CRISPR/Cas9 and active genetics-based trans-species replacement of the endogenous Drosophila kni-L2 CRM reveals unexpected complexity

Autor: Xiang-Ru Shannon Xu, Valentino Matteo Gantz, Natalia Siomava, Ethan Bier

Publikováno v: eLife, Vol 6 (2017)

The knirps (kni) locus encodes transcription factors required for induction of the L2 wing vein in Drosophila. Here, we employ diverse CRISPR/Cas9 genome editing tools to generate a series of targeted lesions within the endogenous cis-regulatory modu

Externí odkaz: https://doaj.org/article/690b5a3ca24a43ab9a1dae3beb01ec97

Active Genetic Neutralizing Elements for Halting or Deleting Gene Drives

Autor: Xu, Xiang-Ru Shannon, Bulger, Emily A, Gantz, Valentino M, Klanseck, Carissa, Heimler, Stephanie R, Auradkar, Ankush, Bennett, Jared B, Miller, Lauren Ashley, Leahy, Sarah, Juste, Sara Sanz, Buchman, Anna, Akbari, Omar S, Marshall, John M, Bier, Ethan

Publikováno v: Molecular cell, vol 80, iss 2

CRISPR-Cas9-based gene drive systems possess the inherent capacity to spread progressively throughout target populations. Here we describe two self-copying (or active) guide RNA-only genetic elements, called e-CHACRs and ERACRs. These elements use Ca

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::118d0e501ba67e695748a8b780381b4c
https://escholarship.org/uc/item/6w66g5dm

Deficient LRRC8A-dependent volume-regulated anion channel activity is associated with male infertility in mice

Autor: Taiping Chen, John H. Richburg, Sharon Y.R. Dent, Raif S. Geha, Jean Louis Guénet, Caitlin J. Murphy, Carlos J. Perez, Janet Chou, Jean Jaubert, Tewfik Hamidi, Meichun Deng, Craig D. Platt, Qinghua Shi, Yue Lu, Héctor Gaitán-Peñas, Fernando Benavides, Kevin Lin, Jeesun Kim, Hui Lin Pan, Mark T. Bedford, Jianqiang Bao, Meng Hua Zhou, Huan Zhang, Yuying Huang, Raúl Estévez

Publikováno v: Dipòsit Digital de la UB
Universidad de Barcelona
JCI Insight
JCI Insight, American Society for Clinical Investigation, 2018, 3 (16), pp.e99767. ⟨10.1172/jci.insight.99767⟩
JCI Insight, 2018, 3 (16), pp.e99767. ⟨10.1172/jci.insight.99767⟩

International audience; Ion channel-controlled cell volume regulation is of fundamental significance to the physiological function of sperm. In addition to volume regulation, LRRC8A-dependent volume-regulated anion channel (VRAC) activity is involved

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fb59899d04360969f84ccb27e341fc46
http://hdl.handle.net/2445/172306

Caspofungin activity against clinical isolates of azole cross-resistant Candida glabrata overexpressing efflux pump genes

Autor: Barbara Fiori, Maurizio Sanguinetti, Brunella Posteraro, Marilena La Sorda, Dominique Sanglard, Teresa Spanu, Giovanni Fadda

Publikováno v: The Journal of Antimicrobial Chemotherapy, vol. 58, no. 2, pp. 458-461

OBJECTIVES: Several studies have documented the potent in vitro activity of caspofungin against Candida spp. This is of special concern for Candida glabrata infections that are often resistant to many azole antifungal agents and, consequently, diffic

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::059752ee96b1f76711d9de6df6df15a9
http://doc.rero.ch/record/304767/files/dkl237.pdf

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