A Novel Dimeric Exoglucanase (GH5_38): Biochemical and Structural Characterisation towards its Application in Alkyl Cellobioside Synthesis

Autor: Mpho S. Mafa, Heinrich W. Dirr, Samkelo Malgas, Rui W. M. Krause, Konanani Rashamuse, Brett I. Pletschke

Publikováno v: Molecules, Vol 25, Iss 3, p 746 (2020)

An exoglucanase (Exg-D) from the glycoside hydrolase family 5 subfamily 38 (GH5_38) was heterologously expressed and structurally and biochemically characterised at a molecular level for its application in alkyl glycoside synthesis. The purified Exg-

Externí odkaz: https://doaj.org/article/8ebc770c33f847d9a78644adf3206b35

A single point mutation alters the transglycosylation/hydrolysis partition, significantly enhancing the synthetic capability of an endo-glycoceramidase

Autor: Julien Durand, Michael J. O’Donohue, Antoni Planas, Laurie Watterlot, Sophie Bozonnet, Xevi Biarnés, Cyrielle Bonzom, Vinciane Borsenberger, Régis Fauré

Publikováno v: ACS Catalysis
ACS Catalysis, 2016, 6 (12), pp.8264-8275. ⟨10.1021/acscatal.6b02159⟩
ACS Catalysis, American Chemical Society, 2016, 6 (12), pp.8264-8275. ⟨10.1021/acscatal.6b02159⟩

The mutation of D311 to tyrosine in endo-glycoceramidase II from Rhodococcus sp. and the use of a poorly recognized substrate, 2-chloro-4-nitrophenyl beta-cellobioside, have provided appropriate conditions for the efficient synthesis of alkyl beta-ce

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6997333999dc122d59973435568777f0
https://hal.science/hal-01606355