Zobrazeno 1 - 10
of 210
pro vyhledávání: '"amplification efficiency"'
Publikováno v:
IEEE Photonics Journal, Vol 15, Iss 1, Pp 1-10 (2023)
We present an experimental system study of the influence of cryogenic temperatures on the performance of a commercial Semiconductor Optical Amplifier (SOA) over the range 240 K – 70 K. Significant performance improvements were observed when the SOA
Externí odkaz:
https://doaj.org/article/642dc4b376aa4e58bf51fc71d154848e
Autor:
Philip J. Schmidt, Nicole Acosta, Alex H. S. Chik, Patrick M. D’Aoust, Robert Delatolla, Hadi A. Dhiyebi, Melissa B. Glier, Casey R. J. Hubert, Jennifer Kopetzky, Chand S. Mangat, Xiao-Li Pang, Shelley W. Peterson, Natalie Prystajecky, Yuanyuan Qiu, Mark R. Servos, Monica B. Emelko
Publikováno v:
Frontiers in Microbiology, Vol 14 (2023)
The real-time polymerase chain reaction (PCR), commonly known as quantitative PCR (qPCR), is increasingly common in environmental microbiology applications. During the COVID-19 pandemic, qPCR combined with reverse transcription (RT-qPCR) has been use
Externí odkaz:
https://doaj.org/article/90aaba847522444d9f47346ac20f211f
Autor:
GAURAV DHAWAN, VIJAY PRAKASH, ARUNA KUMAR, PANKAJ KUMAR, RATHOUR RAJEEV, ASHOK KUMAR SINGH, GOPALA KRISHNAN S
Publikováno v:
The Indian Journal of Agricultural Sciences, Vol 91, Iss 3 (2022)
PCR amplification and detection of GC rich sequences in DNA is a challenge due to formation of secondary structures which resist denaturation, thereby stalling Taq DNA polymerases as well as affecting primer annealing. Presently, high fidelity Taq DN
Externí odkaz:
https://doaj.org/article/d360ad527ed7467e8e6812e28c0daf02
Publikováno v:
BMC Biotechnology, Vol 21, Iss 1, Pp 1-10 (2021)
Abstract Background Although quantitative real-time PCR (qRT-PCR) is a common and sensitive method for miRNAs analysis, it is necessary to optimize conditions and minimize qRT-PCR inhibitors to achieve reliable results. The aim of this study was to m
Externí odkaz:
https://doaj.org/article/773d7f6926b44967bf7c0718b5993587
Autor:
Joel Tellinghuisen
Publikováno v:
BMC Bioinformatics, Vol 21, Iss 1, Pp 1-11 (2020)
Abstract Background A recently proposed method for estimating qPCR amplification efficiency E analyzes fluorescence intensity ratios from pairs of points deemed to lie in the exponential growth region on the amplification curves for all reactions in
Externí odkaz:
https://doaj.org/article/e8e0b8cab0b9406c81f883406aa839b9
Publikováno v:
Frontiers in Genetics, Vol 12 (2021)
Background: Precise determination of amplification efficiency is critical for reliable conversion of within-sample changes in fluorescence occurring on a logarithmic scale to between-sample differences in DNA content occurring on a linear scale. This
Externí odkaz:
https://doaj.org/article/d27ddcd56dad412e82de45049fbe83c1
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Autor:
Brown, Katherine L., Ceci, Alessandro, Roby, Clinton, Briggs, Russell B., Ziolo, D., Korba, R., Mejia, R., Kelly, S. T., Toney, D., Friedlander, Michael J., Finkielstein, Carla V.
Mutations in the SARS-CoV-2 genome may negatively impact a diagnostic test, have no effect, or turn into an opportunity for rapid molecular screening of variants. Using an in-house Emergency Use Authorized RT-qPCR-based COVID-19 diagnostic assay, we
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=od______2485::6624ddf36bb5f14ff0af659b3d2693be
https://hdl.handle.net/10919/114137
https://hdl.handle.net/10919/114137
Akademický článek
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