Identifying human pre-mRNA cleavage and polyadenylation factors by genome-wide CRISPR screens using a dual fluorescence readthrough reporter.

Autor: Ni Z; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, ON M5S 3E1, Canada., Ahmed N; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, ON M5S 3E1, Canada.; Department of Molecular Genetics, University of Toronto, 1 King's College Circle, Toronto, ON M5A 1A8, Canada., Nabeel-Shah S; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, ON M5S 3E1, Canada.; Department of Molecular Genetics, University of Toronto, 1 King's College Circle, Toronto, ON M5A 1A8, Canada., Guo X; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, ON M5S 3E1, Canada., Pu S; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, ON M5S 3E1, Canada., Song J; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, ON M5S 3E1, Canada., Marcon E; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, ON M5S 3E1, Canada., Burke GL; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, ON M5S 3E1, Canada.; Department of Molecular Genetics, University of Toronto, 1 King's College Circle, Toronto, ON M5A 1A8, Canada., Tong AHY; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, ON M5S 3E1, Canada.; Department of Molecular Genetics, University of Toronto, 1 King's College Circle, Toronto, ON M5A 1A8, Canada.; Program in Genetics and Genome Biology, The Hospital for Sick Children, Toronto, ON Canada., Chan K; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, ON M5S 3E1, Canada.; Department of Molecular Genetics, University of Toronto, 1 King's College Circle, Toronto, ON M5A 1A8, Canada.; Program in Genetics and Genome Biology, The Hospital for Sick Children, Toronto, ON Canada., Ha KCH; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, ON M5S 3E1, Canada.; Department of Molecular Genetics, University of Toronto, 1 King's College Circle, Toronto, ON M5A 1A8, Canada., Blencowe BJ; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, ON M5S 3E1, Canada.; Department of Molecular Genetics, University of Toronto, 1 King's College Circle, Toronto, ON M5A 1A8, Canada., Moffat J; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, ON M5S 3E1, Canada.; Department of Molecular Genetics, University of Toronto, 1 King's College Circle, Toronto, ON M5A 1A8, Canada.; Program in Genetics and Genome Biology, The Hospital for Sick Children, Toronto, ON Canada.; Institute for Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON Canada., Greenblatt JF; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, ON M5S 3E1, Canada.; Department of Molecular Genetics, University of Toronto, 1 King's College Circle, Toronto, ON M5A 1A8, Canada.

Publikováno v: Nucleic acids research [Nucleic Acids Res] 2024 May 08; Vol. 52 (8), pp. 4483-4501.

Synergistic cooperation between the β-catenin and SF1 regulates progesterone synthesis in laying hen ovarian granulosa cells.

Autor: Ma X; Department of Animal Genetics and Breeding, College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, China.; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an, Shandong, China.; Key Laboratory of Efficient Utilization of Non-grain Feed Resources (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Tai'an, Shandong, China., Han X; Department of Animal Genetics and Breeding, College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, China.; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an, Shandong, China.; Key Laboratory of Efficient Utilization of Non-grain Feed Resources (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Tai'an, Shandong, China., Zhang Q; Department of Animal Genetics and Breeding, College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, China.; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an, Shandong, China.; Key Laboratory of Efficient Utilization of Non-grain Feed Resources (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Tai'an, Shandong, China., Wang W; Department of Animal Genetics and Breeding, College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, China.; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an, Shandong, China.; Key Laboratory of Efficient Utilization of Non-grain Feed Resources (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Tai'an, Shandong, China., Tang H; Department of Animal Genetics and Breeding, College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, China.; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an, Shandong, China.; Key Laboratory of Efficient Utilization of Non-grain Feed Resources (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Tai'an, Shandong, China.

Publikováno v: Animal biotechnology [Anim Biotechnol] 2024 Nov; Vol. 35 (1), pp. 2351975. Date of Electronic Publication: 2024 May 14.

HIV-1 usurps mixed-charge domain-dependent CPSF6 phase separation for higher-order capsid binding, nuclear entry and viral DNA integration.

Autor: Jang S; Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Boston, MA 02215, USA.; Department of Medicine, Harvard Medical School, Boston, MA 02115, USA., Bedwell GJ; Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Boston, MA 02215, USA.; Department of Medicine, Harvard Medical School, Boston, MA 02115, USA., Singh SP; Institute of Molecular Biophysics, Department of Biological Sciences, Florida State University, Tallahassee, FL 32304, USA., Yu HJ; Model Development Section, Cancer Innovation Laboratory, National Cancer Institute, Frederick, MD 21702, USA., Arnarson B; Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Boston, MA 02215, USA., Singh PK; Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Boston, MA 02215, USA.; Department of Medicine, Harvard Medical School, Boston, MA 02115, USA., Radhakrishnan R; Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Boston, MA 02215, USA., Douglas AW; Institute of Molecular Biophysics, Department of Biological Sciences, Florida State University, Tallahassee, FL 32304, USA., Ingram ZM; Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219, USA., Freniere C; Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511, USA., Akkermans O; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Sarafianos SG; Center for ViroScience and Cure, Laboratory of Biochemical Pharmacology, Department of Pediatrics, School of Medicine, Emory University, Atlanta, GA 30322, USA., Ambrose Z; Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219, USA., Xiong Y; Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511, USA., Anekal PV; MicRoN Core, Harvard Medical School, Boston, MA 02215, USA., Montero Llopis P; MicRoN Core, Harvard Medical School, Boston, MA 02215, USA., KewalRamani VN; Model Development Section, Cancer Innovation Laboratory, National Cancer Institute, Frederick, MD 21702, USA., Francis AC; Institute of Molecular Biophysics, Department of Biological Sciences, Florida State University, Tallahassee, FL 32304, USA., Engelman AN; Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Boston, MA 02215, USA.; Department of Medicine, Harvard Medical School, Boston, MA 02115, USA.

