Unbiased method for spectral analysis of cells with great diversity of autofluorescence spectra.

Autor: Roet JEG; Department of Molecular Cell Biology and Immunology, Amsterdam UMC location Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.; Amsterdam Institute for Immunology and Infectious Diseases, Amsterdam, The Netherlands., Mikula AM; Department of Molecular Cell Biology and Immunology, Amsterdam UMC location Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.; Amsterdam Institute for Immunology and Infectious Diseases, Amsterdam, The Netherlands., de Kok M; Department of Molecular Cell Biology and Immunology, Amsterdam UMC location Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.; Amsterdam Institute for Immunology and Infectious Diseases, Amsterdam, The Netherlands.; Microscopy and Cytometry Core Facility, Amsterdam UMC location Vrije Universiteit Amsterdam, Amsterdam, The Netherlands., Chadick CH; Department of Molecular Cell Biology and Immunology, Amsterdam UMC location Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.; Amsterdam Institute for Immunology and Infectious Diseases, Amsterdam, The Netherlands.; Microscopy and Cytometry Core Facility, Amsterdam UMC location Vrije Universiteit Amsterdam, Amsterdam, The Netherlands., Garcia Vallejo JJ; Department of Molecular Cell Biology and Immunology, Amsterdam UMC location Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.; Amsterdam Institute for Immunology and Infectious Diseases, Amsterdam, The Netherlands.; Microscopy and Cytometry Core Facility, Amsterdam UMC location Vrije Universiteit Amsterdam, Amsterdam, The Netherlands., Roest HP; Department of Surgery, Erasmus MC Transplant Institute, University Medical Center Rotterdam, Rotterdam, The Netherlands., van der Laan LJW; Department of Surgery, Erasmus MC Transplant Institute, University Medical Center Rotterdam, Rotterdam, The Netherlands., de Winde CM; Department of Molecular Cell Biology and Immunology, Amsterdam UMC location Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.; Amsterdam Institute for Immunology and Infectious Diseases, Amsterdam, The Netherlands.; Cancer Biology and Immunology, Cancer Center Amsterdam, Amsterdam, The Netherlands., Mebius RE; Department of Molecular Cell Biology and Immunology, Amsterdam UMC location Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.; Amsterdam Institute for Immunology and Infectious Diseases, Amsterdam, The Netherlands.

Publikováno v: Cytometry. Part A : the journal of the International Society for Analytical Cytology [Cytometry A] 2024 Aug; Vol. 105 (8), pp. 595-606. Date of Electronic Publication: 2024 Jun 12.

Pancreatic cancer-associated fibroblasts modulate macrophage differentiation via sialic acid-Siglec interactions.

