Structured Illumination Microscopy Improves Spot Detection Performance in Spatial Transcriptomics

Autor: Alejandro Linares, Carlo Brighi, Sergio Espinola, Francesco Bacchi, Álvaro H. Crevenna

Publikováno v: Cells, Vol 12, Iss 9, p 1310 (2023)

Spatial biology is a rapidly growing research field that focuses on the transcriptomic or proteomic profiling of single cells within tissues with preserved spatial information. Imaging-based spatial transcriptomics uses epifluorescence microscopy, wh

Externí odkaz: https://doaj.org/article/e041a4b7548f4fe193e0c14516b48034

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Comparing super-resolution microscopy techniques to analyze chromosomes

Autor: Eva Hřibová, Alžběta Němečková, Veit Schubert, Ivona Kubalová, Klaus Weisshart

Publikováno v: International journal of molecular sciences, 22(4):1903
International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 22, Iss 1903, p 1903 (2021)
Volume 22
Issue 4

The importance of fluorescence light microscopy for understanding cellular and sub-cellular structures and functions is undeniable. However, the resolution is limited by light diffraction (~200–250 nm laterally, ~500–700 nm axially). Meanwhile, s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::49e26371f78e3a09b36d5933ed1efbb8
https://www.mdpi.com/1422-0067/22/4/1903/s1

Nanoscopic Visualization of Restricted Nonvolume Cholinergic and Monoaminergic Transmission with Genetically Encoded Sensors

Autor: Philip M. Borden, Kaiming Guo, Miao Jing, Jonathan S. Marvin, Jinxia Wan, Li Lin, Yajun Zhang, W Sharon Zheng, Yulong Li, Paula K Zhu, Loren L. Looger, Jiesi Feng, Yali Wang, Peng Zhang, Alex C. Kwan, Farhan Ali, Li Gan

Publikováno v: Nano letters, vol 20, iss 6
Nano Lett

How neuromodulatory transmitters diffuse into the extracellular space remains an unsolved fundamental biological question, despite wide acceptance of the volume transmission model. Here, we report development of a method combining genetically encoded

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5e2de0f1297a9ca14528bd4ff238c3e0
https://escholarship.org/uc/item/5bk760zc

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