Protocol for engineering binding domains to recognize ligand-bound receptors by using yeast surface display.

Autor: Dobersberger M; Department of Chemistry, Institute of Biochemistry, BOKU University, Vienna 1190, Austria., Sumesgutner D; Department of Chemistry, Institute of Biochemistry, BOKU University, Vienna 1190, Austria; CD Laboratory for Next Generation CAR T Cells, Vienna 1090, Austria. Electronic address: delia.sumesgutner@boku.ac.at., Zajc CU; Department of Chemistry, Institute of Biochemistry, BOKU University, Vienna 1190, Austria; CD Laboratory for Next Generation CAR T Cells, Vienna 1090, Austria., Traxlmayr MW; Department of Chemistry, Institute of Biochemistry, BOKU University, Vienna 1190, Austria; CD Laboratory for Next Generation CAR T Cells, Vienna 1090, Austria. Electronic address: michael.traxlmayr@boku.ac.at.

Publikováno v: STAR protocols [STAR Protoc] 2024 Sep 24; Vol. 5 (4), pp. 103339. Date of Electronic Publication: 2024 Sep 24.

An engineering strategy to target activated EGFR with CAR T cells.

Autor: Dobersberger M; Department of Chemistry, Institute of Biochemistry, BOKU University, 1190 Vienna, Austria., Sumesgutner D; Department of Chemistry, Institute of Biochemistry, BOKU University, 1190 Vienna, Austria; CD Laboratory for Next Generation CAR T Cells, 1090 Vienna, Austria., Zajc CU; Department of Chemistry, Institute of Biochemistry, BOKU University, 1190 Vienna, Austria; CD Laboratory for Next Generation CAR T Cells, 1090 Vienna, Austria., Salzer B; CD Laboratory for Next Generation CAR T Cells, 1090 Vienna, Austria; St. Anna Children's Cancer Research Institute, CCRI, 1090 Vienna, Austria., Laurent E; BOKU Core Facility Biomolecular & Cellular Analysis, BOKU University, 1190 Vienna, Austria., Emminger D; CD Laboratory for Next Generation CAR T Cells, 1090 Vienna, Austria; St. Anna Children's Cancer Research Institute, CCRI, 1090 Vienna, Austria., Sylvander E; CD Laboratory for Next Generation CAR T Cells, 1090 Vienna, Austria; St. Anna Children's Cancer Research Institute, CCRI, 1090 Vienna, Austria., Lehner E; Department of Chemistry, Institute of Biochemistry, BOKU University, 1190 Vienna, Austria; CD Laboratory for Next Generation CAR T Cells, 1090 Vienna, Austria., Teufl M; Department of Chemistry, Institute of Biochemistry, BOKU University, 1190 Vienna, Austria; CD Laboratory for Next Generation CAR T Cells, 1090 Vienna, Austria., Seigner J; Department of Chemistry, Institute of Biochemistry, BOKU University, 1190 Vienna, Austria; Department of Biotechnology, Institute of Animal Cell Technology and Systems Biology, BOKU University, 1190 Vienna, Austria., Bobbili MR; Department of Biotechnology, Institute of Molecular Biotechnology, BOKU University, 1190 Vienna, Austria; Ludwig Boltzmann Institute for Traumatology, Research Center in Cooperation with AUVA, 1200 Vienna, Austria., Kunert R; Department of Biotechnology, Institute of Animal Cell Technology and Systems Biology, BOKU University, 1190 Vienna, Austria., Lehner M; CD Laboratory for Next Generation CAR T Cells, 1090 Vienna, Austria; St. Anna Children's Cancer Research Institute, CCRI, 1090 Vienna, Austria; St. Anna Children's Hospital, Department of Pediatrics, Medical University of Vienna, 1090 Vienna, Austria., Traxlmayr MW; Department of Chemistry, Institute of Biochemistry, BOKU University, 1190 Vienna, Austria; CD Laboratory for Next Generation CAR T Cells, 1090 Vienna, Austria. Electronic address: michael.traxlmayr@boku.ac.at.

Publikováno v: Cell reports methods [Cell Rep Methods] 2024 Apr 22; Vol. 4 (4), pp. 100728. Date of Electronic Publication: 2024 Mar 15.

Engineering Strategies to Overcome the Stability-Function Trade-Off in Proteins.