Autor: Boelaars K; Amsterdam UMC location Vrije Universiteit Amsterdam, Molecular Cell Biology and Immunology, De Boelelaan, 1117, Amsterdam, Netherlands.; Cancer Center Amsterdam, Cancer Biology and Immunology, Amsterdam, The Netherlands.; Amsterdam Institute for Infection and Immunity, Cancer Immunology, Amsterdam, The Netherlands., Rodriguez E; Amsterdam UMC location Vrije Universiteit Amsterdam, Molecular Cell Biology and Immunology, De Boelelaan, 1117, Amsterdam, Netherlands.; Cancer Center Amsterdam, Cancer Biology and Immunology, Amsterdam, The Netherlands.; Amsterdam Institute for Infection and Immunity, Cancer Immunology, Amsterdam, The Netherlands., Huinen ZR; Amsterdam UMC location Vrije Universiteit Amsterdam, Molecular Cell Biology and Immunology, De Boelelaan, 1117, Amsterdam, Netherlands.; Cancer Center Amsterdam, Cancer Biology and Immunology, Amsterdam, The Netherlands.; Amsterdam Institute for Infection and Immunity, Cancer Immunology, Amsterdam, The Netherlands., Liu C; Amsterdam UMC location Vrije Universiteit Amsterdam, Molecular Cell Biology and Immunology, De Boelelaan, 1117, Amsterdam, Netherlands.; Cancer Center Amsterdam, Cancer Biology and Immunology, Amsterdam, The Netherlands.; Amsterdam Institute for Infection and Immunity, Cancer Immunology, Amsterdam, The Netherlands.; Amsterdam UMC location Vrije Universiteit Amsterdam, Pulmonary Medicine, De Boelelaan, 1117, Amsterdam, the Netherlands., Wang D; Leiden University Medical Center, Center for Proteomics and Metabolomics, Albinusdreef 2, 2333 ZA, Leiden, the Netherlands., Springer BO; Amsterdam UMC location Vrije Universiteit Amsterdam, Molecular Cell Biology and Immunology, De Boelelaan, 1117, Amsterdam, Netherlands.; Cancer Center Amsterdam, Cancer Biology and Immunology, Amsterdam, The Netherlands.; Amsterdam Institute for Infection and Immunity, Cancer Immunology, Amsterdam, The Netherlands., Olesek K; Amsterdam UMC location Vrije Universiteit Amsterdam, Molecular Cell Biology and Immunology, De Boelelaan, 1117, Amsterdam, Netherlands.; Cancer Center Amsterdam, Cancer Biology and Immunology, Amsterdam, The Netherlands.; Amsterdam Institute for Infection and Immunity, Cancer Immunology, Amsterdam, The Netherlands., Goossens-Kruijssen L; Amsterdam UMC location Vrije Universiteit Amsterdam, Molecular Cell Biology and Immunology, De Boelelaan, 1117, Amsterdam, Netherlands.; Cancer Center Amsterdam, Cancer Biology and Immunology, Amsterdam, The Netherlands.; Amsterdam Institute for Infection and Immunity, Cancer Immunology, Amsterdam, The Netherlands., van Ee T; Amsterdam UMC location Vrije Universiteit Amsterdam, Molecular Cell Biology and Immunology, De Boelelaan, 1117, Amsterdam, Netherlands.; Cancer Center Amsterdam, Cancer Biology and Immunology, Amsterdam, The Netherlands.; Amsterdam Institute for Infection and Immunity, Cancer Immunology, Amsterdam, The Netherlands., Lindijer D; Amsterdam UMC location Vrije Universiteit Amsterdam, Molecular Cell Biology and Immunology, De Boelelaan, 1117, Amsterdam, Netherlands.; Cancer Center Amsterdam, Cancer Biology and Immunology, Amsterdam, The Netherlands.; Amsterdam Institute for Infection and Immunity, Cancer Immunology, Amsterdam, The Netherlands., Tak W; Amsterdam UMC location Vrije Universiteit Amsterdam, Molecular Cell Biology and Immunology, De Boelelaan, 1117, Amsterdam, Netherlands.; Cancer Center Amsterdam, Cancer Biology and Immunology, Amsterdam, The Netherlands.; Amsterdam Institute for Infection and Immunity, Cancer Immunology, Amsterdam, The Netherlands., de Haas A; Amsterdam UMC location Vrije Universiteit Amsterdam, Molecular Cell Biology and Immunology, De Boelelaan, 1117, Amsterdam, Netherlands.; Cancer Center Amsterdam, Cancer Biology and Immunology, Amsterdam, The Netherlands.; Amsterdam Institute for Infection and Immunity, Cancer Immunology, Amsterdam, The Netherlands., Wehry L; Amsterdam UMC location Vrije Universiteit Amsterdam, Molecular Cell Biology and Immunology, De Boelelaan, 1117, Amsterdam, Netherlands.; Cancer Center Amsterdam, Cancer Biology and Immunology, Amsterdam, The Netherlands.; Amsterdam Institute for Infection and Immunity, Cancer Immunology, Amsterdam, The Netherlands., Nugteren-Boogaard JP; Amsterdam UMC location Vrije Universiteit Amsterdam, Molecular Cell Biology and Immunology, De Boelelaan, 1117, Amsterdam, Netherlands.; Amsterdam Institute for Infection and Immunity, Cancer Immunology, Amsterdam, The Netherlands., Mikula A; Amsterdam UMC location Vrije Universiteit Amsterdam, Molecular Cell Biology and Immunology, De Boelelaan, 1117, Amsterdam, Netherlands.; Cancer Center Amsterdam, Cancer Biology and Immunology, Amsterdam, The Netherlands.; Amsterdam Institute for Infection and Immunity, Cancer Immunology, Amsterdam, The Netherlands., de Winde CM; Amsterdam UMC location Vrije Universiteit Amsterdam, Molecular Cell Biology and Immunology, De Boelelaan, 1117, Amsterdam, Netherlands.; Cancer Center Amsterdam, Cancer Biology and Immunology, Amsterdam, The Netherlands.; Amsterdam Institute for Infection and Immunity, Cancer Immunology, Amsterdam, The Netherlands., Mebius RE; Amsterdam UMC location Vrije Universiteit Amsterdam, Molecular Cell Biology and Immunology, De Boelelaan, 1117, Amsterdam, Netherlands.; Cancer Center Amsterdam, Cancer Biology and Immunology, Amsterdam, The Netherlands.; Amsterdam Institute for Infection and Immunity, Cancer Immunology, Amsterdam, The Netherlands., Tuveson DA; Laboratory, Cold Spring Harbor, New York, USA., Giovannetti E; Cancer Center Amsterdam, Cancer Biology and Immunology, Amsterdam, The Netherlands.; Amsterdam UMC location Vrije Universiteit Amsterdam, Medical Oncology, De Boelelaan, 1117, Amsterdam, Netherlands.; Cancer Pharmacology Lab, AIRC Start-Up Unit, Fondazione Pisana per la Scienza, Pisa, Italy., Bijlsma MF; Cancer Center Amsterdam, Cancer Biology and Immunology, Amsterdam, The Netherlands.; Amsterdam UMC, location University of Amsterdam, Center for Experimental and Molecular Medicine, Laboratory for Experimental Oncology and Radiobiology, Meibergdreef 9, 1105AZ, Amsterdam, The Netherlands.; Oncode Institute, Amsterdam, The Netherlands., Wuhrer M; Leiden University Medical Center, Center for Proteomics and Metabolomics, Albinusdreef 2, 2333 ZA, Leiden, the Netherlands., van Vliet SJ; Amsterdam UMC location Vrije Universiteit Amsterdam, Molecular Cell Biology and Immunology, De Boelelaan, 1117, Amsterdam, Netherlands.; Cancer Center Amsterdam, Cancer Biology and Immunology, Amsterdam, The Netherlands.; Amsterdam Institute for Infection and Immunity, Cancer Immunology, Amsterdam, The Netherlands., van Kooyk Y; Amsterdam UMC location Vrije Universiteit Amsterdam, Molecular Cell Biology and Immunology, De Boelelaan, 1117, Amsterdam, Netherlands. y.vankooyk@amsterdamumc.nl.; Cancer Center Amsterdam, Cancer Biology and Immunology, Amsterdam, The Netherlands. y.vankooyk@amsterdamumc.nl.; Amsterdam Institute for Infection and Immunity, Cancer Immunology, Amsterdam, The Netherlands. y.vankooyk@amsterdamumc.nl.