Publikováno v: Nucleic acids research [Nucleic Acids Res] 2024 Oct 14; Vol. 52 (18), pp. 11060-11082.

Placenta-specific CYP11A1 overexpression lead to autism-like symptom in offspring with altered steroid hormone biosynthesis in the placenta-brain axis and rescued by vitamin D intervention.

Autor: Yin H; Joint Laboratory of Reproductive Medicine (SCU-CUHK), Key Laboratory of Birth Defects and Related Diseases of Women and Children of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu 610041, China; Chengdu Third People's Hospital, Chengdu 610041, China., Zhang J; Joint Laboratory of Reproductive Medicine (SCU-CUHK), Key Laboratory of Birth Defects and Related Diseases of Women and Children of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu 610041, China; Chengdu Third People's Hospital, Chengdu 610041, China., Chen Y; Joint Laboratory of Reproductive Medicine (SCU-CUHK), Key Laboratory of Birth Defects and Related Diseases of Women and Children of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu 610041, China., Guo J; Joint Laboratory of Reproductive Medicine (SCU-CUHK), Key Laboratory of Birth Defects and Related Diseases of Women and Children of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu 610041, China., Li Q; Joint Laboratory of Reproductive Medicine (SCU-CUHK), Key Laboratory of Birth Defects and Related Diseases of Women and Children of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu 610041, China., Dinnyes A; BioTalentum Ltd., Gödöllő, Hungary; Department of Physiology and Animal Health, Institute of Physiology and Animal Nutrition, Hungarian University of Agriculture and Life Sciences, Gödöllő, Hungary., Sun Q; Key Laboratory of Bio-Resource and Eco-Environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China., Liu X; Joint Laboratory of Reproductive Medicine (SCU-CUHK), Key Laboratory of Birth Defects and Related Diseases of Women and Children of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu 610041, China., He G; Joint Laboratory of Reproductive Medicine (SCU-CUHK), Key Laboratory of Birth Defects and Related Diseases of Women and Children of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu 610041, China., Zhu B; School of Life Science and Technology, Center for Information in Medicine, University of Electronic Science and Technology of China, China., Liu Y; Chengdu Third People's Hospital, Chengdu 610041, China., Xu P; School of Life Science and Technology, Center for Information in Medicine, University of Electronic Science and Technology of China, China. Electronic address: xupeng@uestc.edu.cn., Xu W; Joint Laboratory of Reproductive Medicine (SCU-CUHK), Key Laboratory of Birth Defects and Related Diseases of Women and Children of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu 610041, China. Electronic address: xuwenming@scu.edu.cn., Xie J; Chengdu Third People's Hospital, Chengdu 610041, China. Electronic address: xiejiang@swjtu.edu.cn.

Publikováno v: Brain, behavior, and immunity [Brain Behav Immun] 2024 Oct; Vol. 121, pp. 13-25. Date of Electronic Publication: 2024 Jul 16.

Structural insights into the diversity and DNA cleavage mechanism of Fanzor.

Autor: Xu P; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Cambridge, MA 02139, USA., Saito M; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Cambridge, MA 02139, USA., Faure G; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Cambridge, MA 02139, USA., Maguire S; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Cambridge, MA 02139, USA., Chau-Duy-Tam Vo S; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Cambridge, MA 02139, USA., Wilkinson ME; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Cambridge, MA 02139, USA., Kuang H; Cryo-Electron Microscopy Core, NYU Grossman School of Medicine, New York, NY 10016, USA., Wang B; Cryo-Electron Microscopy Core, NYU Grossman School of Medicine, New York, NY 10016, USA., Rice WJ; Cryo-Electron Microscopy Core, NYU Grossman School of Medicine, New York, NY 10016, USA; Department of Cell Biology, NYU Grossman School of Medicine, New York, NY 10016, USA., Macrae RK; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Cambridge, MA 02139, USA., Zhang F; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Cambridge, MA 02139, USA. Electronic address: zhang@broadinstitute.org.

Publikováno v: Cell [Cell] 2024 Sep 19; Vol. 187 (19), pp. 5238-5252.e20. Date of Electronic Publication: 2024 Aug 28.