A simple and rapid protein purification method based on cell-surface display of SUMO-fused recombinant protein and Ulp1 protease

Autor: Xiao-Feng Zhou, Chen-Lu Zhang, Xue-Ping Gao, Wei-Long Wang, Zheng-Fen He, Feng-Ying Jiang, Yi-Lin Pang, Jiang-Hui Li, Xiao-Jun Ren, Huai-Bin Zhou, Guo-Qiang Tan, Jian-Xin Lyu, Wu Wang

Publikováno v: AMB Express, Vol 10, Iss 1, Pp 1-9 (2020)

Abstract The development of novel methods for highly efficient protein purification remains a research focus in the biotechnology field because conventional purification approaches, including affinity purification, gel filtration, and ion-exchange ch

Externí odkaz: https://doaj.org/article/cec37ba64ef0410a97c162892f3177bc

A simple and rapid protein purification method based on cell-surface display of SUMO-fused recombinant protein and Ulp1 protease.

Autor: Zhou, Xiao-Feng¹ (AUTHOR), Zhang, Chen-Lu¹ (AUTHOR), Gao, Xue-Ping¹ (AUTHOR), Wang, Wei-Long¹ (AUTHOR), He, Zheng-Fen¹ (AUTHOR), Jiang, Feng-Ying² (AUTHOR), Pang, Yi-Lin¹ (AUTHOR), Li, Jiang-Hui¹ (AUTHOR), Ren, Xiao-Jun¹ (AUTHOR), Zhou, Huai-Bin¹ (AUTHOR), Tan, Guo-Qiang¹ (AUTHOR) tgq@wmu.edu.cn, Lyu, Jian-Xin¹ (AUTHOR) jxlu313@163.com, Wang, Wu¹ (AUTHOR) wangwu@wmu.edu.cn

Publikováno v: AMB Express. 4/7/2020, Vol. 10 Issue 1, p1-9. 9p.