Zobrazeno 1 - 10
of 40
pro vyhledávání: '"Zhi-Xue Yang"'
Autor:
Zhi-Xue Yang, Dong-Hao Deng, Zhu-Ying Gao, Zhi-Kang Zhang, Ya-Wen Fu, Wei Wen, Feng Zhang, Xiang Li, Hua-Yu Li, Jian-Ping Zhang, Xiao-Bing Zhang
Publikováno v:
Communications Biology, Vol 7, Iss 1, Pp 1-15 (2024)
Abstract The potential for off-target mutations is a critical concern for the therapeutic application of CRISPR-Cas9 gene editing. Current detection methodologies, such as GUIDE-seq, exhibit limitations in oligonucleotide integration efficiency and s
Externí odkaz:
https://doaj.org/article/ca2357b3dbc94140bda92191948be5cd
Autor:
Juan-Juan Zhao, Xin-Yu Sun, Sai-Ning Tian, Zong-Ze Zhao, Meng-Di Yin, Mei Zhao, Feng Zhang, Si-Ang Li, Zhi-Xue Yang, Wei Wen, Tao Cheng, An Gong, Jian-Ping Zhang, Xiao-Bing Zhang
Publikováno v:
BMC Genomics, Vol 25, Iss 1, Pp 1-23 (2024)
Abstract Background CRISPR-Cas9 technology has advanced in vivo gene therapy for disorders like hemophilia A, notably through the successful targeted incorporation of the F8 gene into the Alb locus in hepatocytes, effectively curing this disorder in
Externí odkaz:
https://doaj.org/article/6b00ee3c16a94b60a7273e094f61df57
Autor:
Zi-Jun Quan, Si-Ang Li, Zhi-Xue Yang, Juan-Juan Zhao, Guo-Hua Li, Feng Zhang, Wei Wen, Tao Cheng, Xiao-Bing Zhang
Publikováno v:
Genomics, Proteomics & Bioinformatics, Vol 21, Iss 6, Pp 1221-1236 (2023)
To achieve the enormous potential of gene-editing technology in clinical therapies, one needs to evaluate both the on-target efficiency and unintended editing consequences comprehensively. However, there is a lack of a pipelined, large-scale, and eco
Externí odkaz:
https://doaj.org/article/420cff9498ce4ae0b14827b32d3370f6
Autor:
Zhi-Xue Yang, Ya-Wen Fu, Juan-Juan Zhao, Feng Zhang, Si-Ang Li, Mei Zhao, Wei Wen, Lei Zhang, Tao Cheng, Jian-Ping Zhang, Xiao-Bing Zhang
Publikováno v:
Genomics, Proteomics & Bioinformatics, Vol 21, Iss 6, Pp 1206-1220 (2023)
A series of clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated protein 9 (Cas9) systems have been engineered for genome editing. The most widely used Cas9 is SpCas9 from Streptococcus pyogenes and SaCas9 from Staphyl
Externí odkaz:
https://doaj.org/article/dd9a845d89064b8db6bec0665d9eb342
Autor:
Wanqiu Chen, Chenguang Wang, Zhi-Xue Yang, Feng Zhang, Wei Wen, Christoph Schaniel, Xianqiang Mi, Matthew Bock, Xiao-Bing Zhang, Hongyu Qiu, Charles Wang
Publikováno v:
Communications Biology, Vol 6, Iss 1, Pp 1-14 (2023)
Abstract Mesenchymal stromal cells (MSCs) have great value in cell therapies. The MSC therapies have many challenges due to its inconsistent potency and limited quantity. Here, we report a strategy to generate induced MSCs (iMSCs) by directly reprogr
Externí odkaz:
https://doaj.org/article/25f5c7eae5b444eebad0fb5a895d2aa6
Autor:
Wei Wen, Zi-Jun Quan, Si-Ang Li, Zhi-Xue Yang, Ya-Wen Fu, Feng Zhang, Guo-Hua Li, Mei Zhao, Meng-Di Yin, Jing Xu, Jian-Ping Zhang, Tao Cheng, Xiao-Bing Zhang
