Sample preparation methods for N-glycomics

Autor: H. Mehmet Kayili, Mehmet Atakay, Ali Hayatu, Bekir Salih

Publikováno v: Advances in Sample Preparation, Vol 4, Iss , Pp 100042- (2022)

Aberrant glycosylation has been associated with the human disease since it involves crucial mechanisms such as tumor onset, development, and progression. This review focuses on the most commonly used sample preparation methods for standard glycoprote

Externí odkaz: https://doaj.org/article/ba8de780a67d45bfb14b5336b7e6ac5b

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Thiol-ene-based microfluidic chips for glycopeptide enrichment and online digestion of inflammation-related proteins osteopontin and immunoglobulin G

Autor: Yuye Zhou, Alexander Jönsson, Drago Sticker, Guojun Zhou, Zishuo Yuan, Jörg P. Kutter, Åsa Emmer

Publikováno v: Zhou, Y, Jönsson, A, Sticker, D, Zhou, G, Yuan, Z, Kutter, J P & Emmer, Å 2023, ' Thiol-ene-based microfluidic chips for glycopeptide enrichment and online digestion of inflammation-related proteins osteopontin and immunoglobulin G ', Analytical and Bioanalytical Chemistry, vol. 415, pp. 1173–1185 . https://doi.org/10.1007/s00216-022-04498-2

Proteins, and more specifically glycoproteins, have been widely used as biomarkers, e.g., to monitor disease states. Bottom-up approaches based on mass spectrometry (MS) are techniques commonly utilized in glycoproteomics, involving protein digestion

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5bee14eb4611afdd81eeb6f835330d0d
https://doi.org/10.1007/s00216-022-04498-2