Výsledky vyhledávání - "Zakia Morichaud"

Akademický článek

Structural basis of the mycobacterial stress-response RNA polymerase auto-inhibition via oligomerization

Autor: Zakia Morichaud, Stefano Trapani, Rishi K. Vishwakarma, Laurent Chaloin, Corinne Lionne, Joséphine Lai-Kee-Him, Patrick Bron, Konstantin Brodolin

Publikováno v: Nature Communications, Vol 14, Iss 1, Pp 1-13 (2023)

Biological processes can be regulated via oligomerization of macromolecules into high-order symmetric structures. Here, authors reported high-order structure of RNA polymerase and its role in regulation of gene expression in pathogenic bacterium.

Externí odkaz: https://doaj.org/article/f19847d7077247c59f06a06119b3f48c

Zobrazit plný text záznamu

Akademický článek

Pausing controls branching between productive and non-productive pathways during initial transcription in bacteria

Autor: David Dulin, David L. V. Bauer, Anssi M. Malinen, Jacob J. W. Bakermans, Martin Kaller, Zakia Morichaud, Ivan Petushkov, Martin Depken, Konstantin Brodolin, Andrey Kulbachinskiy, Achillefs N. Kapanidis

Publikováno v: Nature Communications, Vol 9, Iss 1, Pp 1-12 (2018)

RNA synthesis by bacterial RNA polymerase is interrupted by pauses but their role in RNA synthesis is poorly understood. Here the authors use single-molecule FRET and biochemical analysis to show that pausing regulates branching between the abortive

Externí odkaz: https://doaj.org/article/5a7bdeadb61d4f6d961109f5e5564849

Zobrazit plný text záznamu

Structural basis of the mycobacterial stress-response RNA polymerase auto-inhibition via oligomerization

Autor: Zakia Morichaud, Stefano Trapani, Rishi K. Vishwakarma, Laurent Chaloin, Corinne Lionne, Joséphine Lai-Kee-Him, Patrick Bron, Konstantin Brodolin

Self-assembly of macromolecules into higher-order symmetric structures is fundamental for the regulation of biological processes. Higher-order symmetric structure self-assembly by the gene expression machinery, such as bacterial DNA-dependent RNA pol

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0940f70c77f0481ac80a7d45a292c511
https://doi.org/10.1101/2022.07.04.498644

Zobrazit plný text záznamu

Modular Imaging Scaffold for Single-Particle Electron Microscopy

Autor: Joséphine Lai-Kee-Him, Nathalie Declerck, Gaëtan Bellot, Remy Sounier, Nesrine Aissaoui, Virginie Ropars, Jean-Baptiste Charbonnier, Zakia Morichaud, Patrick Bron, Sébastien Granier, Sonia Baconnais, Eric Le Cam, Konstantin Brodolin, Allan Mills

Publikováno v: ACS Nano. 15:4186-4196

Technological breakthroughs in electron microscopy (EM) have made it possible to solve structures of biological macromolecular complexes and to raise novel challenges, specifically related to sample preparation and heterogeneous macromolecular assemb

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::011b47e8411373cb6091772bbd68ce4f
https://doi.org/10.1021/acsnano.0c05113

Zobrazit plný text záznamu

Anti-pausing activity of region 4 of the RNA polymerase σ subunit and its regulation by σ-remodeling factors

Autor: Konstantin Brodolin, Zakia Morichaud

The basal transcription factors of cellular RNA polymerases (RNAPs) stimulate the initial RNA synthesis via poorly understood mechanisms. Here, we explored the mechanism employed by the bacterial factor σ in promoter-independent initial transcriptio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::11f6614a4ebd052f9f97b4258f452174
https://doi.org/10.1101/2020.08.11.244855

Zobrazit plný text záznamu

Region 4 of the RNA polymerase σ subunit counteracts pausing during initial transcription

Autor: Zakia Morichaud, Konstantin Brodolin

Publikováno v: The Journal of Biological Chemistry

All cellular genetic information is transcribed into RNA by multisubunit RNA polymerases (RNAPs). The basal transcription initiation factors of cellular RNAPs stimulate the initial RNA synthesis via poorly understood mechanisms. Here, we explored the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c41d827c88aca68e69d9fe02f0322487
https://doi.org/10.1074/jbc.ra120.016299

Zobrazit plný text záznamu

RbpA relaxes promoter selectivity of M. tuberculosis RNA polymerase

Autor: Ayyappasamy Sudalaiyadum Perumal, Yangbo Hu, Rishi K. Vishwakarma, Konstantin Brodolin, Zakia Morichaud

Publikováno v: Nucleic Acids Research
Nucleic Acids Research, 2018, 46 (19), pp.10106-10118. ⟨10.1093/nar/gky714⟩
Nucleic Acids Research, Oxford University Press, 2018, 46 (19), pp.10106-10118. ⟨10.1093/nar/gky714⟩

International audience; The transcriptional activator RbpA associates with Mycobacterium tuberculosis RNA polymerase (MtbRNAP) during transcription initiation, and stimulates formation of the MtbRNAP-promoter open complex (RPo). Here, we explored the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d6732924cc26cb32a373e615c3c89cb5
https://pubmed.ncbi.nlm.nih.gov/30102406

Zobrazit plný text záznamu

Pausing controls branching between productive and non-productive pathways during initial transcription

Autor: Andrey Kulbachinskiy, Martin Depken, Konstantin Brodolin, Martin Kaller, Achillefs N. Kapanidis, Bauer Dlv, Ivan Petushkov, Anssi M. Malinen, Jacob Bakermans, Zakia Morichaud, David Dulin

Transcription in bacteria is controlled by multiple molecular mechanisms that precisely regulate gene expression. Recently, initial RNA synthesis by the bacterial RNA polymerase (RNAP) has been shown to be interrupted by pauses; however, the pausing

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bdd03ce2d9726c6593765c0f7ff0b660

Zobrazit plný text záznamu

Single-molecule analysis reveals the mechanism of transcription activation in

Autor: Rishi Kishore, Vishwakarma, Anne-Marinette, Cao, Zakia, Morichaud, Ayyappasamy Sudalaiyadum, Perumal, Emmanuel, Margeat, Konstantin, Brodolin

Publikováno v: Science Advances

Mycobacterial activator RbpA stimulates transcription by stabilizing open conformation of the RNA polymerase σ subunit.
The σ subunit of bacterial RNA polymerase (RNAP) controls recognition of the −10 and −35 promoter elements during trans

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::c71d4188e175f2b23dd96401a141c515
https://pubmed.ncbi.nlm.nih.gov/29806016

Zobrazit plný text záznamu

Conformational heterogeneity and bubble dynamics in single bacterial transcription initiation complexes

Autor: Diego, Duchi, Kristofer, Gryte, Nicole C, Robb, Zakia, Morichaud, Carol, Sheppard, Konstantin, Brodolin, Sivaramesh, Wigneshweraraj, Achillefs N, Kapanidis

Publikováno v: Nucleic Acids Research

Transcription initiation is a major step in gene regulation for all organisms. In bacteria, the promoter DNA is first recognized by RNA polymerase (RNAP) to yield an initial closed complex. This complex subsequently undergoes conformational changes r

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::b7ff63dee0f9d4027a71ee78510418f3
https://pubmed.ncbi.nlm.nih.gov/29177430

Zobrazit plný text záznamu

Vyhledávací nástroje:

Upřesnit hledání