A generic HTS assay for kinase screening: Validation for the isolation of an engineered malate kinase

Autor: Isabelle André, Nelly Martineau, Audrey Baylac, Clément Auriol, Magali Remaud-Simeon, Jean-Marie François, Christopher M. Topham, Romain Irague, Thomas Walther

Publikováno v: PLoS ONE
PLoS ONE, Public Library of Science, 2018, 13 (2), 15 p. ⟨10.1371/journal.pone.0193036⟩
PLoS ONE, 2018, 13 (2), 15 p. ⟨10.1371/journal.pone.0193036⟩
PLoS ONE, Vol 13, Iss 2, p e0193036 (2018)
Plos One 2 (13), 15 p.. (2018)

An end-point ADP/NAD(+) acid/alkali assay procedure, directly applicable to library screening of any type of ATP-utilising/ADP producing enzyme activity, was implemented. Typically, ADP production is coupled to NAD(+) co-enzyme formation by the conve

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https://hal.archives-ouvertes.fr/hal-01886455/file/journal.pone.0193036.pdf