Zobrazeno 1 - 10
of 89
pro vyhledávání: '"and Arthur G. Szabo"'
Autor:
Grant E. Blouse, Linda A. Luck, Arthur G. Szabo, Joseph D. Shore, Michel J. Perron, Glenn L. Abbott
Publikováno v:
Biochemistry. 43:1507-1519
Plasminogen activator inhibitor-1 (PAI-1) is a 43 kDa protein involved in the regulation of fibrinolysis. PAI-1 is the principal inhibitor of tissue-type plasminogen activator (t-PA), trapping the proteinase as an acyl-enzyme covalent complex (approx
Autor:
Susan M. Twine, Arthur G. Szabo, Patrik Callis, Michael T. Cash, Lise Murphy, Robert S. Phillips
Publikováno v:
The Journal of Physical Chemistry B. 107:637-645
The biosynthetic incorporation of tryptophan analogues into proteins using plasmid expression systems has been shown to offer advantages in fluorescence spectroscopic studies of the molecular details of the interacting segments of protein−protein c
Autor:
Kevin J. Willis, John D. Brennan, Arthur G. Szabo, Bakirathan Rajendran, Christopher W.V. Hogue
Publikováno v:
Analytical Biochemistry. 252:260-270
The reaction of D,L-7-azatryptophan (D,L-7AW) with tryptophanyl-tRNA synthetase (TrpRS), adenosine triphosphate (ATP), and Mg2+ in the presence of inorganic pyrophosphatase results in the formation of a highly fluorescent l-7AW-adenylate complex. Det
Autor:
Hong Xue, Charles W. Carter, Arthur G. Szabo, Jeffery T. Wong, Christopher W. V. Hogue, Sylvie Doublié
Publikováno v:
Journal of Molecular Biology. 260:446-466
A semi-conserved tryptophan residue of Bacillus subtilis tryptophanyl-tRNA synthetase (TrpRS) was previously asserted to be an essential residue and directly involved in tRNATrp binding and recognition. The crystal structure of the Bacillus stearothe
Publikováno v:
Biochemistry. 35:531-537
The ligand-induced conformational change of an active lysozyme derivative, Kyn62-lysozyme, in which Trp62 of hen egg-white lysozyme (EC 3.2.1.17) was selectively modified to kynurenine, was investigated by steady-state and time-resolved fluorescence
Publikováno v:
Biophysical Journal. 104(2)
atToc33 and psToc34 are GTPases-, that act as receptors for pre-protein import in the chloroplast outer envelope. Various lines of evidence suggest that the GTPase domain of atToc33 (atToc33G) dimerizes, including native PAGE studies (Weibel et al.,
Publikováno v:
Journal of the American Chemical Society. 117:2321-2326
Autor:
Andromeda J. Bruckman, Ian D. Clark, Christopher W. V. Hogue, John P. MacManus, Arthur G. Szabo
Publikováno v:
Journal of Fluorescence. 4:235-241
The Ca(2+)-binding protein oncomodulin was altered by cassette mutagenesis of the CD site (CDOM33) with a sequence that was derived by a consensus method using over 250 known Ca(2+)-binding loop sequences. This mutant was studied using time-resolved
Publikováno v:
Analytical Biochemistry. 216:439-443
Methods were developed for using the luminescent lanthanides Tb3+ and Eu3+ for the specific staining of calcium-binding proteins, as well as the nonspecific staining of proteins, on polyacrylamide gels. These methods involve equilibration of the gel
Autor:
Alberto Mazzini, Lorella Franzoni, Paolo Cavatorta, Alberto Spisni, Arthur G. Szabo, Roberto Favilla
Publikováno v:
Journal of Fluorescence. 3:211-214
The conformation of the nonapeptide hormone litorin has been studied in buffer and in the presence of lipids, using static and dynamic fluorescence. The results obtained show that, in buffer, the hormone probably exists in a collection of flexible co