Zobrazeno 1 - 10
of 18
pro vyhledávání: '"Zhong-Yao Shen"'
Publikováno v:
In Journal of Chromatography A 21 January 2011 1218(3):524-533
Publikováno v:
Journal of Microbiology and Biotechnology. 26:337-346
Three combinations of molecular chaperones from Escherichia coli (i.e., DnaK-DnaJ-GrpEGroEL- GroES, GroEL-GroES, and DnaK-DnaJ-GrpE) were overproduced in E. coli BL21, and their in vitro stabilizing effects on a nitrile hydratase (NHase) were assesse
Publikováno v:
Spectroscopy Letters. Mar2005, Vol. 38 Issue 2, p195-208. 14p. 3 Charts.
Autor:
Hui Min Yu, Yan Hong Chang, Kai Hua Han, Hui Luo, Yao Zhen Xie, Shun Yao, Qiang Li, Zhong Yao Shen
Publikováno v:
Advanced Materials Research. :682-688
Some kinds of epoxy supports, LX1000-EP(C), LX1000-EP(D), LKZ-116, LKZ-118 and LKZ-126 were utilized to covalently immobilize cephalosporin C (CPC) acylase, the key enzyme in the one-step enzymatic process of 7-aminocephalosporanic acid (7-ACA) produ
Publikováno v:
Carbohydrate Research. 201:241-248
The reactions of cyclomaltoheptaose (β-cyclodextrin) and maleic anhydride were investigated, and the resulting products characterized by i.r., proton, and carbon-13 n.m.r. spectroscopy. The 13 C-n.m.r. spectra show preferential esterification at HO-
Publikováno v:
Sheng wu gong cheng xue bao = Chinese journal of biotechnology. 22(4)
The amidase of Nocardia sp. is one of important industrial enzymes. Based on DNA and protein sequence alignment from different strains, a new gene of amidase was successfully cloned from Nocardia YS-2002, which is widely used for industrial productio
Publikováno v:
Wei sheng wu xue bao = Acta microbiologica Sinica. 45(6)
Heterogenous expression of active nitrile hydratase (NHase) was focused for its great potential in genetically evolution of the operational stability of NHase. Two recombinant Escherichia coli strains, E. coli JM105 (pUC18-NHBAX) and E. coli BL21 (DE
Publikováno v:
Sheng wu gong cheng xue bao = Chinese journal of biotechnology. 20(3)
Industrial biocatalysis is currently attracting much attention to rebuild or substitute traditional producing process of chemicals and drugs. One of key focuses in industrial biocatalysis is biocatalyst, which is usually one kind of microbial enzyme.
Publikováno v:
Sheng wu gong cheng xue bao = Chinese journal of biotechnology. 18(2)
The hydration reaction by microbial method is the crisis of the procedure of acrylamide production from acrylonitrile. This research studied the enzyme catalytic kinetics and de-active kinetics of nitrile hydratase in the type of free cell. Firstly,
Publikováno v:
Sheng wu gong cheng xue bao = Chinese journal of biotechnology. 18(1)
The cultural conditions for the growth of Norcardia cell were studied in this paper. Controlling pH value, adding nutrient and optimizing the quantity of inducer during cultivation, the activity of nitrile hydratase reached 6567 u/mL (culture medium)