DNA extraction protocol impacts ocular surface microbiome profile

Autor: Heleen Delbeke, Ingele Casteels, Marie Joossens

Publikováno v: Frontiers in Microbiology, Vol 14 (2023)

PurposeThe aim of this study is to provide a reference frame to allow the comparison and interpretation of currently published studies on 16S ribosomal ribonucleic acid amplicon sequencing of ocular microbiome samples using different DNA extraction p

Externí odkaz: https://doaj.org/article/6e3a77f42c0849f093be5145b7cba61b

Characterization of Ocular Surface Microbial Profiles Revealed Discrepancies between Conjunctival and Corneal Microbiota

Autor: Anna Matysiak, Michal Kabza, Justyna A. Karolak, Marcelina M. Jaworska, Malgorzata Rydzanicz, Rafal Ploski, Jacek P. Szaflik, Marzena Gajecka

Publikováno v: Pathogens, Vol 10, Iss 4, p 405 (2021)

The ocular microbiome composition has only been partially characterized. Here, we used RNA-sequencing (RNA-Seq) data to assess microbial diversity in human corneal tissue. Additionally, conjunctival swab samples were examined to characterize ocular s

Externí odkaz: https://doaj.org/article/b3636329dc434f1e926db4b8a9a4a091

The Effect of Topical Anesthetics on 16S Ribosomal Ribonucleic Acid Amplicon Sequencing Results in Ocular Surface Microbiome Research

Autor: Heleen Delbeke, Ingele Casteels, Marie Joossens

Publikováno v: TRANSLATIONAL VISION SCIENCE & TECHNOLOGY

PURPOSE: To clarify the short-term effect of topical anesthetics on 16S ribosomal ribonucleic acid amplicon sequencing results in ocular surface microbiome research. METHODS: Both eyes of 24 eligible volunteers undergoing general anesthesia were samp

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8c52b442ed008ce14fe3b6d7e2a1f142
https://lirias.kuleuven.be/handle/20.500.12942/693008

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Oxford Nanopore Technology-Based Identification of an Acanthamoeba castellanii Endosymbiosis in Microbial Keratitis.

Autor: Scharf SA; Institute of Medical Microbiology and Hospital Hygiene, Medical Faculty, Heinrich Heine University Düsseldorf, Moorenstrasse 5, 40225 Duesseldorf, Germany., Friedrichs L; Institute of Medical Microbiology and Hospital Hygiene, Medical Faculty, Heinrich Heine University Düsseldorf, Moorenstrasse 5, 40225 Duesseldorf, Germany., Bock R; Department of Ophthalmology, Medical Faculty and University Hospital Duesseldorf, Heinrich Heine University Düsseldorf, Moorenstrasse 5, 40225 Duesseldorf, Germany., Borrelli M; Department of Ophthalmology, Medical Faculty and University Hospital Duesseldorf, Heinrich Heine University Düsseldorf, Moorenstrasse 5, 40225 Duesseldorf, Germany., MacKenzie C; Institute of Medical Microbiology and Hospital Hygiene, Medical Faculty, Heinrich Heine University Düsseldorf, Moorenstrasse 5, 40225 Duesseldorf, Germany., Pfeffer K; Institute of Medical Microbiology and Hospital Hygiene, Medical Faculty, Heinrich Heine University Düsseldorf, Moorenstrasse 5, 40225 Duesseldorf, Germany., Henrich B; Institute of Medical Microbiology and Hospital Hygiene, Medical Faculty, Heinrich Heine University Düsseldorf, Moorenstrasse 5, 40225 Duesseldorf, Germany.

Publikováno v: Microorganisms [Microorganisms] 2024 Nov 12; Vol. 12 (11). Date of Electronic Publication: 2024 Nov 12.