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Real-time and simultaneous assay of monophenolase and diphenolase activity in tyrosinase catalyzed cascade reactions by combination of three-way calibration and excitation-emission matrix fluorescence

Autor: Ling Zhang, Qi Shang, Yuanze Zhao, Zhaoqi Ran, Chan Chen, Weikang Tang, Wenbin Liu

Publikováno v: Analytical and Bioanalytical Chemistry. 414:2439-2452

A real-time assay for multiple enzyme activities in cascade reactions is required for research on metabolism and bioengineering. Tyrosinase has the bifunctional activity of monophenolase and diphenolase. A combined strategy of three-way calibration w

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::495d463f67ef7fb95dd3bba753930ee0
https://doi.org/10.1007/s00216-022-03884-0

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First derivative synchronous fluorometric method to continuously measure monophenolase activity

Autor: Weikang Tang, Xiawen Wang, Ling Zhang, Wenbin Liu, Zhaoqi Ran, Qi Shang, Chan Chen, Jiaze Li

Publikováno v: Enzyme and microbial technology. 150

Tyrosinase plays an essential role in melanin biosynthesis and inherently exhibits both monophenolase and diphenolase activity. A first derivative synchronous fluorometric assay was established for directly monitoring monophenolase activity. The zero

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::71bc0e7dfe266ec34422d71521be36e0
https://pubmed.ncbi.nlm.nih.gov/34489037

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