Autor: Teufl M; Department of Chemistry, Institute of Biochemistry, University of Natural Resources and Life Sciences, 1190 Vienna, Austria.; CD Laboratory for Next Generation CAR T Cells, 1190 Vienna, Austria., Zajc CU; Department of Chemistry, Institute of Biochemistry, University of Natural Resources and Life Sciences, 1190 Vienna, Austria.; CD Laboratory for Next Generation CAR T Cells, 1190 Vienna, Austria., Traxlmayr MW; Department of Chemistry, Institute of Biochemistry, University of Natural Resources and Life Sciences, 1190 Vienna, Austria.; CD Laboratory for Next Generation CAR T Cells, 1190 Vienna, Austria.

Publikováno v: ACS synthetic biology [ACS Synth Biol] 2022 Mar 18; Vol. 11 (3), pp. 1030-1039. Date of Electronic Publication: 2022 Mar 08.

Driving CARs with alternative navigation tools - the potential of engineered binding scaffolds.

Autor: Zajc CU; Christian Doppler Laboratory for Next Generation CAR T Cells, Vienna, Austria.; Department of Chemistry, Institute of Biochemistry, BOKU-University of Natural Resources and Life Sciences, Vienna, Austria., Salzer B; Christian Doppler Laboratory for Next Generation CAR T Cells, Vienna, Austria.; St. Anna Children's Cancer Research Institute, Vienna, Austria., Taft JM; Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland., Reddy ST; Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland., Lehner M; Christian Doppler Laboratory for Next Generation CAR T Cells, Vienna, Austria.; St. Anna Children's Cancer Research Institute, Vienna, Austria.; Department of Pediatrics, St. Anna Kinderspital, Medical University of Vienna, Austria., Traxlmayr MW; Christian Doppler Laboratory for Next Generation CAR T Cells, Vienna, Austria.; Department of Chemistry, Institute of Biochemistry, BOKU-University of Natural Resources and Life Sciences, Vienna, Austria.

Publikováno v: The FEBS journal [FEBS J] 2021 Apr; Vol. 288 (7), pp. 2103-2118. Date of Electronic Publication: 2020 Aug 31.

A conformation-specific ON-switch for controlling CAR T cells with an orally available drug.

Autor: Zajc CU; St. Anna Children's Cancer Research Institute (CCRI), 1090 Vienna, Austria.; Christian Doppler Laboratory for Next Generation CAR T Cells, 1090 Vienna, Austria., Dobersberger M; St. Anna Children's Cancer Research Institute (CCRI), 1090 Vienna, Austria., Schaffner I; BOKU Core Facility Biomolecular & Cellular Analysis, BOKU-University of Natural Resources and Life Sciences, 1190 Vienna, Austria., Mlynek G; Department of Structural and Computational Biology, Max Perutz Labs, University of Vienna, 1030 Vienna, Austria., Pühringer D; Department of Structural and Computational Biology, Max Perutz Labs, University of Vienna, 1030 Vienna, Austria., Salzer B; St. Anna Children's Cancer Research Institute (CCRI), 1090 Vienna, Austria.; Christian Doppler Laboratory for Next Generation CAR T Cells, 1090 Vienna, Austria., Djinović-Carugo K; Department of Structural and Computational Biology, Max Perutz Labs, University of Vienna, 1030 Vienna, Austria.; Department of Biochemistry, Faculty of Chemistry and Chemical Technology, University of Ljubljana, 1000 Ljubljana, Slovenia., Steinberger P; Institute of Immunology, Medical University of Vienna, 1090 Vienna, Austria., De Sousa Linhares A; Institute of Immunology, Medical University of Vienna, 1090 Vienna, Austria., Yang NJ; Department of Immunology, Harvard Medical School, Boston, MA 02115., Obinger C; Department of Chemistry, Institute of Biochemistry, BOKU-University of Natural Resources and Life Sciences, 1190 Vienna, Austria., Holter W; St. Anna Children's Cancer Research Institute (CCRI), 1090 Vienna, Austria.; St. Anna Kinderspital, Department of Pediatrics, Medical University of Vienna, 1090 Vienna, Austria., Traxlmayr MW; Christian Doppler Laboratory for Next Generation CAR T Cells, 1090 Vienna, Austria; michael.traxlmayr@boku.ac.at manfred.lehner@ccri.at.; Department of Chemistry, Institute of Biochemistry, BOKU-University of Natural Resources and Life Sciences, 1190 Vienna, Austria., Lehner M; St. Anna Children's Cancer Research Institute (CCRI), 1090 Vienna, Austria; michael.traxlmayr@boku.ac.at manfred.lehner@ccri.at.; Christian Doppler Laboratory for Next Generation CAR T Cells, 1090 Vienna, Austria.; St. Anna Kinderspital, Department of Pediatrics, Medical University of Vienna, 1090 Vienna, Austria.

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2020 Jun 30; Vol. 117 (26), pp. 14926-14935. Date of Electronic Publication: 2020 Jun 17.