A simple, versatile and sensitive cell-based assay for prions from various species.

Autor: Zaira E Arellano-Anaya, Jimmy Savistchenko, Jacinthe Mathey, Alvina Huor, Caroline Lacroux, Olivier Andréoletti, Didier Vilette

Publikováno v: PLoS ONE, Vol 6, Iss 5, p e20563 (2011)

Detection and quantification of prion infectivity is a crucial step for various fundamental and applied aspects of prion research. Identification of cell lines highly sensitive to prion infection led to the development of cell-based titration procedu

Externí odkaz: https://doaj.org/article/36c76f47a2734910aeeb030828dbb6ab

Production of seedable Amyloid-β peptides in model of prion diseases upon PrPSc-induced PDK1 overactivation

Autor: Juliette Ezpeleta, Vincent Baudouin, Zaira E. Arellano-Anaya, François Boudet-Devaud, Mathéa Pietri, Anne Baudry, Anne-Marie Haeberlé, Yannick Bailly, Odile Kellermann, Jean-Marie Launay, Benoit Schneider

Publikováno v: Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019)

Aβ plaques have been detected in brains of patients with prion diseases. Here, using mice, the authors show that prion infection enhances Aβ production via a PDK1-TACE mechanism and that brain deposition of Aβ induced by Aβ seeds co-transmitted w

Externí odkaz: https://doaj.org/article/1e4e707388e247a5aac2217f5c04838e

Production of seedable Amyloid-β peptides in model of prion diseases upon PrPSc-induced PDK1 overactivation

Autor: Zaira E. Arellano-Anaya, Yannick Bailly, Anne Baudry, Mathéa Pietri, François Boudet-Devaud, Jean-Marie Launay, Vincent Baudouin, Juliette Ezpeleta, Odile Kellermann, Benoit Schneider, Anne-Marie Haeberlé

Publikováno v: Nature Communications
Nature Communications, Nature Publishing Group, 2019, 10 (1), ⟨10.1038/s41467-019-11333-3⟩
Nature Communications, 2019, 10 (1), ⟨10.1038/s41467-019-11333-3⟩
Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019)

The presence of amyloid beta (Aβ) plaques in the brain of some individuals with Creutzfeldt-Jakob or Gertsmann-Straussler-Scheinker diseases suggests that pathogenic prions (PrPSc) would have stimulated the production and deposition of Aβ peptides.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dfd47427da6f761514a5ecd1acf9fb32
https://hal.archives-ouvertes.fr/hal-02363104/document

Isolation of Exosomes and Microvesicles from Cell Culture Systems to Study Prion Transmission

Autor: Didier Vilette, Graça Raposo, Laurent Schaeffer, Monique Provansal, Emilien Bernard, Sylvain Lehmann, Zaira E. Arellano-Anaya, Laure Gallay, Pascal Leblanc

Publikováno v: Methods Mol Biol
Methods Mol Biol, pp.153-176, 2017, ⟨10.1007/978-1-4939-6728-5_11⟩
Methods in Molecular Biology ISBN: 9781493967261

Extracellular vesicles (EVs) are composed of microvesicles and exosomes. Exosomes are small membrane vesicles (40-120 nm sized) of endosomal origin released in the extracellular medium from cells when multivesicular bodies fuse with the plasma membra

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ebe44d0049ec1139b92beac77823f8a2
https://hal.science/hal-02328354

Expression of Heterologous PrP and Prion Propagation in RK13 Cells

Autor: Zaira E. Arellano-Anaya, Olivier Andréoletti, Didier Vilette, Pascal Leblanc, Alvina Huor

Publikováno v: Methods Mol Biol
Methods Mol Biol, pp.95-104, 2017, ⟨10.1007/978-1-4939-7244-9_8⟩
Prions ISBN: 9781493972425

Cultured cells are valuable models to study prion infections at the cellular level. Unfortunately, the vast majority of cell lines are resistant to the propagation of prion agents. The rabbit epithelial RK13 cell line is among the few cell lines perm

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a35d8bca7b8f951ff4be10af3a64dce9
https://hal.archives-ouvertes.fr/hal-02328311