Výsledky vyhledávání - "Zdeněk Hostomský"

M13 and pUC vectors with new unique restriction sites for cloning

Autor: Zdeněk Hostomský, Vladimir Benes, Václav Pačes, Luboš Arnold

Publikováno v: Gene. 130:151-152

Several vectors based on the widely used phage M13 and plasmid pUC were constructed. The vectors contain polylinkers (MCS) for DNA insertions with several new restriction sites (e.g., ApaI, NotI, StuI, SacII). Moreover, the NarI site in the nonessent

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::108ee6b9fd568446c3afebe91d47cea7
https://doi.org/10.1016/0378-1119(93)90360-f

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Synthesis of oligodeoxyribonucleotides using in situ prepared nucleoside methyl phosphochloridite intermediates

Autor: Jiří Smrt, Zdeněk Hostomský, André Rosenthal, J. Forstová, Hana Večerková

Publikováno v: Collection of Czechoslovak Chemical Communications. 50:2201-2209

Synthesis of oligodeoxyribonucleotides on silica gel support using 5'-O-dimethoxytrityl-(N-acyl)-deoxyribonucleoside 3'-(O-methyl phosphochloridite) intermediates (I) is described. Solutions of these intermediates were prepared before use by mixing o

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::5df164cb2cb6ea9b89afb06fdf6ae6d9
https://doi.org/10.1135/cccc19852201

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Inhibition by dihydrorifampicin of RNA polymerases I and II isolated from nuclei of Rous sarcoma cells and human tumor cells HEp-2

Autor: Jindřich Kára, Zdeněk Hostomský

Publikováno v: Collection of Czechoslovak Chemical Communications. 44:2722-2736

Dihydrorifampicin, a rifampicin derivative hydrogenated at the 18-19 carbon atoms of the aliphatic ansa chain of the rifampicin molecule, inhibits the enzymatic activity of RNA polymerases I and II, isolated from the nuclei of avian tumor cells (Rous

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::c711cef8760eb3735afd26cdcdb92060
https://doi.org/10.1135/cccc19792722

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Automated chloridite and amidite synthesis of oligodeoxyribonucleotides on a long chain support using amidine protected purine nucleosides

Autor: Václav Pačes, Luboš Arnold, Eliška Bradková, Zdeněk Hostomský, Zdeněk Točík, Jiří Smrt

Publikováno v: Collection of Czechoslovak Chemical Communications. 54:523-532

Preparation of solid supports with long aliphatic spacer for the oligonucleotide synthesis is described. Automated synthesis of oligodeoxyribonucleotides using in situ prepared methylchlorophosphite intermediates from amidine protected purine nucleos

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::8b3eab598542b3087592b71b34046717
https://doi.org/10.1135/cccc19890523

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Comparison of genomes of closely related phages phi 29, phi 15 and PZA using a rapid method of parallel physical mapping

Autor: Václav Pačes, Zdeněk Hostomský, Stanislav Zadražil

Publikováno v: FEBS letters. 188(1)

The DNAs of phages o29, o15 and PZA of Bacillus subtilis were analysed with restriction enzymes Eco RI, Hpa I and Hin dIII. A method was used which permits parallel physical mapping of all three phages, from both ends of their linear genomes. The met

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d5dde296ad46369caa7d71b87bed935c
https://pubmed.ncbi.nlm.nih.gov/2991017

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Nucleotide sequence of the right early region of Bacillus subtilis phage PZA completes the 19366-bp sequence of PZA genome. Comparison with the homologous sequence of phage phi 29

Autor: Zdeněk Hostomský, Václav Pačes, Pavel Urbánek, Čestmír Vlček

Publikováno v: Gene. 44(1)

We have sequenced the rightmost 2079 bp of the Bacillus subtilis phage PZA genome. This region encompasses the right early region. We compared it with the homologous region of phage φ29. Six open reading frames (ORFs) were found in this region of PZ

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c3416e635ad5b528bca57b6434b6cff
https://pubmed.ncbi.nlm.nih.gov/3095189

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Transcriptional and Translational Signals in Phages PZA and ø29

Autor: Zdeněk Hostomský, Pavel Urbánek, Václav Pačes, Stanislav Zadražil, Čestmír Vlček

Publikováno v: Gene Manipulation and Expression ISBN: 9789401165679

There is a group of small and morphologically similar bacteriophages that lytically infect cells of Bacillus subtilis (1,2). This report is concerned with two of them, namely PZA and o29. They both contain linear, duplex DNA, 18 kb long. Genomes of t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::4f166342f2e1bd5a329ef05868a39f93
https://doi.org/10.1007/978-94-011-6565-5_16

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Solid-phase assembly of cow colostrum trypsin inhibitor gene

Autor: Zdeněk Točík, Jiří Smrt, Zdeněk Hostomský, Václav Pačes, Luboš Arnold

A gene for cow colostrum trypsin inhibitor (CTI) was constructed from synthetic oligonucleotides using a novel method of solid-phase gene assembly. In the first step an anchor oligonucleotide was covalently bound to the CNBr-activated Sephacryl S-500

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3a9b687e2e0dc1d07abd6249f21d4aef
https://europepmc.org/articles/PMC305922/

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