Zobrazeno 1 - 10
of 8 238
pro vyhledávání: '"agarose gel"'
Publikováno v:
Journal of Microbiology & Biology Education, Vol 25, Iss 3 (2024)
ABSTRACT DNA detection by agarose gel electrophoresis (AGE) is commonly used in molecular biology. AGE is a separation method that provides opportunities for students to learn about the topology and size of DNA molecules. Recently, several fluorescen
Externí odkaz:
https://doaj.org/article/ed430ddcfa67445196bd26f720523ca4
Autor:
Jorge Cebrián, María-Luisa Martínez-Robles, Victor Martínez, Pablo Hernández, Dora B. Krimer, Jorge B. Schvartzman, María-José Fernández-Nestosa
Publikováno v:
DNA, Vol 4, Iss 2, Pp 170-179 (2024)
DNA knots are deleterious for living cells if not removed. Several theoretical and simulation approaches address the question of how topoisomerases select the intermolecular passages that preferentially lead to unknotting rather than to the knotting
Externí odkaz:
https://doaj.org/article/7ac973adfbe34014a1daff3d903d6857
Autor:
James P. Chambers, Miranda Aldis, Julie A. Thomas, Cara B. Gonzales, Richard Allen White, Philip Serwer
Publikováno v:
Biophysica, Vol 4, Iss 2, Pp 195-206 (2024)
Past anti-bacterial use of bacteriophages (phage therapy) is already well reviewed as a potential therapeutic response to the emergence of multidrug-resistant, pathogenic bacteria. Phage therapy has been limited by the following. (1) The success rate
Externí odkaz:
https://doaj.org/article/673047e8805e4bbf8c9310378e4e47e5
Autor:
Vicky Wilkinson, Shane A. Richards, Scott Carver, Christina Næsborg-Nielsen, Carolyn Cray, Gabriele Rossi
Publikováno v:
Frontiers in Veterinary Science, Vol 11 (2024)
Sarcoptic mange, caused by epidermal infection with Sarcoptes scabiei, negatively impacts the health, welfare, and local abundance of bare-nosed wombats (Vombatus ursinus) in Australia. Improved understanding of the host immune response to disease an
Externí odkaz:
https://doaj.org/article/a2f6e84fc8a3444488f7faa1588d3afc
Autor:
Shirou Tsuchida, Takaaki Hirayama, Hayato Nunose, Hinako Suzuki, Ryo Hakota, Tsugumi Shindo, Koji Nakagawa
Publikováno v:
Heliyon, Vol 10, Iss 9, Pp e30465- (2024)
A new UGT1A1*28 detection method combining PCR and high-resolution agarose gel electrophoresis was developed. The viability of this method was demonstrated on 15 healthy adult volunteers. Subjects included 13 wild type homozygotes (86.7 %), 2 heteroz
Externí odkaz:
https://doaj.org/article/0288877df77d404c850deb7afba6ec03
Publikováno v:
In Heliyon 30 January 2024 10(2)
Publikováno v:
Heliyon, Vol 10, Iss 2, Pp e24451- (2024)
Two simple and inexpensive in-house qualitative human immunodeficiency virus type 1 nucleotide amplification tests (HIV-1 NATs) were established as adjunct confirmatory HIV test for HIV antigen (Ag)-positive specimens identified from HIV screening te
Externí odkaz:
https://doaj.org/article/e906068b21f34982ba19a994e6dc0778
Autor:
L.A. Lefol, P. Bawuah, J.A. Zeitler, J. Verin, F. Danede, J.F. Willart, F. Siepmann, J. Siepmann
Publikováno v:
International Journal of Pharmaceutics: X, Vol 6, Iss , Pp 100220- (2023)
This study aimed to evaluate and better understand the potential impact that a layer of surrounding hydrogel (mimicking living tissue) can have on the drug release from PLGA microparticles. Ibuprofen-loaded microparticles were prepared with an emulsi
Externí odkaz:
https://doaj.org/article/8eab003d5b2b4d1682a8b314fd2498b0
Autor:
Ryosuke Ishii
Publikováno v:
Genetics & Applications, Vol 7, Iss 2 (2023)
Agarose gel electrophoresis is a routinely employed experimental technique for visualizing nucleic acids, typically combined with ethidium bromide or some other molecular dye staining and a UV transilluminator. However, the utilization of a UV transi
Externí odkaz:
https://doaj.org/article/917470fc10d744d5af2073e068274ce4
Publikováno v:
DNA, Vol 2, Iss 4, Pp 302-313 (2022)
Understanding the self-assembly and hybridization properties of DNA oligonucleotides in confined spaces can help to improve their applications in biotechnology and nanotechnology. This study investigates the effects of spatial confinement in the pore
Externí odkaz:
https://doaj.org/article/2b061a84aca143b1835fce2a568f13ea