Zobrazeno 1 - 10
of 323
pro vyhledávání: '"cell separation/fractionation"'
Publikováno v:
STAR Protocols, Vol 5, Iss 4, Pp 103484- (2024)
Summary: Currently, the only approach for obtaining alveolar epithelial type I (AT1) cells is through the differentiation of cultured AT2 cells, which has several limitations. Here, we present a protocol to prepare AT1 cells directly from mouse lungs
Externí odkaz:
https://doaj.org/article/3b5fbae1233c4a79a6283fcf85f7867e
Publikováno v:
STAR Protocols, Vol 5, Iss 4, Pp 103451- (2024)
Summary: Most common techniques for isolating eosinophils utilize CD16-negative selection, neglecting the CD16-positive fraction of eosinophils. Here, we present a protocol for isolating human CD16+ and CD16− eosinophils based on their autofluoresc
Externí odkaz:
https://doaj.org/article/194efd33d2a14ec99f3869334af165f9
Publikováno v:
STAR Protocols, Vol 5, Iss 4, Pp 103424- (2024)
Summary: We outline a protocol to visualize all mouse lower hindlimb skeletal muscles simultaneously. We describe procedures for orientating the whole lower hindlimb in gum tragacanth prior to freezing, simplifying the proceeding experimental steps,
Externí odkaz:
https://doaj.org/article/985dfdcf80014e4e8e3a92f2fc0d6596
Autor:
Yaling Huang, Yisha Wu, Shikai Han, Qiaoling Wang, Guomingxiu Cong, Zhongzhen Liu, Shuyan Guan, Xiaojuan Huang, Ying Liu, Jianhua Yin, Jinmei Xue, Chuanyu Liu
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103298- (2024)
Summary: Examining nasal mucosa samples is crucial for nasal cavity disease research and diagnosis. Simultaneously obtaining high-quality data for single-cell transcriptomics (single-cell RNA sequencing [scRNA-seq]) and epigenomics (single-cell assay
Externí odkaz:
https://doaj.org/article/f04802d27fb74c6caf81f746d18e65e1
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103253- (2024)
Summary: Dynamic communication between intracellular organelles often takes place at specialized membrane contact sites that form between their membranes. Here we detail a procedure for the purification of endoplasmic reticulum-plasma membrane (ER-PM
Externí odkaz:
https://doaj.org/article/d063f8c374a34bb3a644ddba48c24647
Autor:
Ernesto Gargiulo, Pablo Elias Morande, Maxmilan Jeyakumar, Lucie Rospape, Jérôme Paggetti, Etienne Moussay
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103244- (2024)
Summary: Here, we present a protocol for the direct isolation of small extracellular vesicles (sEVs) from the spleen of preclinical murine models of leukemia using ultracentrifugation. We describe steps for tissue collection, sample preparation, ultr
Externí odkaz:
https://doaj.org/article/c477576d1f794bb5ae2e4d4a651d8847
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103208- (2024)
Summary: Protein kinase C-δ (PKC-δ) is a key enzyme controlling growth, differentiation, and apoptosis in various cells, including immune cells. Here, we present a protocol to determine PKC-δ activation in response to increased membrane-bound diac
Externí odkaz:
https://doaj.org/article/b627674bb4454d0da0c2ed8adaab8935
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103174- (2024)
Summary: Isolating high-quality different cell types is a powerful approach for understanding cellular compositions and features in the heart, but it is challenging. The available protocols typically focus on isolating one or two cell types. Here, we
Externí odkaz:
https://doaj.org/article/517d45c4278447b3919605137706581e
Autor:
Clément Maghe, Julie Gavard
Publikováno v:
STAR Protocols, Vol 5, Iss 2, Pp 103121- (2024)
Summary: Lysosomes are critical for the sustenance of glioblastoma stem-like cells (GSCs) properties. We present a protocol to enrich and purify lysosomes from patient-derived GSCs in culture. We describe the steps required to stably express a tagged
Externí odkaz:
https://doaj.org/article/81ef1a304d984fe8a00b6c29e0d1bdbb
Autor:
Charlotte A. Townsend, Andrey A. Petropavlovskiy, Jordan A. Kogut, Alysha M. Church, Shaun S. Sanders
Publikováno v:
STAR Protocols, Vol 5, Iss 2, Pp 103068- (2024)
Summary: S-acylation, commonly palmitoylation, is the addition of fatty acids to cysteines to regulate protein localization and function. S-acylation detection has been hampered by limited sensitivity and selectivity in low-protein, costly samples li
Externí odkaz:
https://doaj.org/article/3a308ca501bd42ad915b92d2a309317b