Zobrazeno 1 - 10
of 201
pro vyhledávání: '"amplification bias"'
Autor:
Burcu Gurun, Wesley Horton, Dhaarini Murugan, Biqing Zhu, Patrick Leyshock, Sushil Kumar, Katelyn T. Byrne, Robert H. Vonderheide, Adam A. Margolin, Motomi Mori, Paul T. Spellman, Lisa M. Coussens, Terence P. Speed
Publikováno v:
BMC Genomics, Vol 24, Iss 1, Pp 1-13 (2023)
Abstract T cell receptor repertoires can be profiled using next generation sequencing (NGS) to measure and monitor adaptive dynamical changes in response to disease and other perturbations. Genomic DNA-based bulk sequencing is cost-effective but nece
Externí odkaz:
https://doaj.org/article/f072a81ae5e44edd9681ef3c1accf20e
Autor:
Jin Sung Jang, Eileen Holicky, Julie Lau, Samantha McDonough, Mark Mutawe, Matthew J. Koster, Kenneth J. Warrington, Julie M. Cuninngham
Publikováno v:
BMC Genomics, Vol 22, Iss 1, Pp 1-9 (2021)
Abstract Background Archival formalin-fixed, paraffin-embedded (FFPE) tissue samples with clinical and histological data are a singularly valuable resource for developing new molecular biomarkers. However, transcriptome analysis remains challenging w
Externí odkaz:
https://doaj.org/article/d18cfbe9b1934138adbf514a838ede3a
Publikováno v:
PeerJ, Vol 10, p e13888 (2022)
High-throughput sequencing of amplicon libraries is the most widespread and one of the most effective ways to study the taxonomic structure of microbial communities, even despite growing accessibility of whole metagenome sequencing. Due to the target
Externí odkaz:
https://doaj.org/article/96121f3fdc7b47a3a36820e7aa1f42b4
Publikováno v:
New Phytologist, 2018 Feb 01. 217(3), 1370-1385.
Externí odkaz:
https://www.jstor.org/stable/90018561
Akademický článek
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Publikováno v:
Frontiers in Genetics, Vol 11 (2020)
Identification of de novo copy number variations (CNVs) across the genome in single cells requires single-cell whole-genome amplification (WGA) and sequencing. Although many experimental protocols of amplification methods have been developed, all suf
Externí odkaz:
https://doaj.org/article/dbe66f68a0094bf3992a6957189895e5
Autor:
Simon Poirier, Olivier Rué, Gwendoline Coeuret, Marie-Christine Champomier-Vergès, Valentin Loux, Stéphane Chaillou
Publikováno v:
BMC Research Notes, Vol 11, Iss 1, Pp 1-5 (2018)
Abstract Objectives Sequencing of 16S rDNA V3–V4 region is widely applied for food community profiling. However, two different universal forward primers (named here MUYZER-primer1 and KLINDWORTH-primer2) targeting an identical conservative sequence
Externí odkaz:
https://doaj.org/article/7d5b6345eb2c4f0ca0b56fd930efd0c2
Publikováno v:
Genes; Volume 14; Issue 4; Pages: 935
The species delimitation of the marine bivalve species complex Aequiyoldia eightsii in South America and Antarctica is complicated by mitochondrial heteroplasmy and amplification bias in molecular barcoding. In this study, we compare different data s
Autor:
Bastiaan Star, Marianne HS Hansen, Morten Skage, Ian R. Bradbury, Jane A. Godiksen, Olav S. Kjesbu, Sissel Jentoft
Publikováno v:
Science and Technology of Archaeological Research, Vol 2, Iss 1, Pp 36-45 (2016)
Repetitive microsatellite DNA forms a universal component of eukaryote genomes and specific biochemical properties of such repeat regions may influence the outcome of laboratory protocols. The Atlantic cod (Gadus morhua) genome contains an order of m
Externí odkaz:
https://doaj.org/article/f12381567ed8472e84da941fc96a67bb
Akademický článek
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