Publikováno v: Communications biology [Commun Biol] 2024 Apr 09; Vol. 7 (1), pp. 430. Date of Electronic Publication: 2024 Apr 09.

Cancer-on-chip models for metastasis: importance of the tumor microenvironment.

Autor: Jouybar M; Microsystems, Eindhoven University of Technology, Eindhoven, The Netherlands; Institute for Complex Molecular Systems, Eindhoven, The Netherlands., de Winde CM; Amsterdam UMC location Vrije Universiteit Amsterdam, Molecular Cell Biology & Immunology, Amsterdam, The Netherlands; Amsterdam Institute for Infection and Immunity, Cancer Immunology, Amsterdam, The Netherlands; Cancer Center Amsterdam, Cancer Biology & Immunology, Amsterdam, The Netherlands., Wolf K; Department of Medical BioSciences, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, The Netherlands., Friedl P; Department of Medical BioSciences, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, The Netherlands; Department of Genitourinary Medical Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA; Cancer Genomics Center, Utrecht, The Netherlands., Mebius RE; Amsterdam UMC location Vrije Universiteit Amsterdam, Molecular Cell Biology & Immunology, Amsterdam, The Netherlands; Amsterdam Institute for Infection and Immunity, Cancer Immunology, Amsterdam, The Netherlands; Cancer Center Amsterdam, Cancer Biology & Immunology, Amsterdam, The Netherlands; Amsterdam Institute for Infection and Immunity, Inflammatory diseases, Amsterdam, The Netherlands., den Toonder JMJ; Microsystems, Eindhoven University of Technology, Eindhoven, The Netherlands; Institute for Complex Molecular Systems, Eindhoven, The Netherlands. Electronic address: J.M.J.d.Toonder@tue.nl.

