High-efficiency purification of divergent AAV serotypes using AAVX affinity chromatography

Autor: Florea, Michael, Nicolaou, Fotini, Pacouret, Simon, Zinn, Eric M., Sanmiguel, Julio, Andres-Mateos, Eva, Unzu, Carmen, Wagers, Amy J., Vandenberghe, Luk H.

Publikováno v: In Molecular Therapy - Methods & Clinical Development 9 March 2023 28:146-159

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High-efficiency purification of divergent AAV serotypes using AAVX affinity chromatography.

Autor: Florea M; Grousbeck Gene Therapy Center, Schepens Eye Research Institute and Massachusetts Eye and Ear Infirmary, Boston, MA, USA.; Harvard Ph.D. Program in Biological and Biomedical Sciences, Division of Medical Sciences, Harvard University, Boston, MA, USA.; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA., Nicolaou F; Grousbeck Gene Therapy Center, Schepens Eye Research Institute and Massachusetts Eye and Ear Infirmary, Boston, MA, USA., Pacouret S; Grousbeck Gene Therapy Center, Schepens Eye Research Institute and Massachusetts Eye and Ear Infirmary, Boston, MA, USA., Zinn EM; Grousbeck Gene Therapy Center, Schepens Eye Research Institute and Massachusetts Eye and Ear Infirmary, Boston, MA, USA., Sanmiguel J; Grousbeck Gene Therapy Center, Schepens Eye Research Institute and Massachusetts Eye and Ear Infirmary, Boston, MA, USA., Andres-Mateos E; Grousbeck Gene Therapy Center, Schepens Eye Research Institute and Massachusetts Eye and Ear Infirmary, Boston, MA, USA., Unzu C; Grousbeck Gene Therapy Center, Schepens Eye Research Institute and Massachusetts Eye and Ear Infirmary, Boston, MA, USA., Wagers AJ; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.; Harvard Stem Cell Institute, Harvard University, Cambridge, MA, USA.; Paul F. Glenn Center for the Biology of Aging, Harvard Medical School, Boston, MA, USA.; Section on Islet Cell and Regenerative Biology, Joslin Diabetes Center, Boston, MA, USA., Vandenberghe LH; Grousbeck Gene Therapy Center, Schepens Eye Research Institute and Massachusetts Eye and Ear Infirmary, Boston, MA, USA.; Ocular Genomics Institute, Department of Ophthalmology, Harvard Medical School, Boston, MA, USA.; The Broad Institute of Harvard and MIT, Boston, MA, USA.; Harvard Stem Cell Institute, Harvard University, Cambridge, MA, USA.

Publikováno v: Molecular therapy. Methods & clinical development [Mol Ther Methods Clin Dev] 2022 Dec 16; Vol. 28, pp. 146-159. Date of Electronic Publication: 2022 Dec 16 (Print Publication: 2023).