Zobrazeno 1 - 10
of 127
pro vyhledávání: '"Zhike Lu"'
Publikováno v:
Advanced Science, Vol 11, Iss 21, Pp n/a-n/a (2024)
Abstract Wild‐type sortase A is an important virulence factor displaying a diverse array of proteins on the surface of bacteria. This protein display relies on the transpeptidase activity of sortase A, which is widely engineered to allow protein li
Externí odkaz:
https://doaj.org/article/ac7c996410f34a9eb5989022a0deeaef
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-10 (2023)
Abstract Genome-wide clustered regularly interspaced short palindromic repeats (CRISPR)-based knockout screening is revolting the genetic analysis of a cellular or molecular phenotype in question but is challenged by the large size of single-guide RN
Externí odkaz:
https://doaj.org/article/5077d345502647e88b094a61c32c87fc
Autor:
Jianbo Li, Panfeng Wu, Zhoutao Cao, Guanlan Huang, Zhike Lu, Jianfeng Yan, Heng Zhang, Yangfan Zhou, Rong Liu, Hui Chen, Lijia Ma, Mengcheng Luo
Publikováno v:
Cell Reports, Vol 43, Iss 2, Pp 113765- (2024)
Summary: The increasing emergence of Cas9 variants has attracted broad interest, as these variants were designed to expand CRISPR applications. New Cas9 variants typically feature higher editing efficiency, improved editing specificity, or alternativ
Externí odkaz:
https://doaj.org/article/c2ab74585f1c42a3b0819013104c6643
Autor:
Heng Zhang, Jianfeng Yan, Zhike Lu, Yangfan Zhou, Qingfeng Zhang, Tingting Cui, Yini Li, Hui Chen, Lijia Ma
Publikováno v:
Cell Discovery, Vol 9, Iss 1, Pp 1-20 (2023)
Abstract Life science studies involving clustered regularly interspaced short palindromic repeat (CRISPR) editing generally apply the best-performing guide RNA (gRNA) for a gene of interest. Computational models are combined with massive experimental
Externí odkaz:
https://doaj.org/article/d881e6e86c01463aa5b6f0f3696e4ad5
Autor:
Zhenxing Yu, Zhike Lu, Jingjing Li, Yingying Wang, Panfeng Wu, Yini Li, Yangfan Zhou, Bailun Li, Heng Zhang, Yingzheng Liu, Lijia Ma
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-13 (2022)
It is still a challenge to accurately identify off-target endonuclease edits. Here the authors report PEAC-seq using a Prime Editor to insert a tag to the editing sites and enrich the tagged regions with site-specific primers for sequencing: they sho
Externí odkaz:
https://doaj.org/article/906145db752d453cb2223b7e2782e62c
Autor:
Yang Liu, Renjie Song, Lu Zhao, Zhike Lu, Yini Li, Xinyi Zhan, Fengjiao Lu, Jiang Yang, Yamei Niu, Xuetao Cao
Publikováno v:
Signal Transduction and Targeted Therapy, Vol 7, Iss 1, Pp 1-15 (2022)
Abstract Neutrophil migration into the site of infection is necessary for antibacterial innate defense, whereas impaired neutrophil migration may result in excessive inflammation and even sepsis. The neutrophil migration directed by extracellular sig
Externí odkaz:
https://doaj.org/article/bf2fb281bd7c4c6084b70755bf471c8b
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-1 (2023)
Externí odkaz:
https://doaj.org/article/164a074c1c5a4911b6791668618d2c26
Autor:
Qingkai Song, Ke Ni, Min Liu, Yini Li, Lixia Wang, Yingying Wang, Yingzheng Liu, Zhenxing Yu, Yinyao Qi, Zhike Lu, Lijia Ma
Publikováno v:
Genome Biology, Vol 21, Iss 1, Pp 1-15 (2020)
Abstract CRISPR-based genome perturbation provides a new avenue to conveniently change DNA sequences, transcription, and epigenetic modifications in genetic screens. However, it remains challenging to assay the complex molecular readouts after pertur
Externí odkaz:
https://doaj.org/article/dda584ede1c94f3e9e80cb55f11bb7ed
Autor:
Jiajie Hou, He Zhang, Jun Liu, Zhenjun Zhao, Jianye Wang, Zhike Lu, Bian Hu, Jiankui Zhou, Zhicong Zhao, Mingxuan Feng, Haiyan Zhang, Bin Shen, Xingxu Huang, Beicheng Sun, Chuan He, Qiang Xia
Publikováno v:
Molecular Cancer, Vol 18, Iss 1, Pp 1-17 (2019)
Abstract Background Dynamic N 6-methyladenosine (m6A) modification was previously identified as a ubiquitous post-transcriptional regulation that affected mRNA homeostasis. However, the m6A-related epitranscriptomic alterations and functions remain e
Externí odkaz:
https://doaj.org/article/d5318f7dcb2c43dfac840d8ed9c6e91d
Autor:
Miaoge Xue, Boxuan Simen Zhao, Zijie Zhang, Mijia Lu, Olivia Harder, Phylip Chen, Zhike Lu, Anzhong Li, Yuanmei Ma, Yunsheng Xu, Xueya Liang, Jiyong Zhou, Stefan Niewiesk, Mark E. Peeples, Chuan He, Jianrong Li
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-18 (2019)
Here, Xue et al. identify N 6-methyladenosine (m6A) modification sites in RNAs of respiratory syncytial virus (RSV) and show that these sites, particularly sites in the transcript encoding for the viral glycoprotein, affect virus replication in prima
Externí odkaz:
https://doaj.org/article/ae5353928f864471a17a92fe4c1248b7