Zobrazeno 1 - 10
of 1 365
pro vyhledávání: '"crispr interference"'
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-13 (2024)
Abstract CRISPR-dead Cas9 interference (CRISPRi) has become a valuable tool for precise gene regulation. In this study, CRISPRi was designed to target the inhA gene of Mycobacterium smegmatis (Msm), a gene necessary for mycolic acid synthesis. Our fi
Externí odkaz:
https://doaj.org/article/59b9b29f6fe844e7b45659bffe016362
Publikováno v:
Microbial Cell Factories, Vol 23, Iss 1, Pp 1-17 (2024)
Abstract Background The type II based CRISPR-Cas system remains restrictedly utilized in archaea, a featured domain of life that ranks parallelly with Bacteria and Eukaryotes. Methanococcus maripaludis, known for rapid growth and genetic tractability
Externí odkaz:
https://doaj.org/article/fcd13bc3f0504c82bb4f39c2ec9afa2e
Publikováno v:
Jichu yixue yu linchuang, Vol 44, Iss 6, Pp 821-827 (2024)
Objective To explore the design of CRISPR interference (CRISPRi) tools based on the deactivated CasMINI (dCasMINI) protein and to evaluate their transcriptional inhibition effects. Methods The tetracycline-on (tet-on) system, flow cytometry, and quan
Externí odkaz:
https://doaj.org/article/c618e3ee9e5f456a8a160ea9982173c3
Autor:
Xue Liu, Vincent de Bakker, Maria Victoria Heggenhougen, Marita Torrissen Mårli, Anette Heidal Frøynes, Zhian Salehian, Davide Porcellato, Danae Morales Angeles, Jan-Willem Veening, Morten Kjos
Publikováno v:
mSystems, Vol 9, Iss 7 (2024)
ABSTRACT Antibiotic resistance and tolerance remain a major problem for the treatment of staphylococcal infections. Identifying genes that influence antibiotic susceptibility could open the door to novel antimicrobial strategies, including targets fo
Externí odkaz:
https://doaj.org/article/e408a377f78a4ede9c4a628939f6f6e4
Autor:
Gautam K. Pandey, Swarooparani Vadlamudi, Kevin W. Currin, Anne H. Moxley, Jayna C. Nicholas, Jessica C. McAfee, K. Alaine Broadaway, Karen L. Mohlke
Publikováno v:
HGG Advances, Vol 5, Iss 2, Pp 100275- (2024)
Summary: Genome-wide association studies (GWASs) have identified hundreds of risk loci for liver disease and lipid-related metabolic traits, although identifying their target genes and molecular mechanisms remains challenging. We predicted target gen
Externí odkaz:
https://doaj.org/article/8b7139b6dc3b4818bc6f4371df2c9d29
Autor:
Alina Guna, Katharine R. Page, Joseph M. Replogle, Theodore K. Esantsi, Maxine L. Wang, Jonathan S. Weissman, Rebecca M. Voorhees
Publikováno v:
BMC Genomics, Vol 24, Iss 1, Pp 1-13 (2023)
Abstract Mapping genetic interactions is essential for determining gene function and defining novel biological pathways. We report a simple to use CRISPR interference (CRISPRi) based platform, compatible with Fluorescence Activated Cell Sorting (FACS
Externí odkaz:
https://doaj.org/article/595c5032f9dd4a8ca48b4d5775c55fa0
Autor:
Ryan D. Ward, Jennifer S. Tran, Amy B. Banta, Emily E. Bacon, Warren E. Rose, Jason M. Peters
Publikováno v:
mBio, Vol 15, Iss 2 (2024)
ABSTRACT The emergence of multidrug-resistant Gram-negative bacteria underscores the need to define genetic vulnerabilities that can be therapeutically exploited. The Gram-negative pathogen, Acinetobacter baumannii, is considered an urgent threat due
Externí odkaz:
https://doaj.org/article/7edba44f48f74278b241d57f3bc36657
Autor:
Jinho Kim, Taek Soon Lee
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 11 (2023)
Regulation of metabolic gene expression is crucial for maximizing bioproduction titers. Recent engineering tools including CRISPR/Cas9, CRISPR interference (CRISPRi), and CRISPR activation (CRISPRa) have enabled effective knock-out, knock-down, and o
Externí odkaz:
https://doaj.org/article/9f8e6a551be849b8a72d544745a73d44
Publikováno v:
Engineering Microbiology, Vol 3, Iss 3, Pp 100089- (2023)
Deciphering gene function is fundamental to engineering of microbiology. The clustered regularly interspaced short palindromic repeats (CRISPR) system has been adapted for gene repression across a range of hosts, creating a versatile tool called CRIS
Externí odkaz:
https://doaj.org/article/a732739b1ded4cb7b6275891f3502a33
Autor:
Sarah Pickles, Desiree Zanetti Alepuz, Yuka Koike, Mei Yue, Jimei Tong, Pinghu Liu, Yugui Zhou, Karen Jansen-West, Lillian M. Daughrity, Yuping Song, Michael DeTure, Björn Oskarsson, Neill R. Graff-Radford, Bradley F. Boeve, Ronald C. Petersen, Keith A. Josephs, Dennis W. Dickson, Michael E. Ward, Lijin Dong, Mercedes Prudencio, Casey N. Cook, Leonard Petrucelli
Publikováno v:
Frontiers in Cell and Developmental Biology, Vol 11 (2023)
Treatments for neurodegenerative disease, including Frontotemporal dementia (FTD) and Amyotrophic lateral sclerosis (ALS), remain rather limited, underscoring the need for greater mechanistic insight and disease-relevant models. Our ability to develo
Externí odkaz:
https://doaj.org/article/f5770006374045b4827be986a2b16eba