Publikováno v: Trends in biotechnology [Trends Biotechnol] 2024 Apr; Vol. 42 (4), pp. 431-448. Date of Electronic Publication: 2023 Oct 31.

Quantitative single molecule analysis of podoplanin clustering in fibroblastic reticular cells uncovers CD44 function.

Autor: Lim SE; Stromal Immunology Group, MRC Laboratory for Molecular Cell Biology, University College London, Gower Street, London WC1E 6BT, UK.; London Centre for Nanotechnology, University College London, London WC1H 0AH, UK., Joseph MD; London Centre for Nanotechnology, University College London, London WC1H 0AH, UK.; Department of Chemistry, University College London, London WC1H 0AJ, UK., de Winde CM; Stromal Immunology Group, MRC Laboratory for Molecular Cell Biology, University College London, Gower Street, London WC1E 6BT, UK.; Department of Molecular Cell Biology & Immunology, Amsterdam UMC location Vrije Universiteit Amsterdam, De Boelelaan 1117, Amsterdam, The Netherlands.; Cancer Center Amsterdam, Cancer Biology & Immunology, Amsterdam, The Netherlands.; Amsterdam Institute for Infection & Immunity, Cancer Immunology, Amsterdam, The Netherlands., Acton SE; Stromal Immunology Group, MRC Laboratory for Molecular Cell Biology, University College London, Gower Street, London WC1E 6BT, UK., Simoncelli S; London Centre for Nanotechnology, University College London, London WC1H 0AH, UK.; Department of Chemistry, University College London, London WC1H 0AJ, UK.

Publikováno v: Open biology [Open Biol] 2023 May; Vol. 13 (5), pp. 220377. Date of Electronic Publication: 2023 May 10.

Recommendations for empowering early career researchers to improve research culture and practice.

