Low-cost anti-mycobacterial drug discovery using engineered E. coli

Autor: Nadine Bongaerts, Zainab Edoo, Ayan A. Abukar, Xiaohu Song, Sebastián Sosa-Carrillo, Sarah Haggenmueller, Juline Savigny, Sophie Gontier, Ariel B. Lindner, Edwin H. Wintermute

Publikováno v: Nature Communications, Vol 13, Iss 1, Pp 1-11 (2022)

Whole-cell screening for Mycobacterium tuberculosis inhibitors is complicated by the pathogen’s slow growth and biocontainment requirements. Here the authors develop engineered E. coli as a synthetic biology tool to express and screen metabolic tar

Externí odkaz: https://doaj.org/article/954339c29a5245b5a82434151be0eb50

Activity-Based Protein Profiling Reveals That Cephalosporins Selectively Active on Non-replicating Mycobacterium tuberculosis Bind Multiple Protein Families and Spare Peptidoglycan Transpeptidases

Autor: Landys Lopez Quezada, Robert Smith, Tania J. Lupoli, Zainab Edoo, Xiaojun Li, Ben Gold, Julia Roberts, Yan Ling, Sae Woong Park, Quyen Nguyen, Frank J. Schoenen, Kelin Li, Jean-Emmanuel Hugonnet, Michel Arthur, James C. Sacchettini, Carl Nathan, Jeffrey Aubé

Publikováno v: Frontiers in Microbiology, Vol 11 (2020)

As β-lactams are reconsidered for the treatment of tuberculosis (TB), their targets are assumed to be peptidoglycan transpeptidases, as verified by adduct formation and kinetic inhibition of Mycobacterium tuberculosis (Mtb) transpeptidases by carbap

Externí odkaz: https://doaj.org/article/4d560f0f64cb40cc8c57181a0b7ea41e

Click and Release Chemistry for Activity-Based Purification of β-Lactam Targets

Autor: Saidbakhrom Saidjalolov, Filippo Rusconi, Zainab Edoo, Emmanuelle Braud, Margaux Riomet, Frédéric Taran, Antoine Sallustrau, Mélanie Etheve-Quelquejeu, Michel Arthur, Matthieu Fonvielle, Laura Iannazzo

Publikováno v: Chemistry-A European Journal
Chemistry-A European Journal, Wiley-VCH Verlag, 2021, 27 (28), pp.7687-7695. ⟨10.1002/chem.202100653⟩
Chemistry-A European Journal, 2021, 27 (28), pp.7687-7695. ⟨10.1002/chem.202100653⟩

International audience; β-Lactams, the cornerstone of antibiotherapy, inhibit multiple and partially redundant targets referred to as transpeptidases or penicillin-binding proteins. These enzymes catalyze the essential cross-linking step of the poly

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1e1b9d2420e7d5831966798eaab635b9
https://hal.sorbonne-universite.fr/hal-03281379/document

Synthesis of carbapenems containing peptidoglycanmimetics and inhibition of the cross-linking activity of a transpeptidase of the L,D specificity

Autor: Saidbakhrom Saidjalolov, Matthieu Fonvielle, Mélanie Etheve-Quelquejeu, Laura Iannazzo, Michel Arthur, Emmanuelle Braud, Louis Mayer, Zainab Edoo

Publikováno v: Chemistry-A European Journal
Chemistry-A European Journal, Wiley-VCH Verlag, 2021, 27 (10), pp.3542-3551. ⟨10.1002/chem.202004831⟩
Chemistry-A European Journal, 2021, 27 (10), pp.3542-3551. ⟨10.1002/chem.202004831⟩

International audience; The carbapenem class of β-lactams has been optimized against Gram-negative bacteria producing extended-spectrum β-lactamases by introducing substituents at position C2. Carbapenems are currently investigated for the treatmen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::49eee50895238295f1342b558ff32db8
https://hal.sorbonne-universite.fr/hal-03184204

Inhibition Activity of Avibactam against Nocardia farcinica β-Lactamase FAR IFM10152

Autor: Fabrice Compain, Clément Ourghanlian, Delphine Dorchène, Michel Arthur, Daria Soroka, David Lebeaux, Zainab Edoo

Publikováno v: Antimicrobial Agents and Chemotherapy. 64

Nocardia farcinica, one of the most frequent pathogenic species responsible for nocardiosis, is characterized by frequent brain involvement and resistance to β-lactams mediated by a class A β-lactamase. Kinetic parameters for hydrolysis of various

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::4802ba3e141b981c0726041795589b2f
https://doi.org/10.1128/aac.01551-19