Zobrazeno 1 - 10
of 2 077
pro vyhledávání: '"dna isolation"'
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-10 (2024)
Abstract Although DNA extraction is a crucial step before polymerase chain reaction (PCR), attempts to perform PCR without DNA isolation have been ongoing since the 1990s. While partial success has been achieved with direct conventional PCR, the DNA
Externí odkaz:
https://doaj.org/article/2562e23647414097a92c2c342e643393
Autor:
Enrico Diniz Rodrigues Batista, Mônica Rosa Bertão, Darío Abel Palmieri, Camila Baptistão Zaniboni, Milena Cristina Moraes
Publikováno v:
Brazilian Archives of Biology and Technology, Vol 67 (2024)
Abstract Genetic analyses through molecular markers always depend on efficient protocols for DNA extraction and standardized PCR conditions. Random Amplified Polymorphic DNA (RAPD) is an inexpensive protocol that can provide information about the gen
Externí odkaz:
https://doaj.org/article/fbff0461d16746dfa860464e47f32f1a
Publikováno v:
Plant Methods, Vol 20, Iss 1, Pp 1-15 (2024)
Abstract Background There is a growing demand for fast and reliable plant biomolecular analyses. DNA extraction is the major bottleneck in plant nucleic acid-based applications especially due to the complexity of tissues in different plant species. C
Externí odkaz:
https://doaj.org/article/79b7dd4dd0a748a6b1d584962a975076
Publikováno v:
Environmental DNA, Vol 6, Iss 5, Pp n/a-n/a (2024)
ABSTRACT Ecologists, biologists, and conservation scientists are increasingly interested in the use of environmental DNA (eDNA) data for research and potentially decision‐making. While commercial DNA extraction kits are typically user‐friendly an
Externí odkaz:
https://doaj.org/article/de86be6a711e47578a888372aea8dcc4
Autor:
Sudhir Bhatia, Gudrun Baersch
Publikováno v:
Microbes and Infectious Diseases, Vol 5, Iss 1, Pp 4-10 (2024)
Aim: Many institutes have a number of old fixed slides for various pathogens. These can serve as the source of DNA reference material as well as conducting the prevalence studies of a pathogen. Moreover, such blood smeared slides can be shipped at ro
Externí odkaz:
https://doaj.org/article/518d1c6d91e347bbb462a4fc996b6f45
Publikováno v:
Медицинская иммунология, Vol 25, Iss 6, Pp 1453-1462 (2023)
Neonatal screening is a mandatory newborn screening procedure that detects the presence of genetic diseases. Dry blood stains are used for mass screening of children. This technology is the most affordable and convenient for the transportation and st
Externí odkaz:
https://doaj.org/article/824c450426fb4b8288433110b8d004fa
Autor:
Laura Guillardín, John J. MacKay
Publikováno v:
Plant Methods, Vol 19, Iss 1, Pp 1-11 (2023)
Abstract Background Genetic and genomic studies are seeing an increase in sample sizes together with a wider range of species investigated in response to environmental change concerns. In turn, these changes may come with challenges including the tim
Externí odkaz:
https://doaj.org/article/7649f547a9f14551bf24838197532b85
Publikováno v:
Nepal Journal of Biotechnology, Vol 11, Iss 2 (2023)
Coffee is an established plant for its flavor and has high commercial use. In Nepal, the popularity of coffee is increasing for its high economic value. However, its diversity and the status of its genetic mapping have not been studied in Nepal. In t
Externí odkaz:
https://doaj.org/article/b6e26785a343459fb2514053252b8021
Publikováno v:
Journal of Vector Borne Diseases, Vol 60, Iss 4, Pp 449-452 (2023)
Background & objectives: Malaria is a parasitic disease spread by Plasmodium parasite. Microscopy, lateral flow devices such as the Rapid Diagnostic Test (RDT), molecular methods such as Polymerase Chain Reaction (PCR), isothermal methods such as Loo
Externí odkaz:
https://doaj.org/article/856e3d24b417400e998be19dc01fa0bd