Zobrazeno 1 - 10
of 321
pro vyhledávání: '"fluorescent quantitative PCR"'
Publikováno v:
Frontiers in Veterinary Science, Vol 11 (2024)
African swine fever (ASF) is a severe, hemorrhagic, and highly contagious disease caused by the African swine fever virus (ASFV) in both domestic pigs and wild boars. In China, ASFV has been present for over six years, with three genotypes of strains
Externí odkaz:
https://doaj.org/article/1e5322e7f12c45c1b50da766c042844c
Publikováno v:
Zhongguo shipin weisheng zazhi, Vol 36, Iss 2, Pp 147-155 (2024)
ObjectiveA TaqMan-based real-time fluorescence quantitative polymerase chain reaction （PCR） method was established to detect Clostridium perfringens （C. perfringens） in tap water samples.MethodsThe highly conserved plc gene located in the pse
Externí odkaz:
https://doaj.org/article/c2b6ed64e9334faca5f888fdbb0d68c3
Publikováno v:
Frontiers in Chemistry, Vol 12 (2024)
Background: Most respiratory viruses can cause serious lower respiratory diseases at any age. Therefore, timely and accurate identification of respiratory viruses has become even more important. This study focused on the development of rapid nucleic
Externí odkaz:
https://doaj.org/article/7f9c0ce6ebff4bc1856230eecdcff00e
Publikováno v:
Frontiers in Microbiology, Vol 15 (2024)
IntroductionPorcine circovirus type 2 (PCV2) is the pathogen of Porcine Circovirus Associated Diseases. Porcine circovirus type 3 (PCV3) is a novel porcine circovirus associated with porcine dermatitis and nephropathy syndrome (PDNS) and reproductive
Externí odkaz:
https://doaj.org/article/ee7e05380f8c41d5a9ed60157d3fb46d
Publikováno v:
Zhongguo gonggong weisheng, Vol 39, Iss 11, Pp 1496-1500 (2023)
ObjectiveTo establish a sensitive, reliable and rapid pre-treatment method for enrichment and nucleic acid extraction of pathogenic bacteria in water for fluorescent quantitative PCR (qPCR) detection of pathogenic bacteria in water. MethodsThe tester
Externí odkaz:
https://doaj.org/article/4cc78f45ab694fac87aba68892ac67d2
Publikováno v:
Shiyan dongwu yu bijiao yixue, Vol 43, Iss 5, Pp 566-573 (2023)
Objective To establish a method for rapid and sensitive detection of Staphylococcus aureus.Methods The specific gene nuc of Staphylococcus aureus was selected as the target gene. A pair of specific primers and a TaqMan probe were designed and synthes
Externí odkaz:
https://doaj.org/article/d35922ea0cd6477aa10ccad5f1f7f4f6
Publikováno v:
Shanghai yufang yixue, Vol 35, Iss 9, Pp 863-869 (2023)
ObjectiveTo understand the presence of virulence genes， molecular typing characteristics， and antibiotic sensitivity of enteroaggregative Escherichia coli （EAEC） in children with diarrhea in Shanghai， so as to provide a scientific basis for
Externí odkaz:
https://doaj.org/article/029e5477f50441a1a684b10e1721f912
Autor:
Wenxin Meng, Zihan Chen, Qifeng Jiang, Jinping Chen, Xiaoying Guo, Zihang Ma, Kun Jia, Shoujun Li
Publikováno v:
Frontiers in Microbiology, Vol 14 (2024)
IntroductionCalf diarrhea is a significant condition that has a strong effect on the cattle industry, resulting in huge economic losses annually. Bovine torovirus (BToV), bovine enterovirus (BEV), bovine norovirus (BNoV), bovine coronavirus (BCoV), b
Externí odkaz:
https://doaj.org/article/39e385a8559241c5a738e96bbc161e16
Publikováno v:
Respiratory Medicine Case Reports, Vol 47, Iss , Pp 101973- (2024)
Background: Real-time fluorescent quantitative PCR (RT-PCR) can effectively distinguish between Mycobacterium tuberculosis (MTB) and Non-tuberculosis mycobacterium (NTM), but when there are overlapping sequences between other pathogens (such as Nocar
Externí odkaz:
https://doaj.org/article/7e438b7999bb4200a7bd70e8f88e05e5
Autor:
Cheng Chen, Xue Zhou, Qinghong Guo, Chao Lv, Yalan Tang, Qingqing Guo, Yang Chen, Kerou Zhou, Zhiqiang Fu, Jinming Liu, Jiaojiao Lin, Yang Hong, Jun-Hu Chen
Publikováno v:
Animals, Vol 13, Iss 19, p 3068 (2023)
The prevalence of schistosomiasis japonica in China is now characterized by a low epidemic rate and low-intensity infections. Some diagnostic methods with high sensitivity and specificity are urgently needed to better monitor this disease in the curr
Externí odkaz:
https://doaj.org/article/8f75619d02db4244baaa63b8253b741f