Detection of in vivo Protein Interactions in All Bacterial Compartments by Förster Resonance Energy Transfer with the Superfolder mTurquoise2 ox-mNeongreen FRET Pair

Autor: Meiresonne, N.Y., Consoli, E., Mertens, L.M.Y., den Blaauwen, T.

Publikováno v: BIO-PROTOCOL, 9(23):3448. Bio-protocol LLC

This protocol was developed to qualitatively and quantitatively detect protein-protein interactions in all compartments of Escherichia coli by Förster Resonance Energy Transfer (FRET) using the Superfolder mTurquoise2 ox-mNeonGreen FRET pair (sfTq2o

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::d36466af8d25e18ac700d8cbd1db81c0
https://dare.uva.nl/personal/pure/en/publications/detection-of-in-vivo-protein-interactions-in-all-bacterial-compartments-by-foerster-resonance-energy-transfer-with-the-superfolder-mturquoise2-oxmneongreen-fret-pair(1c19eec7-f2a8-4efd-80c6-3c2b19956be1).html

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Septal and lateral wall localization of PBP5, the major D,D-carboxypeptidase of Escherichia coli, requires substrate recognition and membrane attachment

Autor: Potluri, L., Karczmarek, A., Verheul, J., Piette, A., Wilkin, J.M., Werth, N., Banzhaf, M., Vollmer, W., Young, K.D., Nguyen-Distèche, M., den Blaauwen, T.

Publikováno v: Molecular Microbiology
Molecular Microbiology, 77(2), 300-323. Wiley-Blackwell

The distribution of PBP5, the major D,D-carboxypeptidase in Escherichia coli, was mapped by immunolabelling and by visualization of GFP fusion proteins in wild-type cells and in mutants lacking one or more D,D-carboxypeptidases. In addition to being

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::7cf24336cc2aa40566b7b2f43a8006fd
http://europepmc.org/articles/PMC2909392

Bacterial Cell Wall Growth, Shape and Division

Autor: Derouaux, A., Terrak, M., den Blaauwen, T., Vollmer, W., Remaut, H., Fronzes, R.

Publikováno v: Bacterial membranes: structural and molecular biology, 3-54
STARTPAGE=3;ENDPAGE=54;TITLE=Bacterial membranes: structural and molecular biology

The shape of a bacterial cell is maintained by its peptidoglycan sacculus that completely surrounds the cytoplasmic membrane. During growth the sacculus is enlarged by peptidoglycan synthesis complexes that are controlled by components linked to the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=narcis______::4d0f5c78a511b0120b9545f1cd61d622
https://dare.uva.nl/personal/pure/en/publications/bacterial-cell-wall-growth-shape-and-division(6f2dc821-6806-4968-bc7a-fb32c072ee50).html

In vivo bacterial morphogenetic protein interactions

Autor: van der Ploeg, R., den Blaauwen, T., Meghea, A.

Publikováno v: Molecular Interactions
Molecular interactions, 229-262
STARTPAGE=229;ENDPAGE=262;TITLE=Molecular interactions

This chapter will discuss none-invasive techniques that are widely used to study protein-protein interactions. As an example, their application in exploring interactions between proteins involved in bacterial cell division will be evaluated. First, b

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c9bfe2de8bdcf57052745ad9a08f337b
https://doi.org/10.5772/36552