Structure of protein O-mannose kinase reveals a unique active site architecture

Autor: Qinyu Zhu, David Venzke, Ameya S Walimbe, Mary E Anderson, Qiuyu Fu, Lisa N Kinch, Wei Wang, Xing Chen, Nick V Grishin, Niu Huang, Liping Yu, Jack E Dixon, Kevin P Campbell, Junyu Xiao

Publikováno v: eLife, Vol 5 (2016)

The ‘pseudokinase’ SgK196 is a protein O-mannose kinase (POMK) that catalyzes an essential phosphorylation step during biosynthesis of the laminin-binding glycan on α-dystroglycan. However, the catalytic mechanism underlying this activity remain

Externí odkaz: https://doaj.org/article/eec560f670404fffbe2ea90fd70ef501

Structure of protein O-mannose kinase reveals a unique active site architecture.

Autor: Zhu Q; The State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China.; Academy for Advanced Interdisciplinary Studies, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China., Venzke D; Department of Molecular Physiology and Biophysics, Howard Hughes Medical Institute, University of Iowa Roy J and Lucille A Carver College of Medicine, Iowa City, United States.; Department of Neurology, University of Iowa Roy J and Lucille A Carver College of Medicine, Iowa, United States.; Department of Internal Medicine, University of Iowa Roy J and Lucille A Carver College of Medicine, Iowa, United States., Walimbe AS; Department of Molecular Physiology and Biophysics, Howard Hughes Medical Institute, University of Iowa Roy J and Lucille A Carver College of Medicine, Iowa City, United States.; Department of Neurology, University of Iowa Roy J and Lucille A Carver College of Medicine, Iowa, United States.; Department of Internal Medicine, University of Iowa Roy J and Lucille A Carver College of Medicine, Iowa, United States., Anderson ME; Department of Molecular Physiology and Biophysics, Howard Hughes Medical Institute, University of Iowa Roy J and Lucille A Carver College of Medicine, Iowa City, United States.; Department of Neurology, University of Iowa Roy J and Lucille A Carver College of Medicine, Iowa, United States.; Department of Internal Medicine, University of Iowa Roy J and Lucille A Carver College of Medicine, Iowa, United States., Fu Q; National Institute of Biological Sciences, Beijing, China., Kinch LN; Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, United States., Wang W; Academy for Advanced Interdisciplinary Studies, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China.; Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing, China.; Synthetic and Functional Biomolecules Center, Peking University, Beijing, China.; Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University, Beijing, China., Chen X; Academy for Advanced Interdisciplinary Studies, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China.; Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing, China.; Synthetic and Functional Biomolecules Center, Peking University, Beijing, China.; Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University, Beijing, China., Grishin NV; Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, United States., Huang N; National Institute of Biological Sciences, Beijing, China., Yu L; Medical Nuclear Magnetic Resonance Facility, University of Iowa Roy J and Lucille A Carver College of Medicine, Iowa, United States., Dixon JE; Department of Pharmacology, University of California, San Diego, La Jolla, United States.; Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, United States.; Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, United States., Campbell KP; Department of Molecular Physiology and Biophysics, Howard Hughes Medical Institute, University of Iowa Roy J and Lucille A Carver College of Medicine, Iowa City, United States.; Department of Neurology, University of Iowa Roy J and Lucille A Carver College of Medicine, Iowa, United States.; Department of Internal Medicine, University of Iowa Roy J and Lucille A Carver College of Medicine, Iowa, United States., Xiao J; The State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China.; Academy for Advanced Interdisciplinary Studies, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China.

Publikováno v: ELife [Elife] 2016 Nov 23; Vol. 5. Date of Electronic Publication: 2016 Nov 23.