Zobrazeno 1 - 10
of 24
pro vyhledávání: '"Zdeněk, Kukačka"'
Autor:
Pieter-Jan Vermeire, Anastasia V. Lilina, Hani M. Hashim, Lada Dlabolová, Jan Fiala, Steven Beelen, Zdeněk Kukačka, Jeremy N. Harvey, Petr Novák, Sergei V. Strelkov
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-1 (2024)
Externí odkaz:
https://doaj.org/article/133c3e35d17b4d598dd6ede16ac5ccd5
Autor:
Pieter-Jan Vermeire, Anastasia V. Lilina, Hani M. Hashim, Lada Dlabolová, Jan Fiala, Steven Beelen, Zdeněk Kukačka, Jeremy N. Harvey, Petr Novák, Sergei V. Strelkov
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-16 (2023)
Abstract Intermediate filaments (IFs) are essential constituents of the metazoan cytoskeleton. A vast family of cytoplasmic IF proteins are capable of self-assembly from soluble tetrameric species into typical 10–12 nm wide filaments. The primary s
Externí odkaz:
https://doaj.org/article/4815cc6da527458d859f7d1766b80d15
Autor:
Ghazaleh Yassaghi, Zdeněk Kukačka, Jan Fiala, Daniel Kavan, Petr Halada, Michael Volný, Petr Novák
Publikováno v:
Analytical Chemistry
Fast photochemical oxidation of proteins (FPOP) footprinting is a structural mass spectrometry method that maps proteins by fast and irreversible chemical reactions. The position of oxidative modification reflects solvent accessibility and site react
Autor:
Lukáš Slavata, Josef Chmelík, Daniel Kavan, Růžena Filandrová, Jan Fiala, Michal Rosůlek, Hynek Mrázek, Zdeněk Kukačka, Karel Vališ, Petr Man, Michael Miller, William McIntyre, Daniele Fabris, Petr Novák
Publikováno v:
Biomolecules, Vol 9, Iss 10, p 535 (2019)
The limited information available on the structure of complexes involving transcription factors and cognate DNA response elements represents a major obstacle in the quest to understand their mechanism of action at the molecular level. We implemented
Externí odkaz:
https://doaj.org/article/0ec5db4b1ea44b2796123325e7b7363f
Autor:
Marek Polák, Ghazaleh Yassaghi, Daniel Kavan, František Filandr, Jan Fiala, Zdeněk Kukačka, Petr Halada, Dmitry S. Loginov, Petr Novák
Publikováno v:
Analytical chemistry. 94(7)
A combination of covalent labeling techniques and mass spectrometry (MS) is currently a progressive approach for deriving insights related to the mapping of protein surfaces or protein-ligand interactions. In this study, we mapped an interaction inte
Autor:
Zdeněk Kukačka, Lukáš Fojtík, Petr Pompach, Jan Fiala, Petr Beier, Josef Chmelík, Petr Novák, Václav Matoušek
Publikováno v:
J.Am.Chem. Soc.
Covalent labeling of proteins in combination with mass spectrometry has been established as a complementary technique to classical structural methods, such as X-ray, NMR, or cryogenic electron microscopy (Cryo-EM), used for protein structure determin
Autor:
Roland Beckmann, Thanh Binh Nguyen, Zdeněk Kukačka, Amanda K. Chaplin, Aleš Hnízda, Petr Novák, Petr Tesina, Lukas Kater, Tom L. Blundell, David B. Ascher
Publikováno v:
The Febs Journal
Funder: Victorian Government
Funder: BBSRC
Nonhomologous end joining (NHEJ) is a DNA repair mechanism that religates double-strand DNA breaks to maintain genomic integrity during the entire cell cycle. The Ku70/80 complex recognizes DNA bre
Funder: BBSRC
Nonhomologous end joining (NHEJ) is a DNA repair mechanism that religates double-strand DNA breaks to maintain genomic integrity during the entire cell cycle. The Ku70/80 complex recognizes DNA bre
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f40b14b079a79a20ee0bc05ad0c8ff7b
https://www.repository.cam.ac.uk/handle/1810/325720
https://www.repository.cam.ac.uk/handle/1810/325720
Autor:
Václav Matoušek, Zdeněk Kukačka, Daniel Pokorný, Iveta Klimánková, Kheironnesae Rahimidashaghoul, Michal Korecký, Matúš Chvojka, Martin Hubálek, Daniel Kavan, Petr Novák, Petr Beier, Lukáš Fojtík
Publikováno v:
Chemistry – A European Journal. 25:15779-15785
A series of fluoroalkylated cyclic λ3 -iodanes and their hydrochloride salts was prepared and used in a combination with sodium ascorbate in buffer or aqueous methanol mixtures for radical fluoroalkylation of a range of substituted indoles, pyrroles
Autor:
Tom L. Blundell, Petr Novák, Roland Beckmann, Zdeněk Kukačka, Thanh Binh Nguyen, Lukas Kater, David B. Ascher, Amanda K. Chaplin, Petr Tesina, Aleš Hnízda
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::972a0e553d956d8044e0e7d056621664
https://doi.org/10.1111/febs.15732/v2/response1
https://doi.org/10.1111/febs.15732/v2/response1
Publikováno v:
Journal of Proteome Research
Chemical cross-linking mass spectrometry has become a popular tool in structural biology. Although several algorithms exist that efficiently analyze data-dependent mass spectrometric data, the algorithm to identify and quantify intermolecular cross-l