Development and evaluation of a single-tube nested PCR with colorimetric assay for Mycobacterium tuberculosis detection.

Autor: Salazar MP; Laboratory of Immunoepidemiology, Department of Immunology, Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Pernambuco, 50740-465, Brazil.; Contributed equally to this work and are considered as co-first authors., da Costa Lima Suassuna Monteiro JF; Laboratory of Immunoepidemiology, Department of Immunology, Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Pernambuco, 50740-465, Brazil.; Contributed equally to this work and are considered as co-first authors., Veloso Carvalho-Silva WH; Laboratory of Immunoepidemiology, Department of Immunology, Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Pernambuco, 50740-465, Brazil., Nunes Diniz GT; Laboratory of Quantitative Methods, Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Pernambuco, 50740-465, Brazil., Werkhauser RP; Laboratory of Immunoepidemiology, Department of Immunology, Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Pernambuco, 50740-465, Brazil., Lapa Montenegro LM; Laboratory of Immunoepidemiology, Department of Immunology, Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Pernambuco, 50740-465, Brazil., Schindler HC; Laboratory of Immunoepidemiology, Department of Immunology, Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Pernambuco, 50740-465, Brazil.

Publikováno v: BioTechniques [Biotechniques] 2024; Vol. 76 (6), pp. 235-244. Date of Electronic Publication: 2024 Apr 11.