Zobrazeno 1 - 10
of 821
pro vyhledávání: '"epigenome editing"'
Autor:
Jack B. Roberts, Olivia L.G. Boldvig, Guillaume Aubourg, S. Tanishq Kanchenapally, David J. Deehan, Sarah J. Rice, John Loughlin
Publikováno v:
Arthritis Research & Therapy, Vol 26, Iss 1, Pp 1-13 (2024)
Abstract Background Transitioning from a genetic association signal to an effector gene and a targetable molecular mechanism requires the application of functional fine-mapping tools such as reporter assays and genome editing. In this report, we unde
Externí odkaz:
https://doaj.org/article/9c31b31140bb46b0badb848d45713eab
Publikováno v:
Biochemistry and Biophysics Reports, Vol 38, Iss , Pp 101733- (2024)
RUNX2 is a transcription factor crucial for bone formation. Mutant mice with varying levels of Runx2 expression display dosage-dependent skeletal abnormalities, underscoring the importance of Runx2 dosage control in skeletal formation. RUNX2 activity
Externí odkaz:
https://doaj.org/article/2af98e2f93c14b058177a4a89c006356
Autor:
Alexandra G. Kouroukli, Nivethika Rajaram, Pavel Bashtrykov, Helene Kretzmer, Reiner Siebert, Albert Jeltsch, Susanne Bens
Publikováno v:
Clinical Epigenetics, Vol 15, Iss 1, Pp 1-15 (2023)
Abstract Background Activation of dominant oncogenes by small or structural genomic alterations is a common driver mechanism in many cancers. Silencing of such dominantly activated oncogenic alleles, thus, is a promising strategy to treat cancer. Rec
Externí odkaz:
https://doaj.org/article/11af78ae09f44988a6a8dbb6e2a1f3dd
Publikováno v:
Epigenetics & Chromatin, Vol 16, Iss 1, Pp 1-18 (2023)
Abstract Background Epigenome editing refers to the targeted reprogramming of genomic loci using an EpiEditor which may consist of an sgRNA/dCas9 complex that recruits DNMT3A/3L to the target locus. Methylation of the locus can lead to a modulation o
Externí odkaz:
https://doaj.org/article/41d1de7855f14d409bafb99c8a958394
Autor:
Arunkumar Singh Koijam, Kabrambam Dasanta Singh, Bunindro Singh Nameirakpam, Reena Haobam, Yallappa Rajashekar
Publikováno v:
Biomedicine & Pharmacotherapy, Vol 170, Iss , Pp 115951- (2024)
Drug addiction is a complex disease affected by numerous genetic and environmental factors. Brain regions in reward pathway, neuronal adaptations, genetic and epigenetic interactions causing transcriptional enhancement or repression of multiple genes
Externí odkaz:
https://doaj.org/article/8c5f6d3e0ff84c8da300647583d2394f
Publikováno v:
Frontiers in Genetics, Vol 14 (2023)
The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (Cas9) (CRISPR/Cas9) system is a powerful tool that enables precise and efficient gene manipulation. In a relatively short time, CRISPR has risen t
Externí odkaz:
https://doaj.org/article/4dcbaf1613c149f480e17c26414af005
Publikováno v:
Computational and Structural Biotechnology Journal, Vol 21, Iss , Pp 202-211 (2023)
CRISPR-mediated epigenome editing enables gene expression regulation without changing the underlying DNA sequence, and thus has vast potential for basic research and gene therapy. Effective selection of a single guide RNA (sgRNA) with high on-target
Externí odkaz:
https://doaj.org/article/1a32b1f614654ee38364a4f822e6528c
Publikováno v:
Genes, Vol 15, Iss 1, p 80 (2024)
DNA methylation is critically involved in the regulation of chromatin states and cell-type-specific gene expression. The exclusive expression of imprinted genes from either the maternal or the paternal allele is regulated by allele-specific DNA methy
Externí odkaz:
https://doaj.org/article/792651967a1243c58619181497cd55de
Autor:
Subhrangshu Guhathakurta, Levi Adams, Inhye Jeong, Anishaa Sivakumar, Mingyu Cha, Mariana Bernardo Fiadeiro, Haiyan Nancy Hu, Yoon-Seong Kim
Publikováno v:
Epigenetics, Vol 17, Iss 13, Pp 2075-2081 (2022)
Epigenetic regulation is a crucial factor controlling gene expression. Here, we report our CRISPR/dCas9-based modular epigenetic toolkit that enables gene-specific modulation of epigenetic architecture. By modifying the SunTag framework of dCas9 tagg
Externí odkaz:
https://doaj.org/article/882720c9da214885af3f9f842d677873
Publikováno v:
Epigenetics & Chromatin, Vol 15, Iss 1, Pp 1-14 (2022)
Abstract Background Epigenome-edited animal models enable direct demonstration of disease causing epigenetic mutations. Transgenic (TG) mice stably expressing epigenome-editing factors exhibit dramatic and stable changes in target epigenome modificat
Externí odkaz:
https://doaj.org/article/fa1bdf137dd141a486d26548b8f7fa38