Zobrazeno 1 - 10
of 13 729
pro vyhledávání: '"cryo‐EM"'
Publikováno v:
Prion, Vol 18, Iss 1, Pp 28-39 (2024)
ABSTRACTHeterogeneous nuclear ribonucleoproteins (hnRNPs) are multifunctional proteins with integral roles in RNA metabolism and the regulation of alternative splicing. These proteins typically contain prion-like domains of low complexity (PrLDs or L
Externí odkaz:
https://doaj.org/article/5a296812dab845b192abfe0a4c9b32c0
Publikováno v:
IUCrJ, Vol 11, Iss 6, Pp 951-965 (2024)
Conformational heterogeneity of biological macromolecules is a challenge in single-particle averaging (SPA). Current standard practice is to employ classification and filtering methods that may allow a discrete number of conformational states to be r
Externí odkaz:
https://doaj.org/article/6b34ad7c49d04d6f9fe41fef2b110fc4
Publikováno v:
IUCrJ, Vol 11, Iss 6, Pp 921-937 (2024)
X-ray and neutron crystallography, as well as cryogenic electron microscopy (cryo-EM), are the most common methods to obtain atomic structures of biological macromolecules. A feature they all have in common is that, at typical resolutions, the experi
Externí odkaz:
https://doaj.org/article/ed3d84f15d0d493d96cc34cddfc9f408
Autor:
Rajani Kanth Gudipati, Dimos Gaidatzis, Jan Seebacher, Sandra Muehlhaeusser, Georg Kempf, Simone Cavadini, Daniel Hess, Charlotte Soneson, Helge Großhans
Publikováno v:
Molecular Systems Biology, Vol 20, Iss 12, Pp 1303-1328 (2024)
Abstract Substrate specificity determines protease functions in physiology and in clinical and biotechnological applications, yet quantitative cleavage information is often unavailable, biased, or limited to a small number of events. Here, we develop
Externí odkaz:
https://doaj.org/article/90d17309994f44198ecc68e8695d20d8
Autor:
Yinglong Miao, Michael S. Wolfe
Publikováno v:
Neural Regeneration Research, Vol 20, Iss 1, Pp 174-180 (2025)
γ-Secretase, called “the proteasome of the membrane,” is a membrane-embedded protease complex that cleaves 150+ peptide substrates with central roles in biology and medicine, including amyloid precursor protein and the Notch family of cell-surfa
Externí odkaz:
https://doaj.org/article/70ecd82e09e2493cb7af8e0fad15ae36
Publikováno v:
IUCrJ, Vol 11, Iss 5, Pp 723-729 (2024)
Single-particle cryo-electron microscopy (cryo-EM) has become an essential structural determination technique with recent hardware developments making it possible to reach atomic resolution, at which individual atoms, including hydrogen atoms, can be
Externí odkaz:
https://doaj.org/article/274a233e1353469aa8ae96e18a562693
Publikováno v:
IUCrJ, Vol 11, Iss 5, Pp 871-877 (2024)
Identifying and characterizing metal-binding sites (MBS) within macromolecular structures is imperative for elucidating their biological functions. CheckMyMetal (CMM) is a web based tool that facilitates the interactive validation of MBS in structure
Externí odkaz:
https://doaj.org/article/9b1faae132454f2b898dab5374e35ffd
Publikováno v:
IUCrJ, Vol 11, Iss 5, Pp 821-830 (2024)
Cryogenic electron microscopy (cryo-EM) is a pivotal technique for imaging macromolecular structures. However, despite extensive processing of large image sets collected in cryo-EM experiments to amplify the signal-to-noise ratio, the reconstructed 3
Externí odkaz:
https://doaj.org/article/a1444644ec6a402c8109bdd907d0a9e2
Publikováno v:
Applied Microscopy, Vol 54, Iss 1, Pp 1-6 (2024)
Abstract The sliding filament theory and the cross-bridge model have been fundamental in understanding muscle contraction. While the cross-bridge model explains the interaction between a single myosin head and actin filament, the native myosin molecu
Externí odkaz:
https://doaj.org/article/8e03baa00f944317872fe6b00c7b1acc
Autor:
Tomasz Włodarski, Julian O. Streit, Alkistis Mitropoulou, Lisa D. Cabrita, Michele Vendruscolo, John Christodoulou
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-16 (2024)
Abstract Cryogenic electron microscopy (cryo-EM) has emerged as a powerful method for the determination of structures of complex biological molecules. The accurate characterisation of the dynamics of such systems, however, remains a challenge. To add
Externí odkaz:
https://doaj.org/article/34880de7ddfb434da7db67f9fc377c9f