Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Zhanna Hakhverdyan"'
Autor:
Zhanna Hakhverdyan, Kelly R. Molloy, Roman I. Subbotin, Javier Fernandez-Martinez, Brian T. Chait, Michael P. Rout
Publikováno v:
STAR Protocols, Vol 2, Iss 3, Pp 100800- (2021)
Summary: We present a comprehensive and robust protocol to track the dynamics of all proteins in a complex in yeast cells. A single member of the protein assembly is tagged and conditionally expressed, minimizing the perturbations to the protein comp
Externí odkaz:
https://doaj.org/article/8f14c84eb3e346c589f3e578b975f3cc
Autor:
Zhanna Hakhverdyan, David J Thaller, Sapan Borah, Megan C. King, Elisa C. Rodriguez, Anthony W Isenhour, C. Patrick Lusk, Michael P. Rout
Publikováno v:
Molecular Biology of the Cell
Integral membrane proteins of the Lap2-emerin-MAN1 (LEM) family have emerged as important components of the inner nuclear membrane (INM) required for the functional and physical integrity of the nuclear envelope. However, like many INM proteins, ther
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fd6bc7fa4775e1b7fb6420b9a24e1f11
https://doi.org/10.1091/mbc.e20-09-0584
https://doi.org/10.1091/mbc.e20-09-0584
Autor:
Sapan Borah, C. Patrick Lusk, Zhanna Hakhverdyan, David J Thaller, Megan C. King, Elisa C. Rodriguez, Michael P. Rout
Integral membrane proteins of the Lap2-emerin-MAN1 (LEM) family have emerged as important components of the inner nuclear membrane (INM) required for the functional and physical integrity of the nuclear envelope. However, like many INM proteins, ther
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5ae39639b2e66fc58fb8fa8b3a88219f
https://doi.org/10.1101/2020.06.29.178129
https://doi.org/10.1101/2020.06.29.178129
Autor:
Sarah Keegan, Javier Fernandez-Martinez, Brian T. Chait, John D. Aitchison, David Fenyö, Michael P. Rout, Kelly R. Molloy, Dante M. Lepore, Thurston Herricks, Mary Munson, Zhanna Hakhverdyan, Roman I. Subbotin
Publikováno v:
Mol Cell
Cellular processes are largely carried out by macromolecular assemblies, most of which are dynamic, having components that are in constant flux. One such assembly is the nuclear pore complex (NPC), an ∼50 MDa assembly comprised of ∼30 different p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b165d8bc55d9f55153f15647fba4e2e9
https://pubmed.ncbi.nlm.nih.gov/33333016
https://pubmed.ncbi.nlm.nih.gov/33333016
Autor:
Brian T. Chait, Dante M. Lepore, Mary Munson, Roman I. Subbotin, Kelly R. Molloy, Javier Fernandez-Martinez, Zhanna Hakhverdyan, Michael P. Rout, Sarah Keegan, David Fenyö
Publikováno v:
SSRN Electronic Journal.
Cellular processes are largely carried out by macromolecular assemblies, most of which are dynamic, having components that are in constant flux. One such assembly is the nuclear pore complex (NPC), a ~50 MDa assembly comprised of ~30 different protei
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d41443bfc606034b102e88066701a81f
https://doi.org/10.2139/ssrn.3584516
https://doi.org/10.2139/ssrn.3584516
Publikováno v:
Cold Spring Harbor protocols. 2016(7)
Here, we describe an affinity isolation protocol. It uses cryomilled yeast cell powder for producing cell extracts and antibody-conjugated paramagnetic beads for affinity capture. Guidelines for determining the optimal extraction solvent composition
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::932447255e814c2a29d547fc09b63e12
https://pubmed.ncbi.nlm.nih.gov/27371596
https://pubmed.ncbi.nlm.nih.gov/27371596
Autor:
Nicholas Farrall, Mary Munson, Caroline M. Duffy, Michael P. Rout, Zhanna Hakhverdyan, Anne M. Mirza, Margaret R. Heider, Mark C. Field, Alexandra C. Walls, Mingyu Gu, Laura L Marcotte, Adam Frost
Publikováno v:
Nature structuralmolecular biology. 23(1)
The exocyst is a hetero-octameric complex that has been proposed to serve as the tethering complex for exocytosis, although it remains poorly understood at the molecular level. Here, we purified endogenous exocyst complexes from Saccharomyces cerevis
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e7b83fba1bd7df79767b52f9b521aa91
https://pubmed.ncbi.nlm.nih.gov/26656853
https://pubmed.ncbi.nlm.nih.gov/26656853
Publikováno v:
Cold Spring Harbor Protocols. 2016:pdb.top077545
Affinity capture has become a powerful technique for consistently purifying endogenous protein complexes, facilitating biochemical and biophysical assays on otherwise inaccessible biological assemblies, and enabling broader interactomic exploration.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::557bd9cbb4a8baaac619843efe853161
https://doi.org/10.1101/pdb.top077545
https://doi.org/10.1101/pdb.top077545
Autor:
Zhanna Hakhverdyan, Brian T. Chait, Anil Oroskar, Loren E. Hough, Asha A. Oroskar, Michael P. Rout, Michal Domanski, John LaCava, Torben Heick Jensen, David Fenyö
Publikováno v:
The FASEB Journal. 26
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b4a79e00f741a21131da6e327c46b8d2
https://doi.org/10.1096/fasebj.26.1_supplement.776.2
https://doi.org/10.1096/fasebj.26.1_supplement.776.2