Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Zizhuo Xing"'
Autor:
Zizhuo Xing, Chao Huang, Xuankuo Xu, Weixin Jin, Michael Borys, Zheng Jian Li, Sanchayita Ghose, Yuanli Song
Publikováno v:
Biotechnology and bioengineeringREFERENCES. 117(11)
Significant amounts of soluble product aggregates were observed in the low-pH viral inactivation (VI) operation during an initial scale-up run for an immunoglobulin-G 4 (IgG4) monoclonal antibody (mAb IgG4-N1). Being earlier in development, a scale-d
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e0021c98c79e3377e2fa6c2afe83f730
https://pubmed.ncbi.nlm.nih.gov/32672835
https://pubmed.ncbi.nlm.nih.gov/32672835
Publikováno v:
Biotechnology and Bioengineering. 114:1184-1194
Control of carbon dioxide within the optimum range is important in mammalian bioprocesses at the manufacturing scale in order to ensure robust cell growth, high protein yields, and consistent quality attributes. The majority of bioprocess development
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fa89ccb129422eae4bedc90321bf2e0b
https://doi.org/10.1002/bit.26232
https://doi.org/10.1002/bit.26232
Autor:
Chao Huang, Zizhuo Xing, Sanchayita Ghose, Xuankuo Xu, Yuanli Song, Weixin Jin, Zheng Jian Li
Publikováno v:
MAbs
Significant amounts of soluble product aggregates were observed during low-pH viral inactivation (VI) scale-up for an IgG4 monoclonal antibody (mAb IgG4-N1), while small-scale experiments in the same condition showed negligible aggregation. Poor mixi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c5dbac5d310215118e43395e0d3f602a
https://europepmc.org/articles/PMC6816434/
https://europepmc.org/articles/PMC6816434/
Publikováno v:
Biotechnology and bioengineering. 114(6)
Control of carbon dioxide within the optimum range is important in mammalian bioprocesses at the manufacturing scale in order to ensure robust cell growth, high protein yields, and consistent quality attributes. The majority of bioprocess development
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::c5e4f1e68631d77235ae36d186f01486
https://pubmed.ncbi.nlm.nih.gov/27922179
https://pubmed.ncbi.nlm.nih.gov/27922179
Autor:
Somak Ray, Yuanwei Gao, Michael C. Borys, Amanda M. Lewis, Barry L. Karger, Shujia Dai, Zhuangrong Huang, Zizhuo Xing, Nicholas R. Abu-Absi, Zheng Jian Li, Alexander R. Ivanov
Publikováno v:
Biotechnology journal. 11(9)
Large-scale bioprocessing is key to the successful manufacturing of a biopharmaceutical. However, cell viability and productivity are often lower in the scale-up from laboratory to production. In this study, we analyzed CHO cells, which showed lower
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1dda16dea41912a19c70615dd1ae896e
https://pubmed.ncbi.nlm.nih.gov/27351837
https://pubmed.ncbi.nlm.nih.gov/27351837
Autor:
David S. Meyers, Li You, Zizhuo Xing, Yueming Qian, Zheng Jian Li, Paul M. Skonezny, Stankavage Robert, Jianlin Xu, Shih-Hsie Pan
Publikováno v:
Journal of Industrial Microbiology and Biotechnology. 39:1199-1208
A standard fed-batch fermentation process using 1 mM isopropyl-β-d-thiogalactopyranoside (IPTG) induction at 37 °C in complex batch and feed media had been developed for manufacturing of a therapeutic protein (TP) expressed in inclusion bodies (IBs
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2139a690f2b21c0d1f2bcd16e13312b3
https://doi.org/10.1007/s10295-012-1127-8
https://doi.org/10.1007/s10295-012-1127-8
Publikováno v:
Process Biochemistry. 46:1423-1429
The amino acid composition of CHO cell culture media for manufacturing an antibody fusion protein (B1) was optimized using data obtained through metabolic flux analysis. The metabolic flux analysis was performed using the metabolic rates derived from
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::617150030c9ce3096758446c32a3c621
https://doi.org/10.1016/j.procbio.2011.03.014
https://doi.org/10.1016/j.procbio.2011.03.014
Publikováno v:
Biotechnology Progress. 24:675-683
Elevation of lactate, ammonia, osmolality, and carbon dioxide to inhibitory levels was reported to have adverse effects on cell growth and protein productivity in mammalian cell culture. Multivariate analysis methods were used to investigate the role
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::85c245fda77f31bd5e1519f344cf9a91
https://doi.org/10.1021/bp070466m
https://doi.org/10.1021/bp070466m
Publikováno v:
HortScience. 40:1464-1468
A successful tissue culture initiation step often begins with effective explant sterilization. To improve douglas fir bud culture initiation, five sterilization treatments (20% bleach, 100% bleach, 3 second flaming, 5 second flaming, and self-extingu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::20a1868c21ef71651661e7099c975f9c
https://doi.org/10.21273/hortsci.40.5.1464
https://doi.org/10.21273/hortsci.40.5.1464
Autor:
Sherry Lee, Zizhuo Xing, Paul S. Kayne, Nancy A. Mackin, Aiqing He, Nan-xin Qian, Zheng Jian Li, Yueming Qian, Qin He
Publikováno v:
Biotechnology journal. 9(11)
Shake flasks and bench-top bioreactors are widely used for cell culture process development, however, culture performances significantly differ between them. In order to apply the results received from small-scale cultures to production scale, it is
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5e680b6e69313c23d15508bcfe0d1add
https://pubmed.ncbi.nlm.nih.gov/25271019
https://pubmed.ncbi.nlm.nih.gov/25271019