Optimisation of metabarcoding for monitoring marine macrobenthos: primer choice and morphological traits determine species detection in bulkDNA and eDNA from the ethanol preservative

Autor: Annelies De Backer, Annelies Haegeman, Sara Maes, Sofie Derycke, Kris Hostens, Hans Hillewaert, Bart Ampe, Jan Wittoeck, Laure Van den Bulcke, Joran Vanhollebeke

DNA metabarcoding is a promising method to increase cost and time efficiency of marine monitoring, providing that the impact of methodological choices on the reliability and reproducibility of results are well understood. Here, we investigated the im

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::fd185711beb0b3d05abcf6624fb6f850
https://doi.org/10.22541/au.159665093.39993653

High‐throughput sequencing on preservative ethanol is effective at jointly examining infraspecific and taxonomic diversity, although bioinformatics pipelines do not perform equally

Autor: Couton, Marjorie, Baud, Aurélien, Daguin-Thiébaut, Claire, Corre, Erwan, Comtet, Thierry, Viard, Frédérique

Publikováno v: Ecology and Evolution
Ecology and Evolution, Wiley Open Access, 2021, 11 (10), pp.5533-5546. ⟨10.1002/ece3.7453⟩
Ecology and Evolution, Vol 11, Iss 10, Pp 5533-5546 (2021)
Ecology and Evolution, 2021, 11 (10), pp.5533-5546. ⟨10.1002/ece3.7453⟩

High‐throughput sequencing of amplicons (HTSA) has been proposed as an effective approach to evaluate taxonomic and genetic diversity at the same time. However, there are still uncertainties as to how the results produced by different bioinformatic

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::457cf2f682d804755039a01fb5e9f0fd
http://europepmc.org/articles/PMC8131761

High clonal diversity and spatial genetic admixture in early prostate cancer and surrounding normal tissue.

Autor: Ning Zhang, Harbers, Luuk, Simonetti, Michele, Diekmann, Constantin, Verron, Quentin, Berrino, Enrico, Bellomo, Sara E., Longo, Gabriel M. C., Ratz, Michael, Schultz, Niklas, Tarish, Firas, Peng Su, Bo Han, Wanzhong Wang, Onorato, Sofia, Grassini, Dora, Ballarino, Roberto, Giordano, Silvia, Qifeng Yang, Sapino, Anna

Publikováno v: Nature Communications; 4/24/2024, Vol. 15 Issue 1, p1-17, 17p, 3 Graphs

DNA metabarcoding on repeat: Sequencing data of marine macrobenthos are reproducible and robust across labs and protocols

Autor: Laure Van den Bulcke, Annelies De Backer, Jan Wittoeck, Kevin Beentjes, Sara Maes, Magdalini Christodoulou, Pedro Martinez Arbizu, Rumakanta Sapkota, Berry Van der Hoorn, Anne Winding, Kris Hostens, Sofie Derycke

Publikováno v: Ecological Indicators, Vol 150, Iss , Pp 110207- (2023)

DNA metabarcoding can be used in marine environmental monitoring if results are reproducible between labs and robust against modifications to the lab protocol. In this interlaboratory study, we conducted a ring test where subsamples of blended macrob

Externí odkaz: https://doaj.org/article/b69da525fc334abb87e31d1218eecc2f

Simple Is the Best: An Alternative Method for the Analysis of Free-Living Nematode Assemblage Structure.

Autor: Semprucci, Federica, Grassi, Eleonora, Balsamo, Maria

Publikováno v: Water (20734441); Apr2022, Vol. 14 Issue 7, p1114, 16p