A spiroligomer α-helix mimic that binds HDM2, penetrates human cells and stabilizes HDM2 in cell culture.

Autor: Zachary Z Brown, Kavitha Akula, Alla Arzumanyan, Jennifer Alleva, Marcus Jackson, Eugeney Bichenkov, Joel B Sheffield, Mark A Feitelson, Christian E Schafmeister

Publikováno v: PLoS ONE, Vol 7, Iss 10, p e45948 (2012)

We demonstrate functionalized spiroligomers that mimic the HDM2-bound conformation of the p53 activation domain. Spiroligomers are stereochemically defined, functionalized, spirocyclic monomers coupled through pairs of amide bonds to create spiro-lad

Externí odkaz: https://doaj.org/article/bf935dddc2744099b5b565667ce91045

Genomic targeting of epigenetic probes using a chemically tailored Cas9 system

Autor: Zachary Z. Brown, Rob C. Oslund, Yael David, Samuel H. Kim, Tom W. Muir, Glen Liszczak

Publikováno v: Proceedings of the National Academy of Sciences. 114:681-686

Recent advances in the field of programmable DNA-binding proteins have led to the development of facile methods for genomic localization of genetically encodable entities. Despite the extensive utility of these tools, locus-specific delivery of synth

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2f8585caf2f173db0c56ca78b918169a
https://doi.org/10.1073/pnas.1615723114

Design of a Split Intein with Exceptional Protein Splicing Activity

Autor: Zachary Z. Brown, Tom W. Muir, David Cowburn, Adam J. Stevens, Giridhar Sekar, Neel H. Shah

Publikováno v: Journal of the American Chemical Society

Protein trans-splicing (PTS) by split inteins has found widespread use in chemical biology and biotechnology. Herein, we describe the use of a consensus design approach to engineer a split intein with enhanced stability and activity that make it more

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba423cd3c1d0ad0b7066fb1fe857920f
https://doi.org/10.1021/jacs.5b13528

ISWI chromatin remodellers sense nucleosome modifications to determine substrate preference

Autor: Zachary Z. Brown, Samuel B. Pollock, Katharine L. Diehl, C. David Allis, Rasmus Pihl, Geoffrey P. Dann, Felix Wojcik, Uyen T. T. Nguyen, Glen Liszczak, Manuel M. Müller, Jeffrey Bos, Tatyana Panchenko, Tom W. Muir, John D. Bagert

Publikováno v: Dann, G P, Liszczak, G, Bagert, J D, Mueller, M M, Nguyen, U TT, Wojcik, F, Brown, Z Z, Bos, J, Pachenko, T, Pihl, R, Pollock, S B, Diehl, K L, Allis, C D & Muir, T W 2017, ' ISWI chromatin remodellers sense nucleosome modifications to determine substrate preference ', NATURE, vol. 548, no. 7669, pp. 607-611 . https://doi.org/10.1038/nature23671
Dann, G P, Liszczak, G P, Bagert, J D, Müller, M M, Nguyen, U T T, Wojcik, F, Brown, Z Z, Bos, J, Panchenko, T, Pihl, R, Pollock, S B, Diehl, K L, Allis, C D & Muir, T W 2017, ' ISWI chromatin remodellers sense nucleosome modifications to determine substrate preference ', Nature, vol. 548, no. 7669, pp. 607-611 . https://doi.org/10.1038/nature23671

ATP-dependent chromatin remodellers regulate access to genetic information by controlling nucleosome positions in vivo1. However, the mechanism by which remodellers discriminate between different nucleosome substrates is poorly understood. Many chrom

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::26bb907bed5f1dd15166570c8636256c
https://europepmc.org/articles/PMC5777669/

Surface-attached molecules control Staphylococcus aureus quorum sensing and biofilm development

Autor: Aishan Zhao, Zachary Z. Brown, Tom W. Muir, Bonnie L. Bassler, Min-Young Kim, Ashley Wang, Howard A. Stone

Publikováno v: Nature microbiology

Bacteria use a process called quorum sensing to communicate and orchestrate collective behaviours, including virulence factor secretion and biofilm formation. Quorum sensing relies on the production, release, accumulation and population-wide detectio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3e93e9142910738f25e46d8b87adf57e
https://pubmed.ncbi.nlm.nih.gov/28530651