Unambiguous identification of fungi: where do we stand and how accurate and precise is fungal DNA barcoding?

Autor: Robert Lücking, M. Catherine Aime, Barbara Robbertse, Andrew N. Miller, Hiran A. Ariyawansa, Takayuki Aoki, Gianluigi Cardinali, Pedro W. Crous, Irina S. Druzhinina, David M. Geiser, David L. Hawksworth, Kevin D. Hyde, Laszlo Irinyi, Rajesh Jeewon, Peter R. Johnston, Paul M. Kirk, Elaine Malosso, Tom W. May, Wieland Meyer, Maarja Öpik, Vincent Robert, Marc Stadler, Marco Thines, Duong Vu, Andrey M. Yurkov, Ning Zhang, Conrad L. Schoch

Publikováno v: IMA Fungus, Vol 11, Iss 1, Pp 1-32 (2020)

ABSTRACT True fungi (Fungi) and fungus-like organisms (e.g. Mycetozoa, Oomycota) constitute the second largest group of organisms based on global richness estimates, with around 3 million predicted species. Compared to plants and animals, fungi have

Externí odkaz: https://doaj.org/article/976162c8e22842139067716b573524e9

EMBL2checklists: A Python package to facilitate the user-friendly submission of plant and fungal DNA barcoding sequences to ENA.

Autor: Gruenstaeudl, Michael¹ m.gruenstaeudl@fu-berlin.de, Hartmaring, Yannick²

Publikováno v: PLoS ONE. 1/10/2019, Vol. 14 Issue 01, p1-20. 20p.

EMBL2checklists: A Python package to facilitate the user-friendly submission of plant and fungal DNA barcoding sequences to ENA.

Autor: Michael Gruenstaeudl, Yannick Hartmaring

Publikováno v: PLoS ONE, Vol 14, Iss 1, p e0210347 (2019)

BackgroundThe submission of DNA sequences to public sequence databases is an essential, but insufficiently automated step in the process of generating and disseminating novel DNA sequence data. Despite the centrality of database submissions to biolog

Externí odkaz: https://doaj.org/article/8b83c4be41f1498390c3e0bb1f9bdc2c

Preparing ONT-tagged Primers and Master Mix for Fungal DNA Barcoding v2

Autor: Stephen Douglas Russell

ONT primer preparation has two specific aspects that are unique when comparing to Sanger sequencing protocols. The first is that each primer needs to be "tagged" - a unique ~10-15bp sequence is added to the 5' end of the forward and reverse primers.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f7d6a69e1f7ec6e5013672cf55f09074
https://doi.org/10.17504/protocols.io.yxmvmnzxng3p/v2

Unambiguous identification of fungi: where do we stand and how accurate and precise is fungal DNA barcoding?

Autor: Gianluigi Cardinali, Wieland Meyer, Marc Stadler, Irina S. Druzhinina, David L. Hawksworth, Kevin D. Hyde, Maarja Öpik, Andrey Yurkov, Barbara Robbertse, Ning Zhang, Conrad L. Schoch, Marco Thines, Paul M. Kirk, Elaine Malosso, Andrew N. Miller, Takayuki Aoki, Pedro W. Crous, Hiran A. Ariyawansa, Duong Vu, Laszlo Irinyi, Tom W. May, M. Catherine Aime, David M. Geiser, Robert Lücking, Rajesh Jeewon, Vincent Robert, Peter R. Johnston

Publikováno v: IMA Fungus, Vol 11, Iss 1, Pp 1-32 (2020)
IMA fungus 11 (2020) 1
IMA Fungus, 11:14. International Mycological Association
IMA Fungus
IMA fungus
England
IMA fungus, 11(1)

True fungi (Fungi) and fungus-like organisms (e.g. Mycetozoa, Oomycota) constitute the second largest group of organisms based on global richness estimates, with around 3 million predicted species. Compared to plants and animals, fungi have simple bo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae273e094300361d1b76a6386ad6f39d
http://link.springer.com/article/10.1186/s43008-020-00033-z