The early history of cryo-cooling for macromolecular crystallography

Autor: David J. Haas

Publikováno v: IUCrJ, Vol 7, Iss 2, Pp 148-157 (2020)

This paper recounts the first successful cryo-cooling of protein crystals that demonstrated the reduction in X-ray damage to macromolecular crystals. The project was suggested by David C. Phillips in 1965 at the Royal Institution of Great Britain and

Externí odkaz: https://doaj.org/article/6f2dd873cbab41c0ac81f9c64b1ccb88

A miniature airlock system to aid the cryo-cooling of protein crystals grown under anoxic conditions

Autor: Peter van der Linden, Sylvain Engilberge, Mohamed Atta, Philippe Carpentier

Publikováno v: Journal of Applied Crystallography
Journal of Applied Crystallography, 2022, 55 (5), pp.1212-1218. ⟨10.1107/S1600576722007580⟩

Crystallographic studies of structures and mechanisms of dioxygen-employing enzymes or oxygen-sensitive proteins usually require these molecules to be prepared and crystallized under anoxic conditions in gloveboxes. Handling, cryo-cooling and extract

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4fc86cfbb4d517693b6f3f7c9b335626
https://doi.org/10.1107/s1600576722007580

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Methods to Screen for Radical SAM Enzyme Crystallization Conditions

Autor: Martin, Lydie, Vernède, Xavier, Nicolet, Yvain

Publikováno v: Methods in Molecular Biology
Methods in Molecular Biology, Humana Press/Springer Imprint, 2021, 2353, pp.333-348. ⟨10.1007/978-1-0716-1605-5_17⟩
Methods in Molecular Biology, 2021, 2353, pp.333-348. ⟨10.1007/978-1-0716-1605-5_17⟩

International audience; Radical S-adenosyl-L-methionine proteins most probably belong to the widest superfamily of metalloenzymes. Thanks to their ability to catalyze difficult reactions, combined with their involvement in the biosynthesis of numbers

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::32f2213534e40f8ed27e0d466f1076e9
https://pubmed.ncbi.nlm.nih.gov/34292557

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