Zobrazeno 1 - 10
of 904
pro vyhledávání: '"gRNA"'
Publikováno v:
BMC Research Notes, Vol 17, Iss 1, Pp 1-6 (2024)
Abstract Objective Insertion and deletion (indel) analysis of CRISPR-Cas guide RNAs (gRNAs) is crucial in gene editing to assess gRNA efficiency and indel frequency. This study evaluates the utility of CRISPResso2 with Oxford Nanopore sequencing data
Externí odkaz:
https://doaj.org/article/be93d1b323764efb99057021330392f8
Autor:
Najmeh Heshmatpour, S. Maryam Kazemi, Niklas D. Schmidt, Sarita R. Patnaik, Patrick Korus, Bodo G. C. Wilkens, Arturo Macarrón Palacios
Publikováno v:
Frontiers in Genome Editing, Vol 6 (2024)
Diffuse large B cell lymphomas (DLBCL) are highly aggressive tumors. Their genetic complexity and heterogeneity have hampered the development of novel approaches for precision medicine. Our study aimed to develop a personalized therapy for DLBCL by u
Externí odkaz:
https://doaj.org/article/547349baf05442baab3efc48da94b2c7
Autor:
Linli Wang, Hongbing Han
Publikováno v:
Heliyon, Vol 10, Iss 19, Pp e38588- (2024)
Since its advent, gene-editing technology has been widely used in microorganisms, animals, plants, and other species. This technology shows remarkable application prospects, giving rise to a new biotechnological industry. In particular, third-generat
Externí odkaz:
https://doaj.org/article/2c424e347a1a47ccb8a494966320c9f7
Autor:
Muhammad Naveed, Fatima Tahir, Tariq Aziz, Muhammad Waseem, Syeda Izma Makhdoom, Nouman Ali, Metab Alharbi, Thamer H. Albekairi, Abdullah F. Alasmari
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-12 (2024)
Abstract Heavy metal accumulation increases rapidly in the environment due to anthropogenic activities and industrialization. The leather and surgical industry produces many contaminants containing heavy metals. Cadmium, a prominent contaminant, is l
Externí odkaz:
https://doaj.org/article/b73f4a8b51b34fbc85a991f608162c4a
Autor:
Linda Rymarquis, Chenxi Wu, Diane Hohorst, Miguel Vega‐Sanchez, Thomas E. Mullen, Vijetha Vemulapalli, Douglas R. Smith
Publikováno v:
Plant Direct, Vol 8, Iss 8, Pp n/a-n/a (2024)
Abstract Clustered regularly interspaced short palindromic repeats (CRISPR) technology has revolutionized creating targeted genetic variation in crops. Although CRISPR enzymes have been reported to have high sequence‐specificity, careful design of
Externí odkaz:
https://doaj.org/article/84920c042e24498599880637b6cbfb6a
Autor:
Gongchen Zhang, Chenyu Zhu, Xiaohan Chen, Jifang Yan, Dongyu Xue, Zixuan Wei, Guohui Chuai, Qi Liu
Publikováno v:
Genomics, Proteomics & Bioinformatics, Vol 21, Iss 6, Pp 1237-1245 (2023)
Base editing technology is being increasingly applied in genome engineering, but the current strategy for designing guide RNAs (gRNAs) relies substantially on empirical experience rather than a dependable and efficient in silico design. Furthermore,
Externí odkaz:
https://doaj.org/article/e9f9ac4390eb4669b80c2ce1bb4bcbc0
Autor:
Smitha Ijee, Karthik Chambayil, Anurag Dutta Chaudhury, Abhirup Bagchi, Kirti Modak, Saswati Das, Esther Sathya Bama Benjamin, Sonam Rani, Daniel Zechariah Paul, Aneesha Nath, Debanjan Roy, Dhavapriya Palani, Sweety Priyanka, Rakshini Ravichandran, Betty K. Kumary, Yazhini Sivamani, Vijayanand S., Dinesh Babu, Yukio Nakamura, Vasanth Thamodaran, Poonkuzhali Balasubramanian, Shaji R. Velayudhan
Publikováno v:
Frontiers in Molecular Biosciences, Vol 10 (2024)
MicroRNAs (miRNAs) are short non-coding RNAs that play crucial roles in gene regulation, exerting post-transcriptional silencing, thereby influencing cellular function, development, and disease. Traditional loss-of-function methods for studying miRNA
Externí odkaz:
https://doaj.org/article/5312775f75e844e48f5507f1e9c2a7d8
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 12 (2024)
Corynebacterium glutamicum plays a crucial role as a significant industrial producer of metabolites. Despite the successful development of CRISPR-Cas9 and CRISPR-Cas12a-assisted genome editing technologies in C. glutamicum, their editing resolution a
Externí odkaz:
https://doaj.org/article/1d2c57673d6f4bf695aa013c55f2bd4c
Autor:
Sarfaraz K. Niazi
Publikováno v:
Biologics, Vol 3, Iss 4, Pp 253-295 (2023)
Gene or genome editing (GE) revises, removes, or replaces a mutated gene at the DNA level; it is a tool. Gene therapy (GT) offsets mutations by introducing a “normal” version of the gene into the body while the diseased gene remains in the genome
Externí odkaz:
https://doaj.org/article/9a5c403e986c4d31b800a060708b6cd4
Autor:
Amira H. El-Ashry
Publikováno v:
Novel Research in Microbiology Journal, Vol 7, Iss 5, Pp 2101-2115 (2023)
The most precise, effective, and widely used tool for editing the genome is currently the clustered regularly interspaced short palindromic repeat (CRISPR), which represents the prokaryotes adaptive immune defense. The CRISPR/Cas-9 genome editing sys
Externí odkaz:
https://doaj.org/article/37e83376594549049410a928e2572952