Autor: Kent BA; Department of Psychology, Simon Fraser University, Burnaby, Canada., Holman C; BIH QUEST Center for Responsible Research, Berlin Institute of Health at Charité-Universitätsmedizin Berlin, Berlin, Germany., Amoako E; Department of Paediatrics and Child Health, Cape Coast Teaching Hospital, Cape Coast, Ghana.; Department of Paediatrics and Child Health, School of Medicine, University of Cape Coast, Cape Coast, Ghana., Antonietti A; Department of Electronics, Information and Bioengineering, Politecnico di Milano, Milano, Italy., Azam JM; Department of Mathematics, DSI-NRF Center of Excellence in Epidemiological Modelling and Analysis, Stellenbosch University, Stellenbosch, South Africa., Ballhausen H; BIH QUEST Center for Responsible Research, Berlin Institute of Health at Charité-Universitätsmedizin Berlin, Berlin, Germany.; Department of Paediatric Endocrinology and Diabetes, Charité-Universitätsmedizin Berlin, Berlin, Germany., Bediako Y; West African Centre for Cell Biology of Infectious Pathogens, University of Ghana, Accra, Ghana., Belasen AM; Department of Ecology and Evolutionary Biology, Cornell University, Ithaca, New York, United States of America.; Society for Conservation Biology, Washington, DC, United States of America., Carneiro CFD; Institute of Medical Biochemistry Leopoldo de Meis, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Chen YC; Department of Biology, New York University, New York, New York, United States of America., Compeer EB; Kennedy Institute of Rheumatology, University of Oxford, Oxford, United Kingdom., Connor CAC; Clemson University, Clemson, South Carolina, United States of America., Crüwell S; BIH QUEST Center for Responsible Research, Berlin Institute of Health at Charité-Universitätsmedizin Berlin, Berlin, Germany., Debat H; Center of Agronomic Research, National Institute of Agricultural Technology (IPAVE-CIAP-INTA), Buenos Aires, Argentina., Dorris E; Conway Institute for Biomolecular and Biomedical Research, School of Medicine, University College Dublin, Dublin, Ireland., Ebrahimi H; Non-Communicable Diseases Research Center, Endocrinology and Metabolism Population Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran., Erlich JC; New York University Shanghai, Shanghai, China.; Sainsbury Wellcome Centre, University College London, London, United Kingdom., Fernández-Chiappe F; Instituto de Investigación en Biomedicina de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Partner Institute of the Max Planck Society, Buenos Aires, Argentina., Fischer F; Department of Psychosomatic Medicine, Center for Internal Medicine and Dermatology, Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany., Gazda MA; Département de Biologie, École Normale Supérieure, CNRS, Institut de Biologie de l'ENS (IBENS), INSERM, Université PSL, Paris, France., Glatz T; Institute of Public Health, Charité-Universitätsmedizin Berlin, Berlin, Germany., Grabitz P; BIH QUEST Center for Responsible Research, Berlin Institute of Health at Charité-Universitätsmedizin Berlin, Berlin, Germany., Heise V; Hanse-Wissenschaftskolleg (Institute for Advanced Study), Delmenhorst, Germany., Kent DG; York Biomedical Research Institute, Department of Biology, University of York, York, United Kingdom., Lo H; Neuroscience Research Center, Charité-Universitätsmedizin Berlin, Germany.; Einstein Center for Neurosciences, Berlin, Germany., McDowell G; Lightoller LLC, Chicago Illinois, United States of America., Mehta D; Department of Biological Sciences, University of Alberta, Edmonton, Canada., Neumann WJ; Movement Disorder and Neuromodulation Unit, Department of Neurology, Charité-Universitätsmedizin Berlin, Berlin, Germany., Neves K; Institute of Medical Biochemistry Leopoldo de Meis, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Patterson M; Retired, Cambridge, United Kingdom., Penfold NC; eLife (formerly), Cambridge, United Kingdom., Piper SK; Institute of Biometry and Clinical Epidemiology, Charité-Universitätsmedizin Berlin, Berlin, Germany.; Berlin Institute of Health (BIH), Berlin, Germany., Puebla I; ASAPbio, Cambridge, United Kingdom., Quashie PK; West African Centre for Cell Biology of Infectious Pathogens, University of Ghana, Accra, Ghana.; Francis Crick Institute, London, United Kingdom., Quezada CP; Departamento de Química Ambiental, Facultad de Ciencias, Universidad Católica de la Santísima Concepción, Concepción, Chile., Riley JL; Department of Biology, Mount Allison University, New Brunswick, Canada., Rohmann JL; Institute of Public Health, Charité-Universitätsmedizin Berlin, Berlin, Germany.; Center for Stroke Research, Charité-Universitätsmedizin Berlin, Berlin, Germany., Saladi S; California Institute of Technology, Pasedena, California, United States of America., Schwessinger B; Research School of Biology, The Australian National University, Canberra, Australia., Siegerink B; Department of Clinical Epidemiology, Leiden University Medical Center, Leiden, the Netherlands.; Directorate of Research Policy, Leiden University Medical Center, Leiden, the Netherlands., Stehlik P; Institute for Evidence-Based Healthcare, Bond University, Robina, Australia.; Evidence-Based Practice Professorial Unit, Gold Coast Hospital and Health Service, Southport, Australia., Tzilivaki A; Neuroscience Research Center, Charité-Universitätsmedizin Berlin, Germany.; Einstein Center for Neurosciences, Berlin, Germany.; NeuroCure Cluster of Excellence Berlin, Germany., Umbers KDL; School of Science, Western Sydney University, Penrith, Australia., Varma A; National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India., Walavalkar K; National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India., de Winde CM; Department of Molecular Cell Biology and Immunology, Amsterdam UMC location VU, Amsterdam, the Netherlands., Zaza C; Centro de Investigaciones en Bionanociencias (CIBION), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina., Weissgerber TL; BIH QUEST Center for Responsible Research, Berlin Institute of Health at Charité-Universitätsmedizin Berlin, Berlin, Germany.

Publikováno v: PLoS biology [PLoS Biol] 2022 Jul 07; Vol. 20 (7), pp. e3001680. Date of Electronic Publication: 2022 Jul 07 (Print Publication: 2022).