werbicka E; Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz 90-237, Poland., Waczulikova I; Department of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University, Bratislava 842 48, Slovakia., Hianik T; Department of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University, Bratislava 842 48, Slovakia., Gomez-Ramirez R; Inorganic Chemistry Department, IQAR, University Alcala, Alcala de Henares 28801, Spain.; Ramón y Cajal Health Research Institute (IRYCIS), IRYCIS, Madrid 28034, Spain.; Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid 28029, Spain., de la Mata FJ; Inorganic Chemistry Department, IQAR, University Alcala, Alcala de Henares 28801, Spain.; Ramón y Cajal Health Research Institute (IRYCIS), IRYCIS, Madrid 28034, Spain.; Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid 28029, Spain., Bryszewska M; Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz 90-237, Poland.

Publikováno v: Langmuir : the ACS journal of surfaces and colloids [Langmuir] 2021 Feb 02; Vol. 37 (4), pp. 1542-1550. Date of Electronic Publication: 2021 Jan 21.