Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Zuriah A. Meacham"'
A genome-scale CRISPR interference guide library enables comprehensive phenotypic profiling in yeast
Autor:
Nicholas J. McGlincy, Zuriah A. Meacham, Kendra K. Reynaud, Ryan Muller, Rachel Baum, Nicholas T. Ingolia
Publikováno v:
BMC Genomics, Vol 22, Iss 1, Pp 1-17 (2021)
Abstract Background CRISPR/Cas9-mediated transcriptional interference (CRISPRi) enables programmable gene knock-down, yielding loss-of-function phenotypes for nearly any gene. Effective, inducible CRISPRi has been demonstrated in budding yeast, and g
Externí odkaz:
https://doaj.org/article/aec3ad964ab64531893552e4f3667382
Autor:
Celeste Riepe, Elena Zelin, Phillip A. Frankino, Zuriah A. Meacham, Samantha G. Fernandez, Nicholas T. Ingolia, Jacob E. Corn
Publikováno v:
The FEBS Journal, 289 (11)
DNA damage activates a robust transcriptional stress response, but much less is known about how DNA damage impacts translation. The advent of genome editing with Cas9 has intensified interest in understanding cellular responses to DNA damage. Here, w
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fe61567d7d91d27c9cc2ab25771b6289
https://doi.org/10.1111/febs.16321
https://doi.org/10.1111/febs.16321
Publikováno v:
Bio Protoc
Genetic networks regulate nearly all biological processes, including cellular differentiation, homeostasis, and immune responses. Determining the precise role of each gene within a regulatory network can explain its overall, integrated function, and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::d6857fe44a379f9e2f57b0cd91633acd
https://pubmed.ncbi.nlm.nih.gov/35530514
https://pubmed.ncbi.nlm.nih.gov/35530514
A genome-scale CRISPR interference guide library enables comprehensive phenotypic profiling in yeast
Autor:
Kendra Swain, Nicholas T. Ingolia, Rachel Baum, Zuriah A. Meacham, Nicholas J. McGlincy, Ryan Muller
Publikováno v:
BMC Genomics
BMC genomics, vol 22, iss 1
BMC Genomics, Vol 22, Iss 1, Pp 1-17 (2021)
BMC genomics, vol 22, iss 1
BMC Genomics, Vol 22, Iss 1, Pp 1-17 (2021)
Background CRISPR/Cas9-mediated transcriptional interference (CRISPRi) enables programmable gene knock-down, yielding loss-of-function phenotypes for nearly any gene. Effective, inducible CRISPRi has been demonstrated in budding yeast, and genome-sca
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d378ee87a9989148df479ef84dcf7a4
http://europepmc.org/articles/PMC7986282
http://europepmc.org/articles/PMC7986282
Autor:
McGlincy, Nicholas J., Zuriah A. Meacham, Reynaud, Kendra K., Muller, Ryan, Baum, Rachel, Ingolia, Nicholas T.
Additional file 7: Figure S3. Receiver operating characteristic for logistic regression models of guide activity.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2fe77d69c5cdb2d0a33de004704c5aed
Autor:
McGlincy, Nicholas J., Zuriah A. Meacham, Reynaud, Kendra K., Muller, Ryan, Baum, Rachel, Ingolia, Nicholas T.
Additional file 8: Table S8. Table of custom oligonucleotide sequences used in this study.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2831823f35d9e6dd00496929ef92993a
Publikováno v:
Science (New York, N.Y.), vol 370, iss 6522
Science
Science
CiBER-seq dissects genetic networks Cells integrate environmental signals and internal states to dynamically control gene expression. Muller et al. developed a technique to dissect this cellular logic by linking targeted, genome-wide genetic perturba
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d5b894b46eb84680bfbdddb5f1c55222
https://doi.org/10.1101/2020.03.29.015057
https://doi.org/10.1101/2020.03.29.015057
Double Stranded DNA Breaks and Genome Editing Trigger Ribosome Remodeling and Translational Shutdown
Autor:
Elena Zelin, Nicholas T. Ingolia, Alexander Marson, Jr Liang, Jonathan T. Vu, Jacob E. Corn, C Riepe, Stacia K. Wyman, Phillip A. Frankino, David N. Nguyen, Zuriah A. Meacham
SummaryDNA damage activates a robust transcriptional stress response, but much less is known about how DNA impacts translation. The advent of genome editing via a Cas9-induced DNA double-strand break has intensified interest in understanding cellular
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3f549f80682a83164078ed3ed1630660
We present an accessible, robust continuous-culture turbidostat system that enables biologists to generate and phenotypically analyze highly complex libraries in yeast and bacteria. Our system has many applications in genomics and systems biology; he
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5334b44ac1302fc61ca40955a6fece91
Autor:
McGlincy, Nicholas J., Zuriah A. Meacham, Reynaud, Kendra K., Muller, Ryan, Baum, Rachel, Ingolia, Nicholas T.
Additional file 2: Figures S1 and S2. Detailed strategy for library construction and sequencing.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d79835fd6708c946d0f8248257355b8f