Publikováno v:
Genome Biology, Vol 22, Iss 1, Pp 1-22 (2021)
Abstract Background After repairing double-strand breaks (DSBs) caused by CRISPR-Cas9 cleavage, genomic damage, such as large deletions, may have pathogenic consequences. Results We show that large deletions are ubiquitous but are dependent on editin
Externí odkaz:
https://doaj.org/article/df51a80f1e864427bde4af68b40acd79
Autor:
Jian-Ping Zhang, Xin-Xin Cheng, Mei Zhao, Guo-Hua Li, Jing Xu, Feng Zhang, Meng-Di Yin, Fei-Ying Meng, Xin-Yue Dai, Ya-Wen Fu, Zhi-Xue Yang, Cameron Arakaki, Ruijun Jeanna Su, Wei Wen, Wen-Tian Wang, Wanqiu Chen, Hannah Choi, Charles Wang, Guangping Gao, Lei Zhang, Tao Cheng, Xiao-Bing Zhang
Publikováno v:
Genome Biology, Vol 20, Iss 1, Pp 1-17 (2019)
Abstract Background Hemophilia A, a bleeding disorder resulting from F8 mutations, can only be cured by gene therapy. A promising strategy is CRISPR-Cas9-mediated precise insertion of F8 in hepatocytes at highly expressed gene loci, such as albumin (
Externí odkaz:
https://doaj.org/article/0175546634cc4b5da1856d314090ad7d
Autor:
Zhi-Xue Yang, Bo Zhang, Jinrong Wei, Guo-Qin Jiang, Yan-Lin Wu, Bing-Jing Leng, Chun-Gen Xing
Publikováno v:
Cancer Cell International, Vol 18, Iss 1, Pp 1-10 (2018)
Abstract Background Recent studies have shown that laminin subunit alpha 4 (LAMA4) plays an important role in carcinogenesis. However, its molecular biological function in triple-negative breast cancer (TNBC) has not been entirely clarified. This stu
Externí odkaz:
https://doaj.org/article/d75b1361cd844ec6b2763c778edce145
Autor:
Juan Fu, Ya-Wen Fu, Juan-Juan Zhao, Zhi-Xue Yang, Si-Ang Li, Guo-Hua Li, Zi-Jun Quan, Feng Zhang, Jian-Ping Zhang, Xiao-Bing Zhang, Chang-Kai Sun
Publikováno v:
Stem Cell Reviews and Reports. 18:1822-1833
Highly efficient gene knockout (KO) editing of CRISPR–Cas9 has been achieved in iPSCs, whereas homology-directed repair (HDR)-mediated precise gene knock-in (KI) and high-level expression are still bottlenecks for the clinical applications of iPSCs
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9049a885066256d78deaf31a0e687d6f
https://doi.org/10.1007/s12015-022-10331-1
https://doi.org/10.1007/s12015-022-10331-1
Autor:
Jing Xu, Mei Zhao, Wei Wen, Meng-Di Yin, Zi-Jun Quan, Tao Cheng, Ya-Wen Fu, Guo-Hua Li, Feng Zhang, Xiao-Bing Zhang, Zhi-Xue Yang, Jian-Ping Zhang, Si-Ang Li
Publikováno v:
Genome Biology, Vol 22, Iss 1, Pp 1-22 (2021)
Genome Biology
Genome Biology
BackgroundAfter repairing double-strand breaks (DSBs) caused by CRISPR-Cas9 cleavage, genomic damage, such as large deletions, may have pathogenic consequences.ResultsWe show that large deletions are ubiquitous but are dependent on editing sites and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a207dbedddc97e3343e53f19f85f6a9
https://doaj.org/article/df51a80f1e864427bde4af68b40acd79
https://doaj.org/article/df51a80f1e864427bde4af68b40acd79